Purification of brain peroxisomes and localization of 3-hydroxy-3-methylglutaryl coenzyme A reductase

European Journal of Biochemistry

Volumn 268, Issue 18, 2001, Pages 4850-4859

  Kovacs, Werner J ^a   Faust, Phyllis L ^b   Keller, Gilbert Andre ^a,c   Krisans, Skaidrite K ^a  

^a SAN DIEGO STATE UNIVERSITY   (United States)

^b Och Spine at New York Presbyterian Hospitals   (United States)

^c GENENTECH INC   (United States)

Author keywords

Cholesterol; CNS; HMG CoA reductase; Myelin; Peroxisome

Indexed keywords

2',3' CYCLIC NUCLEOTIDE 3' PHOSPHODIESTERASE; ANTIBODY; CATALASE; CHOLESTEROL; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE; MARKER;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN CELL; BRAIN MITOCHONDRION; BRAIN STEM; CELL FRACTIONATION; CELL FREE SYSTEM; CELL ISOLATION; CELL ORGANELLE; CELLULAR PARAMETERS; CENTRAL NERVOUS SYSTEM; CEREBELLUM; CHOLESTEROL BLOOD LEVEL; CONTROLLED STUDY; DENSITY GRADIENT CENTRIFUGATION; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; ENZYME LOCALIZATION; FEMALE; IMMUNOBLOTTING; IMMUNOELECTRON MICROSCOPY; MALE; MOUSE; MYELIN SHEATH; NONHUMAN; PEROXISOME; POSTNATAL DEVELOPMENT; PRIORITY JOURNAL; PURIFICATION; SPINAL CORD; TISSUE LEVEL;

2',3'-CYCLIC-NUCLEOTIDE PHOSPHODIESTERASES; ANIMALS; BRAIN STEM; CATALASE; CENTRAL NERVOUS SYSTEM; CENTRIFUGATION, DENSITY GRADIENT; CEREBELLUM; CHOLESTEROL; ENDOPLASMIC RETICULUM; HYDROXYMETHYLGLUTARYL COA REDUCTASES; HYDROXYMETHYLGLUTARYL-COA-REDUCTASES, NADP-DEPENDENT; MICE; MICE, INBRED ICR; MICROSCOPY, IMMUNOELECTRON; PEROXISOMES; PHOSPHORIC DIESTER HYDROLASES; SPINAL CORD;

ANIMALIA;

EID: 0034797095     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02409.x     Document Type: Article

References (35)

1. Cell compartmentalization of cholesterol biosynthesis
2. Biogenesis of peroxisomes
3. Import of peroxisomal matrix and membrane proteins
4. Characterization of phosphomevalonate kinase
5. Cloning and subcellular localization of hamster and rat isopentenyl diphosphate dimethylally diphosphate isomerase. A PTS1 motif targets the enzymes to peroxisomes
6. Identification of peroxisomal targeting signals in cholesterol biosynthetic enzymes:acetoacetyl-CoA thiolase, HMG-CoA synthase, mevalonate diphosphate decarboxylase and farnesyl diphosphate synthase
7. Cholesterol for synthesis of myelin is made locally and not imported into brain
8. Origin of cholesterol
9. Cholesterol and myelin
10. The SREBP pathway: Regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor
11. Purification of peroxisomal fraction from rat brain
12. Purification of peroxisomes and subcellular distribution of enzyme activities for activation and oxidation of very-long-chain fatty acids in rat brain
13. Postnatal development and isolation of peroxisomes from brain
14. Mitochondrial and peroxisomal β-oxidation of stearic and lignoceric acids by rat brain
15. Characterization of peroxisomal 3-hydroxy-3-methylglutaryl coenzyme A reductase in UT2* cells
16. Mevalonate kinase is predominantly localized in peroxisomes and is defective in patients with peroxisome deficiency disorders
17. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding
18. Differential expression of 2′:3′-cyclic nucleotide 3′-phosphodiesterase in cultured central, peripheral, and extraneural cells
19. Characterization of UT2 cells
20. Phosphorylation of native 97-kDa 3-hydroxy-3-methylglutaryl-coenzyme A reductase from rat liver. Impact on activity and degradation of the enzyme
21. Acyl-CoA synthetase in rat liver peroxisomes
22. Microperoxisomes in the central nervous system of the postnatal rat
23. Cellular and subcellular distribution of 2′,3′-cyclic nucleotide 3′-phosphodiesterase and its mRNA in the rat central nervous system
24. Distribution of lipids in subcellular particles of guinea-pig brain
25. Protein determinants of myelination in different regions of developing rat central nervous system
26. Peroxisomes in nervous tissue
27. Presence of heterogeneous peroxisomal populations in the rat nervous tissue
28. Purification and properties of acyl-CoA oxidase from rat liver
29. Peroxisome subpopulations of the rat liver: Isolation by immune free flow electrophoresis
30. Biogenesis of peroxisomes: Isolation and characterization of two distinct peroxisomal populations from normal and regenerating rat liver
31. A possible relationship between concentration of microperoxisomes and myelination
32. Is there a relationship between HMG-CoA reductase activity and forebrain pathology in the PKU mouse?
33. Cholesterol deficit but not accumulation of aberrant sterols is the major cause of the teratogenic activity in the Smith-Lemli-Opitz syndrome animal model
34. Peroxisomal disorders: Genotype, phenotype, major neuropathologic lesions, and pathogenesis