Simple, directional cDNA cloning for in situ transcript hybridization screens

BioTechniques

Volumn 31, Issue 4, 2001, Pages 938-946

  Tamme, R ^a   Mills, K ^a   Rainbird, B ^a   Nornes, S ^a   Lardelli, M ^a  

^a UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; CLONING; DNA; GENES; TISSUE;

SITU TRANSCRIPT HYBRIDIZATION;

BIOTECHNOLOGY;

COMPLEMENTARY DNA; OLIGONUCLEOTIDE;

3' UNTRANSLATED REGION; ANIMAL TISSUE; ARTICLE; DNA SYNTHESIS; GENE EXPRESSION; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC TRANSCRIPTION; IN SITU HYBRIDIZATION; MAGNET; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; RNA PROBE; SEQUENCE ANALYSIS; ZEBRA FISH;

EID: 0034793049     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01314rr05     Document Type: Article

References (19)

1. A large-scale insertional mutagenesis screen in zebrafish
2. Cleavage of the BMP-4 antagonist chordin by zebrafish tolloid
3. A comprehensive set of sequence analysis programs for the VAX
4. Gene walking by unpredictably primed PCR
5. Gene expression screening in Xenopus identifies molecular pathways, predicts gene function and provides a global view of embryonic patterning
6. Molecular identification of spadetail: Regulation of zebrafish trunk and tail mesoderm formation by T-box genes
7. The identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio
8. Tissue in situ hybridisation
9. Construction of an equalized cDNA library from Arabidopsis thaliana
10. Generating bacteriophage λ sublibraries enriched for rare clones
11. Inverted terminal repeats permit the average length of amplified DNA fragments to be regulated during preparation of cDNA libraries by polymerase chain reaction
12. Targeted gene walking by low stringency polymerase chain reaction: Assignment of a putative human brain sodium channel gene (SCN3A) to chromosome 2q24-q31
13. Gene-expression screens in vertebrate embryos: More than meets the eye
14. Targeted gene walking polymerase chain reaction
15. A polymerase chain reaction mediated by a single primer: Cloning of genomic sequences adjacent to a serotonin receptor protein coding region
16. Nonspecific, nested suppression PCR method for isolation of unknown flanking DNA
17. Random primed gene walking PCR: A simple procedure to retrieve nucleotide fragments adjacent to known DNA sequences
18. Three novel Notch genes in zebrafish: Implications for vertebrate Notch gene evolution and function