Polymerase chain reaction and denaturing gradient gel electrophoresis monitoring of fecal Bifidobacterium populations in a prebiotic and probiotic feeding trial

Systematic and Applied Microbiology

Volumn 24, Issue 2, 2001, Pages 227-231

  Satokari, R M ^a   Vaughan, E E ^a   Akkermans, A D L ^a   Saarela, M ^a   De Vos, W M ^a  

^a VTT TECHNICAL RESEARCH CENTRE OF FINLAND   (Finland)

Author keywords

Bifidobacterium spp; DGGE; Feeding trial; PCR; Prebiotic; Probiotic

Indexed keywords

ADULT; ARTICLE; BACTERIAL COLONIZATION; BACTERIUM IDENTIFICATION; BIFIDOBACTERIUM; BIFIDOBACTERIUM ANIMALIS; COLONY FORMING UNIT; CONTROLLED STUDY; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA SEQUENCE; FECES ANALYSIS; FECES CULTURE; FEMALE; HUMAN; HUMAN EXPERIMENT; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); BACTERIUM LACTIS; BIFIDOBACTERIUM; BIFIDOBACTERIUM ANIMALIS; BIFIDOBACTERIUM LACTIS; UNCULTURED ACTINOMYCETE;

EID: 0034894451     PISSN: 07232020     EISSN: None     Source Type: Journal    
DOI: 10.1078/0723-2020-00035     Document Type: Article

References (25)

1. Effect of galacto-oligosaccharide supplementation on human faecal microflora and on survival and persistence of Bifidobacterium lactis Bb-12 in the gastrointestinal tract
2. Persistence of colonization of human colonic mucosa by a probiotic strain, Lactobacillus rhamnosus GG, after oral consumption
3. Effect of transgalactooligosaccharides on the composition of the human intestinal microflora and on putative risk markers for colon cancer
4. Bifidobacteria and probiotic action
5. Effects of Bifidobacterium sp fermented milk ingested with or without inulin on colonic bifidobacteria and enzymatic activities in healthy humans
6. Specific detection of Bifidobacterium strains in a pharmaceutical probiotic product and in human feces by polymerase chain reaction
7. Monitoring and survival of Lactobacillus paracasei LTH 2579 in food and the human intestinal tract
8. Probiotics, prebiotics, and synbiotics: Approaches for modulating the microbial ecology of the gut
9. Prebiotics, probiotics and human gut microbiology
10. Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics
11. Comparison of viable cell counts and fluorescence in situ hybridization using specific rRNA-based probes for the quantification of human fecal bacteria
12. Interspecies differences in maximum specific growth rates and cell yields of bifidobacteria cultured on oligosaccharides and other simple carbohydrate sources
13. Differentiation of ingested and endogenous bifidobacteria by DNA fingerprinting demonstrates the survival of an unmodified strain in the gastrointestinal tract of humans
14. Bifidobacterium gallicum sp. nov. isolated from human feces
15. Molecular monitoring of human intestinal Bifidobacterium strain diversity
16. Molecular analysis of the composition of the bifidobacterial and lactobacillus microflora of humans
17. Tracking of probiotic bifidobacteria in the intestine
18. Probiotics: Mechanisms and established effects
19. Demonstration of safety of probiotics - A review
20. Rapid silver staining and recovery of PCR products separated on polyacrylamide gels
21. Bifidobacterial diversity in human feces detected by genus-specific polymerase chain reaction and denaturing gradient gel electrophoresis
22. Analysis of the fecal microflora of human subjects consuming a probiotic products containing Lactobacillus rhamnosus DR 20
23. Molecular approaches to study probiotic bacteria
24. A molecular view of the intestinal ecosystem
25. Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria