Long-term effects of osteogenic protein-1 on biosynthesis and proliferation of human articular chondrocytes

Clinical and Experimental Rheumatology

Volumn 19, Issue 5, 2001, Pages 525-531

  Huch, K ^a  

^a UNIVERSITY OF ULM   (Germany)

Author keywords

Biosynthesis; Collagen; Human articular chondrocytes; Long term culture; Proteoglycan

Indexed keywords

AGGRECAN; ALGINIC ACID; COLLAGEN; DNA; HYDROXYPROLINE; METHYLENE BLUE; OSTEOGENIC PROTEIN 1; PROLINE; PROTEOGLYCAN; SULFATE; SULFUR 35; TRITIUM;

ADULT; AGE; ARTICLE; ARTICULAR CARTILAGE; ASSAY; BIOENGINEERING; BIOSYNTHESIS; CARTILAGE CELL; CARTILAGE MATRIX; CELL CULTURE; CELL PROLIFERATION; CELL STIMULATION; COLLAGEN SYNTHESIS; COLORIMETRY; COMPARATIVE STUDY; CONTROLLED STUDY; DNA CONTENT; EXPOSURE; FLUOROMETRY; HUMAN; HUMAN CELL; HUMAN TISSUE; ISOTOPE LABELING; MEASUREMENT; ORGAN DONOR; PHENOTYPE; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN SYNTHESIS; PROTEOGLYCAN SYNTHESIS;

ADULT; AGED; AGGRECANS; BONE MORPHOGENETIC PROTEINS; CARTILAGE, ARTICULAR; CELL DIVISION; CELLS, CULTURED; CHILD, PRESCHOOL; CHONDROCYTES; COLLAGEN; DNA; DOSE-RESPONSE RELATIONSHIP, DRUG; EXTRACELLULAR MATRIX PROTEINS; FETUS; HUMANS; INFANT; INFANT, NEWBORN; LECTINS, C-TYPE; MIDDLE AGED; PROTEOGLYCANS; TRANSFORMING GROWTH FACTOR BETA;

EID: 0034837758     PISSN: 0392856X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (22)

1. Bone morphogenetic proteins (BMP-2 and BMP-3) promote growth and expression of the differentiated phenotype of rabbit chondrocytes and osteoblastic MC3T3-E1 cells in vitro
2. Stimulation of the expression of osteogenic and chondrogenic phenotypes in vitro by osteogenin
3. Natural bovine osteogenin and recombinant human bone morphogenetic protein-2B are equipotent in the maintenance of proteoglycans in bovine articular cartilage explant cultures
4. Bovine articular chondrocytes do not undergo hypertrophy when cultured in the presence of serum and osteogenic protein-1
5. Recombinant bone morphogenetic protein-4, transforming growth factor-β1, and activin A enhance the cartilage phenotype of articular chondrocytes in vitro
6. Recombinant human osteogenic protein 1 is a potent stimulator of the synthesis of cartilage proteoglycans and collagens by human articular chondrocytes
7. Effects of recombinant human osteogenic protein 1 on the production of proteoglycan, prostaglandin E2, and interleukin-1 receptor antagonist by human articular chondrocytes cultured in the presence of interleukin-1β
8. Osteogenic protein 1 stimulates cell-associated matrix assembly by normal human articular chondrocytes. Up-regulation of hyaluron synthase, CD 44, and aggrecan
9. Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads
10. Stimulation of proteoglycan synthesis in explants of porcine articular cartilage by recombinant osteogenic protein-1 (bone morphogenetic protein-7)
11. Recombinant human bone morphogenetic protein-2 maintains the articular chondrocyte phenotype in long-term culture
12. Differences between subpopulations of cultured bovine articular chondrocytes. I. Morphology and cartilage matrix production
13. Synthesis and turnover of proteoglycans by human and bovine adult articular chondrocytes cultured in alginate beads
14. Recombinant human Osteogenic Protein-1 (hOP-1) induces new bone formation in vivo with specific activity comparable with natural bovine Osteogenic Protein and stimulates osteoblast proliferation and differentiation in vitro
15. 35S-labeled proteoglycans complexed to alcian blue by rapid filtration in multiwell plates
16. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
17. A novel proteoglycan synthesized and secreted by chondrocytes of the superficial zone of articular cartilage
18. Microdetermination of proteoglycans and glycosaminoglycans in the presence of guanidine hydrochloride
19. Quantitative analysis of collagen, protein and DNA in fixed, paraffin-embedded and sectioned tissue
20. Fluorometric assay of DNA in cartilage explants using Hoechst 33258
21. Human articular chondrocytes express osteogenic protein-1
22. Cartilage repair in osteoarthritis: The role of dermatan sulfate proteoglycans