Bullous pemphigoid sera react specifically with various domains of BP230, most frequently with C-terminal domain, by immunoblot analyses using bacterial recombinant proteins covering the entire molecule

Experimental Dermatology

Volumn 10, Issue 4, 2001, Pages 256-263

  Hamada, T ^a   Nagata, Y ^a   Tomita, M ^b   Salmhofer, W ^a,c   Hashimoto, Takashi ^a  

^a KURUME UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b KITASATO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^c UNIVERSITY OF GRAZ   (Austria)

Author keywords

BP230; Bullous pemphigoid; Epitope; Pemphigus; Recombinant protein

Indexed keywords

COMPLEMENTARY DNA; GLUTATHIONE TRANSFERASE; PEMPHIGUS ANTIBODY; RECOMBINANT PROTEIN; TISSUE EXTRACT; VIRUS FUSION PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DNA LIBRARY; EPIDERMIS; EXPRESSION VECTOR; EXTRACTION; HUMAN; HUMAN TISSUE; IMMUNOBLOTTING; KERATINOCYTE; MOLECULE; NONHUMAN; PEMPHIGOID; PEMPHIGUS; PEMPHIGUS FOLIACEUS; PEMPHIGUS VULGARIS; POLYMERASE CHAIN REACTION; PROTEIN DOMAIN; SERUM;

ANTIBODIES, MONOCLONAL; AUTOANTIGENS; AUTOIMMUNE DISEASES; BACTERIAL PROTEINS; BLOOD; CARRIER PROTEINS; COLLAGEN; CYTOSKELETAL PROTEINS; HUMANS; IMMUNOBLOTTING; NERVE TISSUE PROTEINS; NON-FIBRILLAR COLLAGENS; PEMPHIGOID, BULLOUS; RECOMBINANT FUSION PROTEINS; SKIN; SKIN DISEASES, VESICULOBULLOUS;

EID: 0034863878     PISSN: 09066705     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-0625.2001.100405.x     Document Type: Article

References (35)

1. Pemphigus and pemphigoid as paradigm of organ-specific, autoantibody-mediated diseases
2. Bullous pemphigoid, cicatricial pemphigoid, and chronic bullous disease of childhood
3. Skin diseases related to abnormality in desmosomes and hemidesmosomes
4. Characterization of bullous pemphigoid antigen: A unique basement membrane protein of stratified squamous epithelia
5. Isolation of complementary DNA for bullous pemphigoid antigen by use of patients' autoantibodies
6. Production of human monoclonal anti-basement membrane zone (BMZ) antibodies from a patient with bullous pemphigoid (BP) by Epstein-Barr virus transformation. Analyses of the heterogeneity of anti-BMZ antibodies in BP sera using them
7. Comparison of molecularly cloned bullous pemphigoid antigen to desmoplakin I confirms that they define a new family of cell adhesion junction plaque proteins
8. Human bullous pemphigoid antigen (BPAG1). Amino acid sequences deduced from cloned cDNAs predict biologically important peptide segments and protein domains
9. Further analyses of epitopes for human monoclonal anti-basement membrane zone antibodies produced by stable human hybridoma cell lines constructed with Epstein-Barr virus transformants
10. Molecular heterogeneity of the bullous pemphigoid antigens as detected by immunoblotting
11. Isolation of a human epidermal cDNA corresponding to the 180-kD autoantigen recognized by bullous pemphigoid and herpes gestationis sera
12. Cloning and primary structural analysis of the bullous pemphigoid autoantigen BP180
13. Bullous pemphigoid and herpes gestationis autoantibodies recognize a common non-collagenous site on the BP180 ectodomain
14. The extracellular domain of BPAG2 localizes to anchoring filaments and its carboxyl terminus extends to the lamina densa of normal human epidermal basement membrane
15. Production of the entire extracellular domain of BP180 (type VII collagen) by baculovirus expression
16. Comparative structural analysis of desmoplakin, BP antigen and plectin: Members of a new gene family involved in organization of intermediate filaments
17. The plakin family: Versatile organizers of cytoskeletal architecture
18. Cicatricial pemphigoid autoantibodies react with multiple sites on the BP180 extracellular domain
19. Majority of bullous pemphigoid and herpes gestationis sera react with NC16a domain of the 180 kD bullous pemphigoid antigen
20. Tight clustering of extracellular BP180 epitopes recognized by bullous pempphigoid autoantibodies
21. Bullous pemphigoid sera that contain antibodies to BPAg2 also contain antibodies to LABD97 that recognize epitopes distal to the NC16A domain
22. A passive transfer model of the organ-specific autoimmune disease, bullous pemphigoid, using antibodies generated against the hemidesmosomal antigen, BP180
23. Molecular mapping of pathogenically relevant BP180 epitope associated with experimentally induced murine bullous pemphigoid
24. IgG antibodies from patients with bullous pemphigoid bind to localized epitopes on synthetic peptides encoded by bullous pemphigoid antigen cDNA
25. Characterization of BP antibodies by use of recombinant BP antigen proteins
26. Detection of autoantibodies against bullous pemphigoid and pemphigus antigens by an enzyme-linked immunosorbent assay using the bacterial recombinant proteins
27. Comparative epitope mapping of sera from United States (US) and Japanese patients with bullous pemphigoid (BP) to fusion proteins encoded by BPAG1
28. IgG autoantibodies from bullous pemphigoid patients recognize multiple antigenic reactive sites located predominantly within the B and C subdomains of the COOH-terminus of BP230
29. Detection of pemphigus vulgaris and pemphigus foliaceus antigens by immunoblot analysis using different antigen sources
30. Comparison study of bullous pemphigoid antigens among Japanese, British, and US patients indicates present both in the basement membrane and on the keratinocyte cell membrane
31. Clinical manifestations in 100 Japanese bullous pemphigoid cases in relation to autoantigen profiles
32. Epitope spreading
33. Epitope spreading: Lessons from autoimmune skin diseases
34. Characterization of paraneoplastic pemphigus autoantigens by immunoblot analysis
35. A 230-kD basic protein is the major bullous pemphigoid antigen