Expression of the myc/his-tagged human peptide transporter hPEPT1 in yeast for protein purification and functional analysis

Protein Expression and Purification

Volumn 22, Issue 3, 2001, Pages 436-442

  Theis, Stephan ^a   Döring, Frank ^a   Daniel, Hannelore ^a  

^a TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Human intestinal peptide transporter; Myc epitope; Pichia pastoris; Polyhistidine tag

Indexed keywords

EPITOPE; GENE TAGGING; GENETIC TRANSFORMATION; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HUMAN GENE; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MEMBRANE PROTEIN; MOLECULAR WEIGHT; PROMOTER REGION; PROTEIN DETECTION; PROTEIN EXPRESSION;

PICHIA; PICHIA PASTORIS;

EID: 0034888064     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1455     Document Type: Article

References (22)

1. The oligopeptide transporter (Pept-1) in human intestine: Biology and function
2. Function and molecular structure of brush border membrane peptide/H+ symporters
3. +/peptide cotransporter: Cloning, functional expression and chromosomal localisation
4. +-coupled transport of chephalosporins via dipeptide carriers in rabbit intestinal brush-border membranes: Difference of transport characteristics between cefixime and cephradine
5. Oral absorption of beta-lactams by intestinal peptide transport proteins
6. Angiotensin-converting enzyme (ACE) inhibitor transport in human intestinal epithelial (Caco-2) cells
7. Transport characteristics of differently charged cephalosporin antibiotics in oocytes expressing the cloned intestinal peptide transporter PEPT1 and in human intestinal Caco-2 cells
8. Stereoselective uptake of beta-lactam antibiotics by the intestinal peptide transporter
9. Application of high-performance liquid chromatography to the purification of the putative intestinal peptide transporter
10. Purification by ceftibuten-affinity chromatography and the functional reconstitution of oligopeptide transporter(s) in rat intestinal brush-border membrane
11. Expression cloning of a cDNA from rabbit small intestine related to proton-coupled transport of peptides, beta-lactam antibiotics and ACE-inhibitors
12. Expression cloning of a mammalian proton-coupled oligopeptide transporter
13. Recognition of beta-lactam antibiotics by rat peptide transporters, PEPT1 and PEPT2, in LLC-PK1 cells
14. Expression and functional characterization of the mammalian intestinal peptide transporter PEPT1 in the methylotropic yeast Pichia pastoris
15. Use of the glyceraldehyde-3-phosphate dehydrogenase promoter for production of functional mammalian membrane transport proteins in the yeast Pichia pastoris
16. Functional analysis of a chimeric mammalian peptide transporter derived from the intestinal and renal isoforms
17. Delta-aminolevulinic acid transport by intestinal and renal peptide transporters and its physiological and clinical implications
18. +/peptide symporter (PEPT1), the uptake into Caco-2 cell monolayers and the transepithelial flux
19. Functional expression of bovine opsin in the methylotrophic yeast Pichia pastoris
20. Comparative biochemical and pharmacological characterization of the mouse 5HT5A 5-hydroxytryptamine receptor and the human beta2-adrenergic receptor produced in the methylotrophic yeast Pichia pastoris
21. Expression of functional mouse 5-HT5A serotonin receptor in the methylotrophic yeast Pichia pastoris: Pharmacological characterization and localization