Synthesis, conformational studies and biological activity of Nα-mono-biotinylated rat relaxin

Journal of Peptide Research

Volumn 57, Issue 5, 2001, Pages 374-382

  Mathieu, M N ^a,b,c,d   Wade, J D ^a,b,c,d   Tregear, G W ^a,b,c,d   Bond, C P ^a,b,c,d   Summers, R J ^a,b,c,d   Catimel, B ^a,b,c,d   Nice, E C ^a,b,c,d   Otvos Jr , L ^a,b,c,d  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b MONASH UNIVERSITY   (Australia)

^c ROYAL MELBOURNE HOSPITAL   (Australia)

^d WISTAR INSTITUTE   (United States)

Author keywords

BIAcore biosensor; Biotinylated relaxin; Peptide aggregation; Peptide synthesis; Polyclonal antibody; Protein modification; Structure activity relationship

Indexed keywords

HORMONE RECEPTOR; POLYCLONAL ANTIBODY; RELAXIN;

ALPHA CHAIN; ANIMAL TISSUE; ARTICLE; BETA CHAIN; BIOASSAY; BIOSENSOR; BIOTINYLATION; CIRCULAR DICHROISM; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ASSEMBLY; PROTEIN CONFORMATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; RAT; RECEPTOR BINDING; STRUCTURE ACTIVITY RELATION;

ANIMALS; BIOSENSING TECHNIQUES; BIOTIN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; PROTEIN CONFORMATION; RATS; RELAXIN; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ANIMALIA;

EID: 0034881529     PISSN: 1397002X     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-3011.2001.00853.x     Document Type: Article

References (31)

1. X-Ray structure of human relaxin at 1-5 Å. Comparison to insulin and implications for receptor binding determinants
2. Relaxin
3. Cardiac effects of relaxin in rats
4. Relaxin alters the plasma osmolality arginine vasopressin relationship in the rat
5. The chemical synthesis of rat relaxin and the unexpectedly high potency of the synthetic hormone in the mouse
6. Relaxin
7. Strategies for the identification and purification of ligands for orphan biomolecules
8. Instrumental biosensors: New perspectives for the analysis of biomolecular interactions
9. Identification of specific relaxin-binding cells in the cervix, mammary glands, nipples, small intestine, and skin of pregnant pigs
10. Monobiotinylated relaxins
11. The receptor-binding site of human relaxin II
12. A technique for rapid purification of low yield products: Biotinylation of chemically synthesized proteins on-resin
13. Comparison of relaxin receptors in rat isolated atria and uterus by use of synthetic and native relaxin analogues
14. Reversible modification of the acid labile 2-hydroxy-4-methoxybenzyl (Hmb) amide protecting group: A simple scheme yielding backbone substituted free peptides
15. Primate relaxin: Synthesis of gorilla and rhesus monkey relaxins
16. Synergies between micropreparative high-performance liquid chromatography and an instrumental optical biosensor
17. Micropreparative LC of proteins
18. Purification and fragmentation of antibodies
19. Solid phase synthesis and biological activity of rat relaxin
20. Some 'difficult sequences' made easy
21. The synthesis of 'difficult' peptides using 2-hydroxy-4-methoxybenzyl or pseudoproline amino acid building blocks: A comparative study
22. Pseudoprolines as solubilizing, structure-disrupting protection technique in peptide synthesis
23. Use of the Hmb backbone-protecting group in the synthesis of difficult sequences
24. Use of Fmoc-(2-hydroxy-4-methoxybenzyl) amino acids in peptide synthesis
25. Conformational effects in the reversed-phase liquid chromatography of ribonuclease A
26. Circular dichroism studies of relaxin and insulin peptide chains
27. Occurrence and minimization of cysteine racemisation during stepwise solid-phase peptide synthesis
28. 3-(1-Piperidinyl) alanine formation during the preparation of C-terminal cysteine peptides with the Fmoc/t-Bu strategy
29. Improved preparation of β-amyloid (1-43): Structural insight leading to optimised positioning of N-(2-hydroxy-4-methoxybenzyl) (Hmb) backbone amide protection
30. Solid-phase synthesis and biological activity of rat relaxin