Increasing the efficiency of virus infectivity assays: Small inoculum volumes are as effective as centrifugal enhancement

Journal of Virological Methods

Volumn 98, Issue 2, 2001, Pages 167-169

  Reading, Steven A ^a   Edwards, Matthew J ^b   Dimmock, Nigel J ^a  

^a UNIVERSITY OF WARWICK   (United Kingdom)

^b UK   (United Kingdom)

Author keywords

Centrifugation; Enhancement; Infectivity; Influenza A virus

Indexed keywords

CELL RECEPTOR;

ARTICLE; CENTRIFUGATION; CONTROLLED STUDY; INFECTION; INFECTION RATE; INFLUENZA VIRUS A; INOCULATION; NONHUMAN; PRIORITY JOURNAL; VIROLOGY; VIRUS INFECTION; VIRUS PARTICLE;

ANIMALS; CELL LINE; CENTRIFUGATION; DOGS; INFLUENZA A VIRUS; KINETICS; ORTHOMYXOVIRIDAE INFECTIONS; RECEPTORS, VIRUS; TIME FACTORS; VIRION; VIRUS REPLICATION;

EID: 0034813924     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(01)00368-8     Document Type: Article

References (9)

1. Centrifugal enhancement of retroviral-mediated gene transfer
2. Retroviral infection is limited by Brownian motion
3. Two influenza A virus haemagglutinin-specific Fabs neutralize by inhibiting virus attachment to target cells, while neutralization by their IgGs is complex and occurs through fusion-inhibition and attachment-inhibition simultaneously
4. Centrifugal enhancement of human immunodeficiency virus type 1 infection and human cytomegalovirus gene expression in primary monocyte/macrophages in vitro
5. Murine cytomegalovirus binds reversibly to mouse embryo fibroblasts: Implications for quantitation and explanation of centrifugal enhancement
6. Cytomegalovirus infectivity: Analysis of the phenomenon of centrifugal enhancement of infectivity
7. Improved methods of retroviral vector transduction and production for gene therapy
8. Sensitivity and reproducibility in adenoviral infectious titer determination
9. Enhancement of infectivity of murine cytomegalovirus in vitro by centrifugal inoculation