Quantitative detection of Streptococcus pneumoniae in nasopharyngeal secretions by real-time PCR

Journal of Clinical Microbiology

Volumn 39, Issue 9, 2001, Pages 3129-3134

  Greiner, O ^a   Day, P J R ^a   Bosshard, P P ^a   Imeri, F ^a   Altwegg, M ^a   Nadal, D ^a  

^a UNIVERSITY CHILDREN S HOSPITAL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; PNEUMOLYSIN;

ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; GENE; HUMAN; NASOPHARYNX; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESPIRATORY TRACT INFECTION; STREPTOCOCCUS INFECTION; STREPTOCOCCUS PNEUMONIAE;

BACTERIAL PROTEINS; CHILD; CULTURE MEDIA; DNA PRIMERS; DNA, BACTERIAL; HUMANS; NASOPHARYNX; PNEUMOCOCCAL INFECTIONS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STREPTOCOCCUS PNEUMONIAE; STREPTOLYSINS;

BACTERIA (MICROORGANISMS); HAEMOPHILUS INFLUENZAE; MORAXELLA CATARRHALIS; POSIBACTERIA; STREPTOCOCCUS PNEUMONIAE;

EID: 0034837298     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.39.9.3129-3134.2001     Document Type: Article

References (21)

1. Clinical utility of blood cultures in adult patients with community-acquired pneumonia without defined underlying risks
2. Comparison of the ABI 7700 system (TaqMan) and competitive PCR for quantification of IS6110 DNA in sputum during treatment of tuberculosis
3. Community acquired pneumonia - A prospective UK study
4. Development and evaluation of a broad-range PCR-ELISA assay with Borrelia burgdorferi and Streptococcus pneumoniae as model organisms for reactive arthritis and bacterial meningitis
5. Septic arthritis
6. Etiology of childhood pneumonia: Serologic results of a prospective, population-based study
7. Etiology of community-acquired pneumonia in 254 hospitalized children
8. Optochin resistance in Streptococcus pneumoaiae strains isolated from blood and middle ear fluid
9. Diagnosis of pneumococcal pneumonia by polymerase chain reaction (PCR) in whole blood: A prospective clinical study
10. Quantitative real-time PCR for Porphyromonas gingivalis and total bacteria
11. Confirmation of psaA in all 90 serotypes of Streptococcus pneumoniae by PCR and potential of this assay for identification and diagnosis
12. Ambiguity in the identification of Streptococcus pneumoniae. Optochin, bile solubility, quellung, and the AccuProbe DNA probe tests
13. Community-acquired pneumonia in children due to Mycoplasma pneumoniae: Diagnostic performance of a seminested 16S rDNA-PCR
14. The causes of hospital-treated acute lower respiratory tract infection in children
15. Quantitative detection of Borrelia burgdorferi by real-time PCR
16. Natural development of antibodies to pneumococcal surface protein A, pneumococcal surface adhesin A, and pneumolysin in relation to pneumococcal carriage and acute otitis media
17. Bacterial meningitis in the United States in 1995
18. Characterisation of the gene encoding suilysin from Streptococcus suis and expression in field strains
19. Molecular cloning, characterization, and complete nucleotide sequence of the gene for pneumolysin, the sulfhydryl-activated toxin of Streptococcus pneumoniae
20. Genetic relationships between clinical isolates of Streptococcus pneumoniae, Streptococcus oralis, and Streptococcus mitis: Characterization of "atypical" pneumococci and organisms allied to S. mitis harboring S. pneumoniae virulence factor-encoding genes