Mutations in the Escherichia coli receptor FepA reveal residues involved in ligand binding and transport

Molecular Microbiology

Volumn 41, Issue 3, 2001, Pages 527-536

  Barnard, T J ^a   Watson M E , Jr ^a   McIntosh, M A ^a  

^a University of Missouri   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COLICIN; COLICIN B; COLICIN D; CYTOPLASM PROTEIN; MEMBRANE RECEPTOR; PROTEIN FEPA; PROTEIN TONB; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL MUTATION; BACTERIAL OUTER MEMBRANE; CELL MEMBRANE TRANSPORT; CRYSTAL STRUCTURE; ESCHERICHIA COLI; LIGAND BINDING; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

AMINO ACID MOTIFS; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; BINDING, COMPETITIVE; CARRIER PROTEINS; COLICINS; CONSERVED SEQUENCE; ENTEROBACTIN; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; KINETICS; LIGANDS; MEMBRANE PROTEINS; MODELS, BIOLOGICAL; MODELS, MOLECULAR; MUTATION; PROTEIN CONFORMATION; PROTEIN TRANSPORT; RECEPTORS, CELL SURFACE;

EID: 0034888050     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2001.02473.x     Document Type: Article

References (36)

1. Functional analysis of a C-terminally altered TonB protein of Escherichia coli
2. Molecular analysis of the Escherichia coli ferric enterobactin receptor FepA
3. The Escherichia coli enterobactin biosynthesis gene, entD: Nucleotide sequence and membrane localization of its protein product
4. Genetic suppression demonstrates interaction of TonB protein with outer membrane transport proteins in Escherichia coli
5. The beta-barrel domain of FhuADelta5-160 is sufficient for TonB-dependent FhuA activities of Escherichia coli
6. Overexpression and purification of ferric enterobactin esterase from Escherichia coli. Demonstration of enzymatic hydrolysis of enterobactin and its iron complex
7. Molecular characterization of the Enterobacter aerogenes tonB gene: Identification of a novel type of tonB box suppressor mutant
8. Crystal structure of the outer membrane active transporter FepA from Escherichia coli
9. Formation of ion channels by colicin B in planar lipid bilayers
10. Sequence changes in the TonB box region of BtuB affect its transport activities and interaction with TonB protein
11. Site-directed disulfide bonding reveals an interaction site between energy-coupling protein TonB and BtuB, the outer membrane cobalamin transporter
12. Aromatic components of two ferric enterobactin binding sites in Escherichia coli FepA
13. Recognition and transport of ferric enterobactin in Escherichia coli
14. Siderophore-mediated iron transport: Crystal structure of FhuA with bound lipopolysaccharide
15. Point mutations in a conserved region (TonB box) of Escherichia coli outer membrane protein BtuB affect vitamin B12 transport
16. SWISS-MODEL and the Swiss-PdbViewer: An environment for comparative protein modeling
17. Suppression of the btuB451 mutation by mutations in the tonB gene suggests a direct interaction between TonB and TonB-dependent receptor proteins in the outer membrane of Escherichia coli
18. Vitamin B12 transport in Escherichia coli: Energy coupling between membranes
19. Mutual inhibition of cobalamin and siderophore uptake systems suggests their competition for TonB function
20. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
21. Regions of Escherichia coli TonB and FepA proteins essential for in vivo physical interactions
22. Protonmotive force, ExbB and ligand-bound FepA drive conformational changes in TonB
23. Transmembrane signaling across the ligand-gated FhuA receptor: Crystal structures of free and ferrichrome-bound states reveal allosteric changes
24. Genetic and physiological studies on the relationship between colicin B resistance and ferrienterochelin uptake in Escherichia coli K-12
25. Substrate-induced exposure of an energy-coupling motif of a membrane transporter
26. Surface topology of the Escherichia coli K-12 ferric enterobactin receptor
27. Double mutagenesis of a positive charge cluster in the ligand-binding site of the ferric enterobactin receptor, FepA
28. Aerobic regulation of the Escherichia coli tonB gene by changes in iron availability and the fur locus
29. TonB and the gram-negative dilemma
30. Identification of the Escherichia coli tonB gene product in minicells containing tonB hybrid plasmids
31. Characterization of group B colicin-resistant mutants of Escherichia coli K-12: Colicin resistance and the role of enterochelin
32. Uptake of ferrienterochelin by Escherichia coli: Energy dependent stage of uptake
33. Analysis of Escherichia coli TonB membrane topology by use of PhoA fusions
34. Transport across the outer membrane of Escherichia coli K12 via the FhuA receptor is regulated by the TonB protein of the cytoplasmic membrane
35. Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase