Liver-targeted gene transfer into a human hepatoblastoma cell line and in vivo by sterylglucoside-containing cationic liposomes

Gene Therapy

Volumn 8, Issue 16, 2001, Pages 1276-1280

  Hwang, S H ^a   Hayashi, K ^b   Takayama, K ^a   Maitani, Y ^a  

^a HOSHI UNIVERSITY   (Japan)

^b UNIVERSITY OF TOYAMA   (Japan)

Author keywords

Cationic liposome; DC Chol; Gene transfer; Liver target; Serum; Sterylglucoside; Tfx 20

Indexed keywords

3BETA[N(N',N' DIMETHYLAMINOETHANE)CARBAMOYL]CHOLESTEROL; ALCOHOL; ASIALOFETUIN; ASIALOGLYCOPROTEIN RECEPTOR; BOVINE SERUM ALBUMIN; CATION; CHOLESTEROL DERIVATIVE; DIOLEOYLPHOSPHATIDYLETHANOLAMINE; DNA; GALACTOSE; HEPARIN; IMMUNOGLOBULIN G; LACTOSYLCERAMIDE; LIGAND; LIPID; LIPOPROTEIN; LIPOSOME; LUCIFERASE; N,N,N',N' TETRAMETHYL N,N' BIS(2 HYDROXYETHYL) 2,3 DI(OLEOYLOXY) 1,4 BUTANEDIAMMONIUM IODIDE; PLASMID DNA; REAGENT; SITOGLUSIDE; SITOSTEROL DERIVATIVE; TFX 20; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CHEMICAL ANALYSIS; CHEMICAL COMPOSITION; COMPLEX FORMATION; CONTROLLED STUDY; CULTURE MEDIUM; DNA BINDING; DNA VECTOR; DRUG ACTIVITY; DRUG EFFICACY; DRUG FORMULATION; FEMALE; GENE EXPRESSION; GENE TARGETING; GENE TRANSFER; GENETIC TRANSFECTION; HEPATOBLASTOMA; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; LIVER; LIVER CELL CULTURE; MARKER GENE; MOUSE; NONHUMAN; PRIORITY JOURNAL; SERUM; TARGET ORGAN;

ANIMALS; CHOLESTEROL; GENE EXPRESSION; GENE THERAPY; GENETIC VECTORS; GLYCEROPHOSPHOLIPIDS; HEPATOBLASTOMA; HUMANS; INJECTIONS, INTRAVENOUS; LIPOSOMES; LIVER; LIVER NEOPLASMS, EXPERIMENTAL; LUCIFERASES; MICE; MICROSCOPY, ELECTRON, SCANNING; PHOSPHATIDYLETHANOLAMINES; SITOSTEROLS; TISSUE DISTRIBUTION; TRANSFECTION;

EID: 0034889026     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3301510     Document Type: Article

References (27)

1. Non-viral gene therapy
2. Gene therapy for cystic fibrosis: A clinical perspective
3. Receptor-mediated gene delivery and expression in vivo
4. Targeted gene transfer into hepatoma cells with lipopolyamine-condensed DNA particles presenting galactose ligands: A stage toward artificial viruses
5. Lactose-poly(ethylene glycol)-grafted poly-L-lysine as hepatoma cell-targeted gene carrier
6. Receptor-mediated transfer of pSV2CAT DNA to a human hepatoblastoma cell line HepG2 using asialofetuin-labeled cationic liposomes
7. Effects of fusogenic and DNA-binding amphiphilic compounds on the receptor-mediated gene transfer into hepatic cells by asialofetuin-labeled liposomes
8. Increased liver uptake of liposomes and improved targeting efficacy by labeling with asialofetuin in rodents
9. Evaluation of dipalmitoylphosphatidylcholine liposomes containing a soybean-derived sterylglucoside mixture for liver targeting
10. Formulation of liposomes with a soybean-derived sterylglucoside mixture and cholesterol for liver targeting
11. Targeting of soybean-derived sterylglucoside liposomes to liver tumors in rat and mouse models
12. Physicochemical characteristics and transfection efficiency of DNA in cationic and neutral liposomes with soybean-derived sterylglucoside into HepG2 cells
13. Reproducible and efficient murine CNS gene delivery using a microprocessor-controlled injector
14. A novel cationic liposome reagent for efficient transfection of mammalian cells
15. Direct gene transfer with DNA-liposome complexes in melanoma: Expression, biologic activity, and lack of toxicity in humans
16. Human serum albumin enhances DNA transfection by lipoplexes and confers resistance to inhibition by serum
17. Effect of serum components on the physico-chemical properties of cationic lipid/oligonucleotide complexes and on their interactions with cells
18. Efficiency of liposomes surface-modified with soybean-derived sterylglucoside for liver targeting carrier in HepG2 cells
19. Systemic gene expression after intravenous DNA delivery into adult mice
20. Improved DNA:liposome complexes for increased systemic delivery and gene expression
21. Characterization of cationic liposome-mediated gene transfer in vivo by intravenous administration
22. Stabilization of cationic liposome-plasmid DNA complexes by polyamine and poly(ethylene glycol)-phospholipid conjugates for efficient in vivo gene delivery
23. In vivo gene transfer via intravenous administration of cationic lipid-protamine-DNA (LPD) complexes
24. Pharmacokinetics, tissue distribution, and expression efficiency of plasmid 32P-DNA following intravenous administration of DNA/cationic lipid complexes in mice: Use of a novel radionuclide approach
25. In vivo gene delivery to the liver using novel galactosylated cationic liposomes
26. Modified ethanol injection method for liposomes containing β-sitosterol β-D-glucoside
27. New structures in complex formation between DNA and cationic liposomes visualized by freeze-fracture electron microscopy