ATP induces Ca2+ signaling in human chondrons cultured in three-dimensional agarose films

Osteoarthritis and Cartilage

Volumn 9, Issue 6, 2001, Pages 518-526

  Elfervig, M K ^a,b   Graff, R D ^b   Lee, G M ^b   Kelley, S S ^b   Sood, A ^b   Banes, A J ^a,b  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

ATP; Chondrocytes; Intracellular Calcium; Signal Transduction

Indexed keywords

2 METHYLTHIOADENOSINE TRIPHOSPHATE; ADENOSINE 5' O (3 THIOTRIPHOSPHATE); ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; AGAROSE; CALCIUM ION; FURA 2 ACETOXYMETHYL ESTER; NITRIC OXIDE; NUCLEOSIDE; NUCLEOTIDE; PURINE RECEPTOR; PURINERGIC RECEPTOR BLOCKING AGENT; SURAMIN; URIDINE TRIPHOSPHATE;

ARTICLE; CALCIUM CELL LEVEL; CALCIUM SIGNALING; CARTILAGE CELL; CASE REPORT; CELL COMMUNICATION; CELL CONTACT; CELL CULTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE MEDIUM; EXTRACELLULAR MATRIX; FILM; FLUORESCENCE; GAP JUNCTION; GEL; HUMAN; HUMAN CELL; HYPOTHESIS; IN VIVO CULTURE; INCUBATION TIME; OSTEOARTHRITIS; PRIORITY JOURNAL; REFERENCE VALUE; SIGNAL TRANSDUCTION; THREE DIMENSIONAL IMAGING;

EID: 0034855731     PISSN: 10634584     EISSN: None     Source Type: Journal    
DOI: 10.1053/joca.2000.0435     Document Type: Article

References (35)

1. Athletics and osteoarthritis
2. Chondrons from articular cartilage. (V) Immunohistochemical evaluation of type VI collagen organisation in isolated chondrons by light, confocal and electron microscopy
3. Articular cartilage chondrons: Form, function and failure
4. ATP release by mechanically loaded chondrons in pellet culture
5. The effects of static and dynamic compression on nitric oxide production in articular cartilage explants
6. The role of cartilage streaming potential, fluid flow and pressure in the stimulation of chondrocyte biosynthesis during dynamic compression
7. Nitric oxide and G proteins mediate the response of bovine articular chondrocytes to fluid-induced shear
8. 2+ mobilization in articular chondrocytes
9. Effects of fluid flow on intracellular calcium in bovine articular chondrocytes
10. 2+ signaling in human tendon epitenon cells
11. Human tendon cells express connexin-43 and propagate a calcium wave in response to mechanical stimulation
12. Intercellular calcium response of ACL and MCL ligament fibroblasts to fluid-induced shear stress
13. ATP transiently blocks mechanical load responsiveness in human annulus cells
14. Mechanism of mechanically induced intercellular calcium waves in rabbit articular chondrocytes and in HIG-82 synovial cells
15. 2+ signaling in articular chondrocytes
16. 2+ waves in ATP-stimulated articular chondrocytes
17. Calcium-mobilizing purine receptors on the surface of mammalian articular chondrocytes
18. 2 release
19. 2-purinoceptors
20. Involvement of distinct receptors in the actions of extracellular uridine nucleotides
21. Signal transduction via P2-purinergic receptors for extracellular ATP and other nucleotides
22. Mechanisms and function of intercellular calcium signaling
23. 2U purinoceptors: cDNA cloning, signal transduction mechanisms and structure-function analysis
24. ATP and UTP activate calcium-mobilizing P2U-like receptors and act synergistically with interleukin-1 to stimulate prostaglandin E2 release from human rheumatoid synovial cells
25. Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation
26. i and enhance basic fibroblast growth factor-induced proliferation in sheep chondrocytes
27. Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads
28. Isolated chondrons: A viable alternative for studies of chondrocyte metabolism in vitro
29. Practical design criteria for a dynamic ratio imaging system
30. Intercellular calcium signaling between chondrocytes and synovial cells in co-culture
31. Specificity of a porcine 127-kd nucleotide pyrophosphohydrolase for articular tissues