A new method for the isolation of histatins 1, 3, and 5 from parotid secretion using zinc precipitation

Protein Expression and Purification

Volumn 23, Issue 1, 2001, Pages 198-206

  Flora, Bianca ^a   Gusman, Heloisa ^a   Helmerhorst, Eva J ^a   Troxler, Robert F ^a,b   Oppenheim, Frank G ^a,b  

^a BOSTON UNIVERSITY   (United States)

^b BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTICAL PROCEDURES AND TECHNIQUES; HISTATIN; PRECIPITATION; PROTEIN ISOLATION; PROTEIN PURIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SALIVA PROTEIN; SOLUBILITY; ZINC;

EID: 0034805369     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1493     Document Type: Article

References (36)

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3. Structural relationship between human salivary histatins
4. Anticandidal activity of major human salivary histatins
5. Synthetic histatin analogues with broad-spectrum antimicrobial activity
6. Candida albicans mutants deficient in respiration are resistant to the small cationic salivary antimicrobial peptide histatin 5
7. Growth-inhibitory and bactericidal effects of human parotid salivary histidine-rich polypeptides on Streptococcus mutans
8. Salivary histatin 5 is an inhibitor of both host and bacterial enzymes implicated in periodontal disease
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10. Is salivary histatin 5 a metallopeptide?
11. Zinc and copper bind to unique sites of histatin 5
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21. The formation of protein precipitates and their centrifugal recovery
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24. Polyacrylamide gel electrophoresis of human salivary histidine-rich-polypeptides
25. The primary structure and functional characterization of the neutral histidine-rich polypeptide from human parotid secretion
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27. Mass spectrometric analysis of proteins
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33. Complete covalent structure of statherin, a tyrosine-rich acidic peptide which inhibits calcium phosphate precipitation from human parotid saliva
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