Cloning, expression and characterization of a gene which encodes a topoisomerase I with positive supercoiling activity in pea

Plant Molecular Biology

Volumn 37, Issue 5, 1998, Pages 773-784

  Reddy, M K ^a   Nair, Suresh ^a   Tewari, Krishna K ^b  

^a INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY ICGEB   (India)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Heterologous expression; Introduction of positive supercoils in DNA; Pisum sativum; RACE PCR; Relaxation of DNA supercoils

Indexed keywords

AMINO ACID SEQUENCE; CDNA LIBRARY; CELL NUCLEUS; COMPLEMENTARY DNA; DNA FRAGMENT; DNA ISOLATION; DNA PROBE; DNA TOPOISOMERASE; ENZYME ACTIVITY; ENZYME LOCALIZATION; ENZYME PURIFICATION; GENE EXPRESSION; MAGNESIUM; MOLECULAR CLONING; MOLECULAR WEIGHT; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIMER DNA; WESTERN BLOTTING;

AMINO ACID SEQUENCE; CATIONS, DIVALENT; CELL NUCLEUS; CLONING, MOLECULAR; DNA TOPOISOMERASES, TYPE I; DNA, COMPLEMENTARY; DNA, PLANT; DNA, SUPERHELICAL; GENE EXPRESSION; GENES, PLANT; MAGNESIUM; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PEAS; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, DNA;

ARCHAEA; EMBRYOPHYTA; ESCHERICHIA COLI; EUKARYOTA; PISUM SATIVUM; SATIVUM;

EID: 0032125970     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006086311875     Document Type: Article

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