Analysis of a putative voltage-gated prokaryotic potassium channel

European Journal of Biochemistry

Volumn 268, Issue 20, 2001, Pages 5386-5396

  Ungar, D ^a,c   Barth, A ^b   Haase, W ^a   Kaunzinger, A ^b   Lewitzki, E ^a   Ruiz, T ^a   Reilander H ^a,d   Michel, H ^a  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

^b GOETHE UNIVERSITY   (Germany)

^c PRINCETON UNIVERSITY   (United States)

^d SANOFI AVENTIS DEUTSCHLAND GMBH   (Germany)

Author keywords

E. coli Kch; Membrane protein; Overexpression; Potassium channel; Purification

Indexed keywords

CARDIOLIPIN; LIPID; PHOSPHATIDYLGLYCEROL; POTASSIUM CHANNEL; POTASSIUM ION; VOLTAGE GATED CHANNEL FORMING PROTEIN;

ARTICLE; CELL MEMBRANE; CONTROLLED STUDY; ESCHERICHIA COLI; ION PERMEABILITY; MEMBRANE COMPONENT; MEMBRANE POTENTIAL; NONHUMAN; POTASSIUM TRANSPORT; PRIORITY JOURNAL; PROKARYOTE; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

CELL MEMBRANE; CIRCULAR DICHROISM; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; ION CHANNEL GATING; POTASSIUM; POTASSIUM CHANNELS; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SOLUBILITY; SPECTROSCOPY, FOURIER TRANSFORM INFRARED;

EID: 0034785962     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02477.x     Document Type: Article

References (43)

1. Determination of the subunit stoichiometry of a voltage-activated potassium channel
2. Towards the three-dimensional structure of voltage-gated potassium channels
3. Alteration of voltage-dependence of Shaker potassium channel by mutations in the S4 sequence
4. + conduction
5. An Escherichia coli homologue of eukaryotic potassium channel proteins
6. + channels
7. A prokaryotic potassium ion channel with two predicted transmembrane segments
8. + channels in Escherichia coli and in Xenopus oocytes
9. +-uptake systems
10. +-transport ATPase of Escherichia coli
11. Bacterial ion channels
12. Bacterial mechanosensitive channels: Integrating physiology, structure and function
13. Transport across the bacterial outer-membrane
14. + channel from Escherichia coli
15. Determinant of cistron specificity in bacterial ribosomes
16. Isolation of differentiated membrane domains from Escherichia coli and Salmonella typhimurium, including a fraction containing attachment sites between the inner and outer membranes and the murein skeleton of the cell envelope
17. A rapid method of total lipid extraction and purification
18. Assay of inorganic phosphate, total phosphate and phosphatases
19. Coomassie Brilliant Blue staining of lipids on thin-layer plates
20. A new rapid method for the assay of bacterial fatty acids using high resolution capillary gas chromatography and trimethylsulfonium hydroxide
21. Peptide and protein molecular weight determination by electrophoresis using a high-molarity Tris buffer system without urea
22. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
23. Reconstitution of membrane proteins into liposomes: Application to energy-transducing membrane proteins
24. Bio-beads: An efficient strategy for two-dimensional crystallization of membrane proteins
25. Estimation of globular protein secondary structure from circular dichroism
26. Quantitative studies of the structure of proteins in solution by Fourier-transform infrared spectroscopy
27. Physicochemical methods in the study of biomembranes
28. Fine-structure enhancement: Assessment of a simple method to resolve overlapping bands in spectra
29. Over-production of proteins in E. coli: Mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels
30. Active increase in cardiolipin synthesis in the stationary growth phase and its physiological significance in Escherichia coli
31. Cyclopropane fatty acid synthase of Escherichia coli: deduced amino acid sequence, purification, and studies of the enzyme active site
32. Effect of temperature on the composition of fattty acids in Escherichia coli
33. Recombinant expression, purification and characterization of Kch, a putative Escherichia coli potassium channel protein
34. + channel (SKC1): Oligomeric stoichiometry and stability
35. + channel
36. Differentiation between transmembrane helices and peripheral helices by the deconvolution of circular dichroism spectra of membrane proteins
37. Identity of the Escherichia coli cls and nov genes
38. + current diversity is produced by an extended gene family conserved in Drosophila and mouse
39. + channels conserved in flies, mice and humans
40. + channel
41. The use and misuse of FTIR spectroscopy in the determination of protein structure
42. FTIR-Spectroscopy of multistranded coiled coil proteins
43. Model calculations on the amide-I infrared bands of globular proteins