Structural comparison of recombinant human macrophage colony stimulating factor β and a partially reduced derivative using hydrogen deuterium exchange and electrospray ionization mass spectrometry

Protein Science

Volumn 10, Issue 11, 2001, Pages 2336-2345

  Zhang, Y Heidi ^a,b   Yan, Xuguang ^a,b   Maier, Claudia S ^a,b   Schimerlik, Michael I ^a   Deinzer, Max L ^a,b  

^a OREGON STATE UNIVERSITY   (United States)

^b UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Disulfide bond; Electrospray ionization mass spectrometry; Hydrogen deuterium exchange; Macrophage colony stimulating factor

Indexed keywords

DEUTERIUM; RECOMBINANT COLONY STIMULATING FACTOR; RECOMBINANT COLONY STIMULATING FACTOR BETA; UNCLASSIFIED DRUG;

ARTICLE; COMPARATIVE STUDY; CRYSTAL STRUCTURE; DISULFIDE BOND; HUMAN; ION EXCHANGE; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN STRUCTURE; PROTEIN TERTIARY STRUCTURE; STRUCTURE ANALYSIS; X RAY ANALYSIS;

AMINO ACID SEQUENCE; CELL LINE; CIRCULAR DICHROISM; DEUTERIUM; DISULFIDES; HUMANS; MACROPHAGE COLONY-STIMULATING FACTOR; MASS SPECTROMETRY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; OXIDATION-REDUCTION; PROTEIN FOLDING; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE;

EID: 0034769693     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.16701     Document Type: Article

References (12)

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4. Local perturbations by ligand binding of hydrogen deuterium exchange kinetics in a four-helix bundle protein, acyl coenzyme A binding protein (ACBP)
5. Investigating the glutatione-dependent in vitro folding pathway of homodimeric cytokine rhm-CSFb
6. Differentiation of the IL-3-dependent NFS-60 cell line and adaptation to growth in macrophage colony stimulating factor
7. Three-dimensional structure of dimeric human recombinant macrophage colony stimulating factor
8. Deuterium exchange mass spectrometry as a probe of protein kinase activation. Analysis of wild type and constitutively active mutants of MAP kinase kinase-1
9. Biology and action of colony-stimulating factor-1
10. Hydrogen exchange/electrospray ionization mass spectrometry studies of substrate and inhibitor binding and conformational changes of E. coli dihydrogenase reductase
11. A novel methodology for assignment of disulfide bond pairings in proteins
12. Determination of amide hydrogen exchange by mass spectrometry: A new tool for protein structure elucidation