Probing the role of oligomerization in the high thermal stability of Pyrococcus furiosus ornithine carbamoyltransferase by site-specific mutants

European Journal of Biochemistry

Volumn 268, Issue 14, 2001, Pages 3937-3942

  Clantin, Bernard ^a   Tricot, Catherine ^b   Lonhienne, Thierry ^c   Stalon, Victor ^a,b   Villeret, Vincent ^b  

^a UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

^b Institut de Recherches Microbiologiques J M Wiame   (Belgium)

^c UNIVERSITY OF LIÈGE   (Belgium)

Author keywords

Carbamoyltransferase; Extremophiles; Oligomerization; Ornithine

Indexed keywords

ENZYME VARIANT; GLUTAMIC ACID; METHIONINE; MUTANT PROTEIN; ORNITHINE CARBAMOYLTRANSFERASE; TRYPTOPHAN;

ARTICLE; CATALYSIS; DISSOCIATION; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STABILITY; HIGH TEMPERATURE; MOLECULAR INTERACTION; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN QUATERNARY STRUCTURE; PYROCOCCUS FURIOSUS; REACTION ANALYSIS; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS;

ENZYME STABILITY; HEAT; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; ORNITHINE CARBAMOYLTRANSFERASE; PROTEIN DENATURATION; PROTEIN STRUCTURE, QUATERNARY; PYROCOCCUS FURIOSUS;

EID: 0034822181     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.02302.x     Document Type: Article

References (33)

1. Structure and function of ornithine carbamoyltransferases
2. Biosynthesis and metabolism of arginine in bacteria
3. Evolutionary relationships among bacterial carbamoyltransferases
4. Pyrococcus furiosus sp. nov. represents a novel genus of marine heterotrophic archaebacteria growing optimally at 100°C
5. Biochemical characterisation of ornithine carbamoyltransferase from Pyrococcus furiosus
6. The crystal structure of citrate synthase from the thermophilic archaeon, Thermoplasma acidophilum
7. Structure of a hyperthermophilic tungstopterin enzyme, aldehyde ferredoxin oxidoreductase
8. Crystal structure of recombinant triosephosphate isomerase from Bacillus stearothermophilus. An analysis of potential thermostability factors in six isomerases with known three-dimensional structures points to the importance of hydrophobic interactions
9. Crystal structure of the large fragment of Thermus aquaticus DNA polymerase I at 2.5-A resolution: Structural basis for thermostability
10. Aromatic-aromatic interaction: A mechanism of protein structure stabilization
11. An additional aromatic interaction improves the thermostability and thermophilicity of a mesophilic family 11 xylanase: Structural basis and molecular study
12. Substantial increase of protein stability by multiple disulphide bonds
13. 2.0 A structure of indole-3-glycerol phosphate synthase from the hyperthermophile Sulfolobus solfataricus: Possible determinants of protein stability
14. Insights into the molecular basis of thermal stability from the analysis of ion-pair networks in the glutamate dehydrogenase family
15. Tetrameric triosephosphate isomerase from hyperthermophilic Archaea
16. Effect of replacement of His-118, His-125 and Trp-143 by alanine on the catalytic activity and subunit assembly of inorganic pyrophosphatase from thermophilic bacterium PS-3
17. The crystal structure of triosephosphate isomerase (TIM) from Thermotoga maritima: A comparative thermostability structural analysis of ten different TIM structures
18. The crystal structure of Pyrococcus furiosus ornithine carbamoyltransferase reveals a key role for oligomerization in enzyme stability at extremely high temperatures
19. Isolation of the gene encoding Pyrococcus furiosus ornithine carbamoyltransferase and study of its expression profile in vivo and in vitro
20. Crystal structure of Pseudomonas aeruginosa catabolic ornithine transcarbamoylase at 3.0-A resolution: A different oligomeric organization in the transcarbamoylase family
21. Crystal structure at 2.8 A resolution of anabolic ornithine transcarbamylase from Escherichia coli
22. Substrate-induced conformational change in a trimeric ornithine transcarbamoylase
23. 1.85-A resolution crystal structure of human ornithine transcarbamoylase complexed with N-phosphonacetyl-L-ornithine. Catalytic mechanism and correlation with inherited deficiency
24. Arginine repression of the Saccharomyces cerevisiae ARG1 gene. Comparison of the ARG1 and ARG3 control regions
25. Regulation of arginine biosynthesis in Saccharomyces cerevisiae: Isolation of a cis-dominant, constitutive mutant for ornithine carbamoyltransferase synthesis
26. Multipurpose vectors designed for the fast generation of N- or C-terminal epitope-tagged proteins
27. Transformation of intact yeast cells treated with alkali cations
28. Catabolic ornithine carbamoyltransferase of Pseudomonas aeruginosa. Importance of the N-terminal region for dodecameric structure and homotropic carbamoylphosphate cooperativity
29. Determination of citrulline and allantoin and demonstration of citrulline in blood plasma
30. Modified methods for the determination of carbamyl aspartate
31. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
32. Protein thermostability above 100 degrees C: A key role for ionic interactions
33. Engineering activity and stability of Thermotoga maritima glutamate dehydrogenase. II: Construction of a 16-residue ion-pair network at the subunit interface