Caution in the use of 2-iminothiolane (Traut's reagent) as a cross-linking agent for peptides. The formation of N-peptidyl-2-iminothiolanes with bombesin (BN) antagonist (D-Trp6,Leu13-ψ[CH2NH]-Phe14) BN6-14 and D-Trp-Gln-Trp-NH2

Journal of Peptide Research

Volumn 57, Issue 5, 2001, Pages 383-389

  Mokotoff, M ^a,b,c   Mocarski, Y M ^a,b,c   Gentsch, B L ^a,b,c   Miller, M ^a,b,c   Zhou, J H ^a,b,c   Chen, J ^a,b,c   Ball, E D ^a,b,c  

^a UNIVERSITY OF PITTSBURGH   (United States)

^b UNIVERSITY OF PITTSBURGH MEDICAL CENTER   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

2 iminothiolane; Bispecific molecules; Cross linking agent; N peptidyl 2 iminothiolanes; Thiol trapping with N methylmaleimide; Traut's reagent

Indexed keywords

2 IMINOTHIOLANE; BOMBESIN ANTAGONIST; CROSS LINKING REAGENT; MONOCLONAL ANTIBODY; PEPTIDE; THIOL GROUP; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL REACTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN STABILITY; REACTION TIME;

ANTIBODIES, MONOCLONAL; BOMBESIN; CROSS-LINKING REAGENTS; IMIDOESTERS; PEPTIDE FRAGMENTS; PEPTIDES;

STAPHYLOCOCCUS PHAGE 3A;

EID: 0034888423     PISSN: 1397002X     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-3011.2001.00845.x     Document Type: Article

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