Evaluation of cryoinjury of spermatozoa after slow (programmed biological freezer) or rapid (liquid nitrogen vapour) freeze-thawing techniques

Journal of Assisted Reproduction and Genetics

Volumn 18, Issue 7, 2001, Pages 364-370

  Hammadeh, M E ^a   Szarvasy, D ^a   Zeginiadou, T ^a   Rosenbaum, P ^a   Georg, T ^b   Schmidt, W ^b  

^a Department of Obstetrics and Gynaecology ^*  (Germany)

^b SAARLAND UNIVERSITY   (Germany)

Author keywords

Computerized biological freezer; Freeze thawing procedure; Spermatozoa chromatin and morphology; Static liquid nitrogen

Indexed keywords

LIQUID NITROGEN;

ARTICLE; CELL TYPE; CHROMATIN; CRYOPRESERVATION; FREEZING; HUMAN; HUMAN CELL; INTRACYTOPLASMIC SPERM INJECTION; MALE; PRIORITY JOURNAL; SEMEN ANALYSIS; SPERMATOZOON; THAWING;

CELL SURVIVAL; CRYOPRESERVATION; FREEZING; HUMANS; MALE; SEMEN PRESERVATION; SPERMATOZOA;

EID: 0034910666     PISSN: 10580468     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1016666221500     Document Type: Article

References (53)

1. Survival of spermatozoa after vitrification and dehydration at low temperatures
2. General principles of cell preservation
3. Basic aspect of frozen storage of seme
4. Semen quality from the bull to the freezer: An assessment
5. Fundamentals of the preservation of spermatozoa
6. The preservation of semen in mammals
7. Equilibrium quasi-equilibrium and non equilibrium freezing of mammalian embryos
8. Kinetics of water loss from cells at subzero temperatures and the likelihood of intracellular freezing
9. Mechanism of injury and protection in living cells and tissues at low temperaturees
10. The osmotic rupture hypothesis of intracellular freezing injury
11. Effect of salts concentration and unfrozen water fraction on the viability of slowly frozen ram spermatozoa
12. The effect of cooling rate and repeated freezing on human sperm cryosurvival
13. Cryopreservation of human spermatozoa. IV. The effect of cooling rate and warming rate on the maintenance of motility, plasma membrane integrity, and mitochondrial function
14. Cryopreservation, actin localization and thermotropic phase transmsition in ram spermatozoa
15. Cryopreservation of mammalian sperm: What we ask them to survive
16. Evidence of increased lipid peroxidarive damage and loss of superoxide dismutase activity as a mode of sublethal cryodamage to human sperm during cryopreservation
17. Cryopreservation of human spermatozoa. II. Postthaw chronology of motility and of zona-free hamster ova penetration
18. Poor hypo-osmotic swelling test result from cryopreserved sperm despite preservation of sperm motility
19. Effect of cryopreservation on human sperm acrosomes
20. Association between freezing agent and acrosome damage of human spermatozoa from subnormal and normal semen
21. Cryopreservation of spermatozoa: A review
22. The evaluation of morphologic characteristics of human spermatozoa according to strict criteria
23. A test for the practical evaluation of male fertility by acridine orange (AO) fluorescence
24. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics
25. Supravital staining of human spermatozoa
26. A technique for studying the morphology of mammalian spermatozoa which are eosinophilic in a differential life dead stain
27. Cryopreservation of human semen: Comparison of cryopreservation, sources of variability, and prediction of post-thaw survival
28. Current status of clinical cryobanking of human sperm
29. A comparison of the freezing semen in the uncerculated vapour above liquid nitrogen and in a commercial semi-programmable freezer
30. Cryopreservation of human spermatozoa: Correlation of ultrastructural sperm head configuration with sperm motility and ability to penetrate zona free hamster ova
31. Computerized slow-staged freezing of semen from men with testicular tumours or Hodgkin's disease preserves sperm better than standard vapour freezing
32. Effect of freezing method, thawing temperature and post-thaw dilution/washing on motility (CASA) and morphology characteristics of high-quality human sperm
33. Preservation techniques for human semen
34. Sperm cryopreservation: State of the art
35. Detrimental effects of cryopreservation on structural and functional integrity of the sperm membrane
36. Further evaluation of a simple test for determining the integrity of spermatozoa membranes
37. The hypoosmotic swelling test and cryosurvival of human spermatozoa
38. Suitability of the hypo-osmotic swelling test for assessing the viability of cryopreserved sperm
39. Improved results of thawed sperm cryopreserved with slow stage cooling with a cellevator
40. Freezing and thawing alter chromatin stability of ejaculated human spermatozoa: Fluorescence acridine orange staining and Feulgen - DNA cytophotometrics studies
41. Effect of freezing on the spermatozoa nucleus: A comparative chromatin cytophotometric study in the porcine and human species
42. Elektrophoretic patterns of spermatozoal nucleoproteins (NP) in fertile men and infertile patients and comparison with NP of somatic cells
43. Sperm nuclear instability and staining with aniline blue: Abnormal persistence of histones in spermatozoa in infertile men
44. Effect of freeze-thawing procedure on chromatin stability, morphological alteration and membrane integrity of human spermatozoa in fertile and subfertile men
45. Light and electron microscopic analysis of human testicular spermatozoa and spermatids from frozen and thawed testicular biopsies
46. Ultrastructure changes in membrane and acrosomes of human sperm during cryopreservation
47. Chromatin alterations induced by freeze-thawing influence the fertilizing ability of human sperm
48. Comparison of the nuclear DNA stability against freezing-thawing and high temperature treatments between spermatozoa and somatic cells
49. Ultrastructure changes in the human spermatozoon after freezing for artificial insemination
50. Effect of cryopreservation on the motility characteristics of human spermatozoa
51. Effect of cooling, freezing and thawing rates and storage conditions on preservation of human spermatozoa
52. The contribution of the toxicity of a glycerol-egg yolk-citrate cryopreservative to the decline in human sperm motility during cryopreservation