Reaction of hen egg white lysozyme with Fischer-type metallocarbene complexes: Characterization of the conjugates and determination of the metal complex binding sites

European Journal of Biochemistry

Volumn 268, Issue 20, 2001, Pages 5479-5487

  Salmain, Michèle ^a   Blais, Jean Claude ^b   Tran Huy, Hoa ^c   Compain, Carine ^c   Jaouen, Gérard ^a  

^a PSL RESEARCH UNIVERSITY   (France)

^b SORBONNE UNIVERSITÉ   (France)

^c LABORATOIRE HÉTÉROÉLÉMENTS ET COORDINATION   (France)

Author keywords

Fischer type metallocarbene complexes; Lysine residues; Lysozyme; MALDI TOF MS

Indexed keywords

AMINO ACID; CARBENOID; EGG WHITE; LYSINE DERIVATIVE; LYSOZYME; METALLOPROTEIN; MONOAMINE; PROTEIN; TUNGSTEN;

AMINO ACID ANALYSIS; ANALYTIC METHOD; ARTICLE; ATOM; BINDING SITE; COMPLEX FORMATION; CONJUGATE; CONJUGATION; CRYSTAL STRUCTURE; ENZYME MECHANISM; HEN; LYSOSOME; MASS SPECTROMETRY; METAL BINDING; MOLECULAR STABILITY; MOLECULAR WEIGHT; MOLECULE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PROTEIN INTERACTION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

ANIMALS; BINDING SITES; CHICKENS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; EGG PROTEINS; FEMALE; HYDROCARBONS; METHANE; MURAMIDASE; ORGANOMETALLIC COMPOUNDS; PEPTIDE MAPPING; PROTEIN BINDING; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRYPSIN;

EID: 0034786429     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02489.x     Document Type: Article

References (26)

1. Screening for heavy-atom derivatives and obtaining accurate isomorphous differences
2. Labeling of proteins by a triosmium carbonyl cluster via Bolton-Hunter-like procedure
3. Use of heavy metal clusters in the design of N-succinimidyl ester acylation reagents for side chain specific labeling of proteins
4. Side-chain selective and covalent labelling of proteins by organometallic complexes of heavy transition metals. Possible application in radiocrystallography of proteins
5. Transition metal ('Fischer-type') carbene complexes as protein labelling reagents
6. Relative effectiveness of various ions on the solubility and crystal growth of lysozyme
7. Phenylmethoxycarben- und methylmethoxycarben-pentacarbonyl-chrom-molybdän-wolfram und -cyclopentadienyl-dicarbonyl-mangan
8. Detection of chitinase activity after polyacrylamide gel electrophoresis
9. Vertebrate lysozymes
10. A convenient preparation of glycol chitin, a substrate of lysozyme
11. Selective modification of aspartic acid-101 in lysozyme by carbodiimide method
12. Stoichiometric reactions of transition metal carbene complexes
13. Mass spectroscopic analysis of mercury incorporation into proteins for X-ray diffraction phase determination
14. Physical organic chemistry of transition metal carbene complexes. 2. Kinetics and mechanism of reactions of [methyloxy (phenyl) carbene]penta-carbonylchromium (0) with primary amines in aqueous aceto-nitrile
15. Physical organic chemistry of transition metal carbene complexes. 7. Kinetics of hydrolysis of (CO)5M=C(OR)Ph (M=Cr, W; R.=Me, Et) and (CO)5Cr=C(OMe)CH=CHPh in aqueous acetro-nitrile
16. Physical organic chemistry of transtion metal carbene complexes. 5. Kinetics and mechanism of hydrolysis of (CO) 5Cr=C (OCH3) CH3 and (CO) 5Cr=C (OCH2CH3) CH3 in aqueous acetonitrile
17. Preparations and immunological characterizations of two lysozyme derivatives dinitrophenylated at lysine-33 and lysine-96, respectively
18. Acetylation of lysozyme Part II. Mechanism of lysis by lysozyme
19. Structure of hen egg-white lysozyme
20. Lysozyme revisited: Crystallographic evidence for distortion of an N-acetylmuramic acid residue bound in site D
21. Dinitrophenylation as a probe for the determination of environments of amino groups in protein. Reactivities of individual amino groups in lysozyme
22. Protein surface topology-probing by selective chemical modification and mass spectrometric peptide mapping
23. Identification of fluorescein-5′-isothiocyanate modification sites in proteins by electrospray-ionization mass spectrometry
24. Characterization of lysozyme-estrone glucuronide conjugates. The effect of the coupling reagent on the substitution level and sites of acylation
25. Environment and exposure to solvent of protein atoms. Lysozyme and insulin