Lipase and its modulator from Pseudomonas sp. strain KFCC 10818: Proline-to-glutamine substitution at position 112 induces formation of enzymatically active lipase in the absence of the modulator

Journal of Bacteriology

Volumn 183, Issue 20, 2001, Pages 5937-5941

  Kim, E K ^a   Jang, W H ^a   Jung Ho Ko, ^a   Jong Seok Kang, ^a   Moon Jong Noh, ^a   Yoo, O J ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME VARIANT; GLUTAMINE; PROLINE; REGULATOR PROTEIN; TRIACYLGLYCEROL LIPASE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVITY; ENZYME SYNTHESIS; GENE EXPRESSION; GENE LOCATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FOLDING; PSEUDOMONAS; REGULATOR GENE; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY;

BACTERIAL PROTEINS; ENZYME ACTIVATION; GLUTAMINE; LIPASE; MOLECULAR CHAPERONES; MOLECULAR SEQUENCE DATA; MUTATION; PROLINE; PROTEIN FOLDING; PSEUDOMONAS; VARIATION (GENETICS);

ESCHERICHIA COLI; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS SP.;

EID: 0034811043     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.20.5937-5941.2001     Document Type: Article

References (35)

1. Chaperone-mediated activation in vivo of a Pseudomonas cepacia lipase
2. Identification of the tliDEF ABC transporter specific for lipase in Pseudomonas fluorescens SIK W1
3. Cloning, sequencing and expression of the lipase gene from Pseudomonas fragi IFO-12049 in E. coli
4. Bacterial lipolytic enzymes: Classification and properties
5. The expression in E. coli of a polymeric gene coding for an esterase mimic catalyzing the hydrolysis of p-nitrophenyl esters
6. Specificity, efficiency, and fidelity of PCR
7. Purification, molecular cloning, and expression of lipase from Pseudomonas aeruginosa
8. Cloning and nucleotide sequence of thermostable lipase gene from Pseudomonas fluorescens SIK W1
9. Specificity of the lipase-specific foldases of gram-negative bacteria and the role of the membrane anchor
10. Role of the lipase-specific foldase of Burkholderia glumae as a steric chaperone
11. Cloning of the Pseudomonas glumae lipase gene and determination of the active site residues
12. An accessory gene, lipB, required for the production of active Pseudomonas glumae lipase
13. Role of the lipB gene product in the folding of the secreted lipase of Pseudomonas glumae
14. Pseudomonas lipases: Biochemical properties and molecular cloning
15. Activation of a bacterial lipase by its chaperone
16. Requirement in trans of the downstream limL gene for activation of lactonizing lipase from Pseudomonas sp. 109
17. Lipase modulator protein (LimL) of Pseudomonas sp. strain 109
18. Bacterial lipases
19. Characterization of an alkaline serine protease from an alkaline-resistant Pseudomonas sp.: Cloning and expression of the protease gene in Escherichia coli
20. Cloning, sequence, and expression of a lipase gene from Pseudomonas cepacia: Lipase production in heterologous hosts requires two Pseudomonas genes
21. The ABC-exporter genes involved in the lipase secretion are clustered with the genes for lipase, alkaline protease, and serine protease homologues in Pseudomonas fluorescens no. 33
22. Cloning, sequencing and expression of the amylase isozyme from Pseudomonas sp. KFCC 10818
23. The crystal structure of a triacylglycerol lipase from Pseudomonas cepacia reveals a highly open conformation in the absence of a bound inhibitor
24. Enhancement of thermostability and catalytic efficiency of AprP, an alkaline protease from Pseudomonas sp., by the introduction of a disulfide bond
25. Specific and sensitive plate assay for bacterial lipases
26. Molecular cloning and nucleotide sequence of the lipase gene from Pseudomonas fragi
27. A procedure for the isolation of deoxyribonucleic acid from microorganisms
28. Crystal structure of Pseudomonas aeruginosa lipase in the open conformation
29. The crystal structure of triacylglycerol lipase from Pseudomonas glumae reveals a partially redundant catalytic aspartate
30. Lipase from Pseudomonas aeruginosa. Production in Escherichia coli and activation in vitro with a protein from the downstream gene
31. High-level formation of active Pseudomonas cepacia lipase after heterologous expression of the encoding gene and its modified chaperone in Escherichia coli and rapid in vitro refolding
32. DNA sequencing with chain-terminating inhibitors
33. Protein secretion in Pseudomonas aeruginosa
34. Molecular genetics of the extracellular lipase of Pseudomonas aeruginosa PAO1
35. In vitro analysis of roles of a disulfide bridge and a calcium binding site in activation of Pseudomonas sp. strain KWI-56 lipase