Cloning and partial characterization of the putative rifamycin biosynthetic gene cluster from the actinomycete Amycolatopsis mediterranei DSM 46095

Microbiological Research

Volumn 156, Issue 3, 2001, Pages 239-246

  Kaur, Hardeep ^a   Cortes, Jesus ^b   Leadlay, Peter ^c   Lal, Rup ^a  

^a UNIVERSITY OF DELHI   (India)

^b GLAXOSMITHKLINE   (United Kingdom)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Mediterranei; Rif PKS; Rifamycin A

Indexed keywords

AMINO ACID SEQUENCE; DNA HYBRIDIZATION; DNA SEQUENCE; FUNGAL GENE; GENE CLUSTER; GENE PROBE; GENETIC DIFFERENCE; GENETIC SCREENING; GENETIC STRAIN; GENETIC VARIABILITY; MOLECULAR CLONING; OLIGONUCLEOTIDE; POLYMERASE CHAIN REACTION; RIFAMPICIN; SYNTHETIC DNA;

BACTERIUM;

AMYCOLATOPSIS MEDITERRANEI;

EID: 0034786650     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1078/0944-5013-00108     Document Type: Article

References (16)

1. Basic local alignment search tool
2. Biosynthesis of the ansamycin antibiotic rifamycin: Deductions from the molecular analysis of the rif biosynthetic gene cluster for Amycolatopsis mediterranei S699
3. 6-Deoxyerythronolide B-synthase 2 from Saccharopolyspora erythraea. Cloning of the structural gene, sequence analysis and inferred domain structure of the mutifunctional enzyme
4. The rifamycins: Properties, biosynthesis and fermentation
5. Selection of suitable marker genes for the development of cloning vectors and electroporation in different strains of Amycolatopsis mediterranei
6. Construction of a hybrid plamid capable of replication in Amycolatopsis mediterranei
7. Rifamycins strain improvement programme
8. Engineering antibiotic producers to overcome the limitations of strain improvement programme
9. Development of an improved cloning vector and transformation system in Amycolatopsis mediterranei (Nocardia mediterranei)
10. Regulation and manipulation of gene clusters encoding type-I PKSs
11. A Sorangium cellulosum (Myxobacterium) gene cluster for the biosynthesis of the macrolide antibiotic soraphen A: Cloning, characterization and homology to polyketide synthase genes from actinomycetes
12. Cloning and sequence analysis of the putative rifamycin polyketide synthase gene cluster from Amycolatopsis mediterranei
13. Characterization of the enzymatic domains in the modular polyketide synthase involved in rifamycin B biosynthesis by Amycolatopsis mediterranei
14. The importance of homologus recombination in the generation of large deletions in hybrid plasmids in A. mediterranei