Characterization of extracellular polymeric substances in rotating biological contactors and activated sludge flocs

Environmental Technology (United Kingdom)

Volumn 22, Issue 8, 2001, Pages 951-959

  Martin Cereceda M ^a   Jorand, F ^b   Guinea, A ^a   Block, J C ^b  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b CNRS   (France)

Author keywords

Activated sludges; Biofilms; Exopolymers; Rotating biological contactors

Indexed keywords

BIOLOGICAL WATER TREATMENT; ION EXCHANGE RESINS; POLYSACCHARIDES; PROTEINS; WASTEWATER TREATMENT;

ACTIVATED SLUDGE; ACTIVATED SLUDGE FLOCS; CATION EXCHANGE RESINS; EXOPOLYMERS; EXTRACELLULAR POLYMERIC SUBSTANCES; ROTATING BIOLOGICAL CONTACTOR; ROTATING BIOLOGICAL CONTRACTOR; WASTEWATER TREATMENT SYSTEM;

BIOFILMS;

CATION EXCHANGE RESIN; DNA; POLYMER; POLYSACCHARIDE; PROTEIN;

ACTIVATED SLUDGE; BIOFILM; EFFLUENT TREATMENT; POLYMER;

ACTIVATED SLUDGE; ARTICLE; BIOFILM; CHEMICAL COMPOSITION; CONTROLLED STUDY; EXTRACTION; HYDROPHOBICITY; MICROBIAL GROWTH; ROTATION; ULTRASOUND; WASTE WATER MANAGEMENT;

EID: 0034834650     PISSN: 09593330     EISSN: 1479487X     Source Type: Journal    
DOI: 10.1080/09593332208618231     Document Type: Article

References (39)

1. Jorand, F., Zartanan, F., Thomas, F., Block, J. C., Bottoro, J. Y., Villemin, G., Urbain, V. And Manem, J., Chemical and structural (2D) linkage between bacteria within activated sludge floes. Water Res., 29, 1639-1647 (1995).
2. Frolund, B., Palmgren, R., Keiding, K. And Nielsen, P. H., Extraction of extracellular polymers from activated sludge using a cation exchange resin. Water Res., 30, 1749-1758 (1996).
3. Jahn, A. And Nielsen, P. H., Extraction of extracellular polymeric substances (EPS) from biofilms using a cation exchange resin. Water Sci. Technol., 32, 157-164 (1995).
4. Charackiis, W. G. And Marshall, K. C., Biofilms: a basis for an interdisciplinary approach. In: Biofilms, Characklis, W.-G. And Marshall, K.-C. (eds.), John Wiley and Sons, New York, pp. 3-52 (1990).
5. Neu, T. R., The challenge to analyse extracellular polymers in biofilms. In: Microbial Mats, Structure, Deivlopment and Environmental Significance, Stal L. J. And Caumette P. (eds.), NATO ASI Series, vol. 35, Springer, Berlin, pp. 221-227 (1994).
6. Horan, N. J. And Eccles, C. R., Purification and characterization of extracellular polysaccharide from activated sludges. Water Res., 20, 1427-1432 (1986).
7. Bruus, J. H., Nielsen, P. H. And Keiding, K., On the stability of activated sludge floes with implications to dewatering. Water Res., 26, 1597-1604 (1992).
8. Urbain, V., Block, J. C. And Manem, J., Bioflocculation in activated sludge: an analytical approach. Water Res., 27, 829-838 (1993).
9. Pavoni, J. L., Tenney, M. W. And Echelberger, W. F., Bacterial exocellular polymers and biological flocculation. J. Water Pollut. Control Fed., 44, 414-431 (1972).
10. Eriksson, L. And Aim, B., Study of flocculation mechanisms by observing effects of a complexing agent on activated sludge properties. Water Sci. Technol., 24, 21-28 (1991).
11. Zita, A. And Hermansson, M., Effects of bacterial cell surface structure and hydrophobicity on attachment to activated sludge floes. Appl. Environ. Microbiol., 63, 1168-1170 (1997).
12. Wolfaardt, G. M., Lawrence, J. R., Robarts, R. D., Headley, J. V. And Caldwell, D. E., Microbial exopolymers provide a mechanism for bioaccumulation of contaminants. Microbiol. Ecol., 27, 279-291 (1994).
13. Neu, T. R. And Lawrence, J. R., Development and structure of microbial biofilms in river water studied by confocal laser scanning microscopy. FEMS Microbiol. Ecol., 24, 11-25 (1997).
14. Spath, R., Flemming, H. C. And Wuertz, S., Sorption properties of biofilms. Water Sci. Technol., 37, 207-210 (1998).
15. Zhang, X., Bishop, P. L. And Kupferle, M. J., Measurement of polysaccharides and proteins in biofilm extracellular polymers. Water Sci. Technol., 37, 345-348 (1998).
16. Brown, M. J. And Lester, J. N., Comparison of bacterial exocellular polymer extraction methods. Appl. Environ. Microbiol., 40, 179-185 (1980).
17. Goodwin, J. A. S. And Forster, C. F., A further examination into the composition of activated sludge surfaces in relation to their settlement characteristics. Water Res., 19, 527-533 (1985).
18. Frelund, B., Griebe, T. And Nielsen, P. H., Enzymatic activity in the activated sludge floe matrix. Appl. Microbiol. Biotechnol., 43, 755-761 (1995).
19. Bura, R., Cheung, M., Finlayson, J., Lee, B. C., Droppo, I. G., Leppard, G. G. And Liss, S. N., Composition of extracellular polymeric substances in the activated sludge floe matrix. Water Sci. Technol., 37, 325-333 (1998).
20. Dignac, M. F., Urbain V., Rybacki, D., Bruchet, A., Snidaro, D. And Scribe, P., Chemical description of extracellular polymers: implication on activated sludge floe structure. Water Sci. Technol., 38, 45-53 (1998).
21. Jorand, F., Boué-Bigne, F., Block, J. C. And Urbain, V., Hydrophobic/hydrophilic properties of activated sludge exopolymeric substances. Water Sci. Technol., 37, 307-315 (1998).
22. Azeredo, J., Lazarova, V. And Oliveira, R., Methods to extract the exopolymeric matrix from biofilms: a comparative study. Water Sci. Technol., 39, 243-250 (1999).
23. Curds, C. R., Interactions protozoaires-bactéries dans les méthodes aérobies de traitement des eaux usées L’Ann. Biolog., 32, 1-12 (1993).
24. Martin-Cereceda, M., Characterisation of a RBC wastewater treatment system. Biofilm analysis. PhD Thesis. Facultad de Biologia, Universidad Complutense de Madrid (2000).
25. Martin-Cereceda, M., Serrano, S. And Guinea, A., Biofilm communities and operational monitoring of a rotating biological contactor system. Water, Air and Soil Pollut., 126, 193-206 (2001).
26. Pérez-Uz, B., (unpublished results).
27. APHA, AWWA and WPCF, Standard Methods for the Examination of Water and Wastnoater, 19 ed. American Public Health Association/American Works Association/Water Environment Federation, Washington DC (1995).
28. Lowry, O. H., Rosebourgh, N. J., Farr, A. L. And Randall R. J., Proteins measurement with the folin phenol reagent. J. Biol. Chem., 193, 265-275 (1951).
29. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. And Smith, F., Colorimetric method for determination of sugars and related substances. Anal. Chem., 28, 350-356 (1956).
30. Blumenkrantz, N. And Asboe-Hansen, G., New method for quantitative determination of uronic acids. Anal. Biochem., 54, 484-489 (1973).
31. Deriaz, R. E., Stacey, M., Teece, E. G. And Wiggins, L. F., Deoxy-sugars. Part I. The Dische reaction for 2-deox y pentoses. J. Chem. Soc., 14, 1222-1232 (1949).
32. Lindahl, M., Faris, A., Wadstrcim, T. And Hjertén, S., A new test on ‘salting out’ to measure relative surface hydrophobicity of bacterial cells. Biochi. Biophys. Acta, 677, 471-476 (1981).
33. Danielsson, A., Norkans, B. And Bjomsson, A., On bacterial adhesion-the effect of certain enzymes on adhered cells of a marine Pseudomonas sp. Bot. Mar. 20, 13-17 (1977).
34. Jahn, A. And Nielsen, P. H., Cell biomass and exopolymer composition in sewer biofilms. Water Sci. Technol., 37, 17-24 (1998).
35. Glee, P.M. And Hazen, K.C., Aggregation of hydrophobic cell wall proteins of Candida albicans. Coll. And Surf., 5, 181-188 (1995).
36. Kocianova E., Foot, R. J. And Forster, C. F., Physicochemical aspects of activated sludge in relation to stable foam formation. J. Inst. Water Environ. Manage., 6, 342-350 (1992).
37. Allison, D.G. And Sutherland, I.W., The role of exopolysaccharides in adhesion of freshwater bacteria. J. Gen. Microbiol., 133, 1319-1327 (1987)
38. Vandevivere, P. And Kirchman, D. L., Attachment stimulates exopolysaccharide synthesis by a bacterium. Appl. Environ. Microbiol., 59, 3280-3286 (1993).
39. Ascon-Cabrera, M.A., Thomas, D. And Lebeault, J.M., Activity of synchronized cells of a steady-state biofilm recirculated reactor during xenobiotic biodégradation. Appl. Em’ircm. Microbiol., 61, 920-925 (1995).