Methylation of the Opaque2 box in zein genes is parent-dependent and affects O2 DNA binding activity in vitro

Plant Molecular Biology

Volumn 46, Issue 5, 2001, Pages 549-560

  Sturaro, Monica ^a   Viotti, Angelo ^a  

^a CNR   (Italy)

Author keywords

Endosperm; Maize; O2 box methylation; Opaque 2 bZIP; Zein

Indexed keywords

ELECTROPHORETIC MOBILITY; ENDOSPERM; GENE SEQUENCE; HOMEOBOX GENE; LEUCINE ZIPPER PROTEIN; METHYLATION; PROTEIN BINDING; PROTEIN EXPRESSION; TRANSACTIVATION; TRANSCRIPTION REGULATION; ZEIN;

ELECTROPHORESIS; GENES; PLANT CELL CULTURE; TISSUE;

ENDOSPERM;

ALKYLATION;

BASE SEQUENCE; BINDING SITES; DNA METHYLATION; DNA, PLANT; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, PLANT; MOLECULAR WEIGHT; OLIGONUCLEOTIDES; PLANT PROTEINS; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; TRANSCRIPTION FACTORS; ZEA MAYS; ZEIN;

EMBRYOPHYTA; ZEA MAYS;

EID: 0034903238     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1010686721797     Document Type: Article

References (44)

1. A maize ribosome-inactivating protein is controlled by the transcriptional activator Opaque-2
2. Molecular analysis of wild-type and mutant alleles at the Opaque-2 regulatory locus of maize reveals different mutations and types of O2 products
3. DNA methylation and tissue-specific transcription of storage protein genes of maize
4. The methyl-CpG binding transcriptional repressor MeCP2 stably associates with nucleosomal DNA
5. Phosphorylation of Opaque2 changes diurnally and impacts its DNA binding activity
6. Specific combinations of zein genes and genetic backgrounds influence the transcription of the heavy-chain zein genes in maize opaque-2 endosperms
7. Eukaryotic DNA methylation as an evolutionary device
8. The involvement of Opaque 2 on β-prolamin gene regulation in maize and Coix suggests a more general role for this transcriptional activator
9. An epigenetic mutation responsible for natural variation in floral symmetry
10. Characterization of novel proteins affected by the o2 mutation and expressed during maize endosperm development
11. Identification of a DNA-binding factor that recognizes an α-coixin promoter and interacts with a Coix Opaque-2 like protein
12. DNA methylation, a key regulator of plant development and other processes
13. A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands
14. Differences in cell type-specific expression of the gene Opaque 2 in maize and transgenic tobacco
15. The O2 gene which regulates zein deposition in maize endosperm encodes a protein with structural homologies to transcriptional activators
16. CpG methylation inhibits binding of several sequence-specific DNA-binding proteins from pea, wheat, soybean and cauliflower
17. Plant bZIP protein DNA binding specificity
18. How does DNA methylation repress transcription?
19. The role of opaque2 in the control of lysine-degrading activities in developing maize endosperm
20. The opaque-2 mutation of maize differentially reduces zein gene transcription
21. The maize regulatory locus Opaque-2 encodes a DNA-binding protein which activates the transcription of the b-32 gene
22. Translation of the mRNA of the maize transcriptional activator Opaque-2 is inhibited by upstream open reading frames present in the leader sequence
23. Maternal-specific demethylation and expression of specific alleles of zein genes in the endosperm of Zea mays L.
24. Endosperm-specific demethylation and activation of specific alleles of α-tubulin genes of Zea mays L.
25. The sequence of the zein regulatory gene opaque-2 (O2) of Zea mays
26. The transcriptional activator Opaque-2 controls the expression of a cytosolic form of pyruvate orthophosphate dikinase-1 in maize endosperms
27. Regulation of storage protein synthesis in cereal seeds: Developmental and nutritional aspects
28. The role of multiple binding sites in the activation of zein gene expression by Opaque-2
29. OHP1: A maize basic domain/leucine zipper protein that interacts with opaque2
30. Analysis of the methylation pattern of the maize opaque-2 (O2) promoter and in vitro binding studies indicate that the O2 B-Zip protein and other endosperm factors can bind to methylated target sequences
31. Genetic organization of zeins
32. Transposon tagging and molecular analysis of the maize regulatory locus opaque-2
33. Maize regulatory gene opaque-2 encodes a protein with a 'leucinezipper' motif that binds to zein DNA
34. Opaque-2 is a transcriptional activator that recognizes a specific target site in 22-kD zein genes
35. Zein level in maize endosperm depends on a protein under control of the opaque-2 and opaque-6 loci
36. Methylation of B-hordein genes in barley endosperm is inversely correlated with gene activity and affected by the regulatory gene lys3
37. Effects of DNA methylation on DNA-binding proteins and gene expression
38. Mutations of the 22- and 27-kD zein promoters affect transactivation by the Opaque-2 protein
39. A maize zinc-finger protein binds the prolamin box in zein gene promoters and interacts with the basic leucine zipper transcriptional activator Opaque2
40. Multiplicity and diversity of cloned zein cDNA sequences and their chromosomal location
41. Modulation of gene expression by DNA-protein and protein-protein interactions in the promoter region of the zein multigene family
42. Characterization of the maize prolamin box-binding factor-1 (PBF-1) and its role in the developmental regulation of the zein multigene family
43. The transcriptional activator Opaque2 recognizes two different target sequences in the 22-kD-like α-prolamin genes
44. Cooperative DNA binding and sequence discrimination by the Opaque2 bZIP factor