Agrobacterium tumefaciens-mediated transformation of Allium cepa L.: The production of transgenic onions and shallots

Molecular Breeding

Volumn 7, Issue 2, 2001, Pages 101-115

  Zheng, Si Jun ^a   Khrustaleva, Ludmila ^a   Henken, Betty ^a   Sofiari, Eri ^b   Jacobsen, Evert ^a   Kik, Chris ^b   Krens, Frans A ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b Research Institute for Vegetables (RIV AARD)   (Indonesia)

Author keywords

Agrobacterium tumefaciens; Allium cepa; FISH (fluorescence in situ hybridization); Transformation; Transgenic plant characterization

Indexed keywords

BETA GLUCURONIDASE; CALLUS CULTURE; CHROMOSOMAL LOCALIZATION; COCULTURE; CULTIVAR; DIPLOIDY; DNA TRANSFER; FLUORESCENCE IN SITU HYBRIDIZATION; GENE INSERTION; GENETIC LINE; GENETIC TRANSFORMATION; GREENHOUSE; HYGROMYCIN; SOUTHERN BLOTTING; TRANSGENIC PLANT; ZYGOTE;

BACTERIA; EFFICIENCY; FISH; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; INTEGRATION; PLANTS (BOTANY); POLYMERASE CHAIN REACTION;

AGROBACTERIUM TUMEFACIENS; ALLIUM CEPA; CO-CULTIVATION; FLUORESCENCE IN SITU HYBRIDIZATION; PLANTLETS; TRANSFORMATION; TRANSGENEIC PLANTS; TRANSGENIC PLANT CHARACTERIZATION; ZYGOTIC EMBRYOS;

DNA;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; ALLIUM ASCALONICUM; ALLIUM CEPA; EUKARYOTA; RADIOBACTER; RHIZOBIUM;

EID: 0034914368     PISSN: 13803743     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1011348229189     Document Type: Article

References (49)

1. Agrobacterium tumefaciens-mediated transformation of japonica and indica rice varieties
2. An efficient protocol for sugarcane (Saccharum spp. L.) transformation mediated by Agrobacterium tumefaciens
3. Biolistic transfer and expression of a uidA reporter gene in different tissues of Allium sativum L.
4. Ectopic pairing of homologous DNA and post-transcriptional gene silencing in transgenic plants
5. Agrobacterium-mediated production of transgenic rice plants expressing a chimeric alpha-amylase promoter/βglucuronidase gene
6. Genetic transformation of wheat mediated by Agrobacterium tumefaciens
7. Agrobacterium-transformed rice plants expressing synthetic cryIA(b) and cryIA(c) genes are highly toxic to striped stem borer and yellow stem borer
8. Onion is a monocotyledonous host for Agrobacterium
9. Agrobacterium-mediated transformation of javanica rice
10. Towards the transformation of onions (Allium cepa)
11. A comparison of four selective agents for use with Allium cepa L. immature embryos and immature embryo derived cultures
12. Transient expression of uidA constructs in in vitro onion (Allium cepa L.) cultures following panicle bombardment and Agrobacterium-mediated DNA delivery
13. Agrobacterium tumefaciens-mediated transformation and transgenic-plant regeneration of onion (Allium cepa L.)
14. Herbicide-resistant sugarcane (Saccharum officinarum L.) plants by Agrobacterium-mediated transformation
15. The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species
16. Regeneration of transgenic cassava plants (Manihot esculenta Crantz) through Agrobacterium-mediated transformation of embryogenic suspension cultures
17. Factors influencing T-DNA transfer into wheat and barley cells by Agrobacterium tumefaciens
18. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA
19. Transformation of rice mediated by Agrobacterium tumefaciens
20. A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid
21. The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA
22. New Agrobacterium helper plasmids for gene transfer to plants
23. Transformation of plant cells via Agrobacterium
24. High efficiency transformation of maize (Zea may L.) mediated by Agrobacterium tumefaciens
25. Assaying chimeric genes in plants: The GUS gene fusion system
26. Influence of phenolic compounds on Agrobacterium vir gene induction and onion gene transfer
27. Agrobacterium-mediated transformation of indica rice cultivars using binary and superbinary vectors
28. Introgression of Allium fistulosum into A. cepa mediated by A. roylei
29. High-velocity microprojectiles for delivering nucleic acids into living cells
30. Transformation of cultured cells of Chenopodium quinoa by binary vectors that carry a fragment of DNA from the virulence region of pTiBo542
31. Genetic transformation of cassava (Manihot esculerta Crantz)
32. Factors influencing Agrobacterium-mediated transient expression of gusA in rice
33. A highly efficient transformation system mediated by Agrobacterium tumefaciens in rice (Oryza sativa L.)
34. Homology-dependent gene silencing in plants
35. Construction and expression in tobacco of a β-glucuronidase (GUS) reporter gene containing an intron within the coding sequence
36. T-DNA integration into genomic DNA of rice following Agrobacterium inoculation of isolated shoot apices
37. Agrobacterium-mediated transformation of rice (Oryza sativa L.)
38. Transgenic plant production mediated by Agrobacterium in indica rice
39. Model system for plant cell biology: GFP imaging in living onion epidermal cells
40. Advances in alternative DNA delivery techniques
41. Agrobacterium tumefaciens-mediated barley transformation
42. Rapid and efficient Agrobacterium-mediated transformation in rice
43. Transgenic rice: A valuable monocot system for crop improvement and gene research
44. Plasmolysis of precultured immature embryos improves Agrobacterium mediated gene transfer to rice (Oryza sativa L.)
45. A genetic map of an interspecific cross in Allium based on amplified fragment length polymorphism (AFLP™) markers
46. A model for RNA mediated gene silencing in higher plants
47. Factors influencing induction, propagation and regeneration of mature zygotic embryo-derived callus from Allium cepa
48. Effect of cytokinins and lines on plant regeneration from long-term callus and suspension cultures of Allium cepa L.