Purification and characterization of two extracellular alkaline proteases from a newly isolated obligate alkalophilic bacillus sphaericus

Journal of Industrial Microbiology and Biotechnology

Volumn 26, Issue 6, 2001, Pages 387-393

  Singh, J ^a   Vohra, R M ^a   Sahoo, D K ^a  

^a INSTITUTE OF MICROBIAL TECHNOLOGY   (India)

Author keywords

Alkalophilic Bacillus; Properties; Serine protease; Substrate specificity

Indexed keywords

ALKALINE PROTEINASE; AMMONIUM SULFATE; ANAZOLENE SODIUM; CHYMOTRYPSIN; CHYMOTRYPSIN A; CHYMOTRYPSIN INHIBITOR; HEMOGLOBIN; IODOACETIC ACID; SERINE PROTEINASE; SERINE PROTEINASE INHIBITOR; SILVER; THIOL REAGENT; TOSYLLYSYL CHLOROMETHYL KETONE; TOSYLPHENYLALANYL CHLOROMETHYL KETONE; TRYPSIN INHIBITOR;

AMINO TERMINAL SEQUENCE; ARTICLE; BACILLUS SPHAERICUS; CELL FREE SYSTEM; CHROMATOGRAPHY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SPECIFICITY; HYDROPHOBICITY; MOLECULAR WEIGHT; NONHUMAN; PRECIPITATION; STAINING; ULTRAFILTRATION;

AMINO ACID SEQUENCE; BACILLUS; CHROMATOGRAPHY, ION EXCHANGE; ISOELECTRIC POINT; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; SERINE ENDOPEPTIDASES; SERINE PROTEINASE INHIBITORS; SUBSTRATE SPECIFICITY;

EID: 0034812939     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.jim.7000151     Document Type: Article

References (33)

1. Isolation and characterization of a subtilisin-like serine protease secreted by the sap-staining fungus Ophiostoma pieceae
2. Soap and detergents
3. Simplification of Heukeshoren and Derinc's silver staining of proteins
4. A sensitive new substrate for chymotrypsin
5. The preparation and properties of two new chromogenic substrates for trypsin
6. Purification and properties of an extracellular protease from Myxococcus virescens
7. Purification and characterization of an extracellular protease from alkaliphilic and thermophilic Bacillus sp. PS719
8. Structures of product and inhibitor complexes of Streptomyces griseus protease A at 1.8 Å resolution. A model for serine protease catalysis
9. Microbial proteinases
10. Molecular cloning of the structural gene for alkaline elastage YaB, a new subtilisin produced by alkalophilic Bacillus strain
11. Agric Biol Chem
12. Purification of alkaline protease from a Bacillus strain and their possible interrelationship
13. Cleavage of structural proteins during the assembly of head of bacteriophase T4
14. A new chromogenic substrate for subtilisin
15. Thermostable alkaline protease produced by Bacillus thermorubber - A new sp. of Bacillus
16. Sequence from picomoles quantities of proteins electroporated onto polyvinylidine difluoride membranes
17. Stability of two novel serine proteinases in commercial laundry detergent formulations
18. A rapid protein determination by modification of the Lowry procedure
19. Purification and properties of a novel surface-active agent and alkaline-resistant protease from Bacillus sp. Y
20. Alkaline protease from a new obligate alkalophilic isolate of Bacillus sphaericus
21. Purification and characterisation of two extracellular proteinases from Arthrobacter nicotinae 9458
22. Subtilisin Carlsberg V: The complete sequence; comparison with subtilisin BPN'; evolutionary relationship
23. Biochem Biophys Res Commun
24. Direct comparison of subtilisin-like intracellular pmtease of Bacillus licheniformis with the homologous enzymes of Bacillus subtilis
25. Characterisation of an alkaline protease from Bacillus sp. no. AH-101
26. Enzymes in powdered and liquid laundry detergents
27. Purification and properties of the chymotrypsin-like serine proteinase overproduced by Streptomyces sp. strain C5-A13
28. Subtilisin Sendal from alkalophilic Bacillus sp.: Molecular and enzymatic properties of the enzyme and molecular cloning and characterization of the gene, aprS
29. Purification and some properties of Bacillus sphaericus protease
30. Proteolytic components of alkaline proteases of Bacillus strains, zymograms and electrophoretic isolation