A weak calcium binding site in subtilisin BPN′ has a dramatic effect on protein stability

Journal of the American Chemical Society

Volumn 118, Issue 7, 1996, Pages 1645-1650

  Kidd, Richard D ^a   Yennawar, Hemant P ^a   Sears, Pamela ^b   Wong, Chi Huey ^b   Farber, Gregory K ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; SUBTILISIN;

ARTICLE; ENZYME STRUCTURE; MUTATION; THERMOSTABILITY;

EID: 0029897902     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja953443p     Document Type: Article

References (47)

1. Wong, C.-H. Science 1989, 244, 1145-1152.
2. Abrahmsén, L..; Tom, J ; Burnier, J.; Butcher, K. A.; Kossiakoff, A.; Wells, J. A. Biochemistry 1991, 30, 4151-4159.
3. Wells, J A , Estell, D. A TIBS 1988, 13, 291-297.
4. Homandbera, G A.; Mattis, J. A., Laskowski, M., Jr. Biochemistry 1978, 17, 5220-5227
5. Zhong, Z.; Liu, J L.-C., Dinterman, L M.; Finkelman, M. A. J.; Mueller, W. T., Rollence, M L., Whitlow, M.; Wong, C.-H. J. Am Chem Soc. 1991, 113, 683-684.
6. Panotoliano, M W.; Whitlow, M.; Wood, J F , Dodd. S W.; Hardman, K. D., Rollence, M. L.; Bryan, P. N. Biochemistry 1989, 28, 7205-7213.
7. Wong, C -H.; Chen, S.-T., Hennen, W. J., Bibbs, J. A., Wang, Y.-F.; Liu, J L.-C.; Pantoliano, M. W : Whitlow, M ; Bryan, P. N. J. Am Chem. Soc. 1990, 112, 945-953
8. Sears, P ; Schuster, M.; Wang, P.; Witte, K., Wong, C.-H. J. Am. Chem Soc 1994, 116, 6521-6530
9. Pantoliano, M. W ; Whitlow, M.; Wood. J F.; Rollence, M. L.; Finzel, B. C.: Gilliland, G. L.; Poulos, T L.; Bryan, P. N. Biochemistry 1988, 27, 8311-8317
10. Kossiakoff, A. A ; Ultsch, M.; White, S., Eigenbrot, C Biochemistry 1991, 30, 1211-1221
11. Drenth, J., Hol, W. G. J ; Jansonius, J. N.; Koekoek, R. Eur. J. Biochem. 1972, 26, 177-181.
12. Bryan, P. N Biotechnol. Adv 1987, 5, 221-234
13. Yennawar, N. H ; Yennawar, H. P., Farber, G K. Biochemistry 1994, 33, 7326-7336.
14. Yennawar, H P., Yennawar, N. H., Farber, G. K J Am Chem. Soc 1995, 117, 577-585
15. Fitzpatrick, P A., Steinmetz, A. C. U.; Ringe, D , Klibanov, A. M. Proc. Natl Acad. Sci. U S A 1993, 90, 8653-8657
16. Carter, P ; Wells, J. Science 1987, 237, 394-399.
17. Jancarik, J.: Kim, S -H J. Appl. Crystallogr. 1991, 24, 409-411
18. Carter, C. W., Jr ; Carter, C. W. J Biol. Chem 1979, 254, 12219-12223
19. Monahan, J. E.; Schiffer, M.; Schiffer, J. P. Acta Crystallogr. 1967, 22, 322
20. Fletterick, R. J.; Sygusch, J., Murray, N.; Madsen, N. B.; Johnson, L. N. J Mol. Biol. 1976, 103, 1-13.
21. Brunger, A. T.; Kuriyan, J.; Karplus, M. Science 1987, 235, 458-460.
22. Brunger, A. X-PLOR Version 3.1: Yale University Press: New Haven, 1992, pp 75-77.
23. Jones, T. A.; Zou, J. Y.; Cowan, S. W.; Kjeldgaard, M. Acta Crystallogar. 1991, A47, 110-119.
24. Ploegman, J. H., Drent, G.; Kalk, K. H.; Hol, W. G. J. J. Mol. Biol. 1979, 127, 149-162.
25. Jocelyn, P C. Biochemistry of the SH Group: Academic Press: New York, 1972; pp 14-46.
26. Purdie, J. W J. Am. Chem. Soc. 1967, 89, 226-230.
27. Allison, W S. Acc. Chem Res. 1976, 9, 293-299
28. Syed, R.; Wu, Z.-P ; Hogle, J. M., Hilvert, D. Biochemistry 1993, 32, 6157-6164
29. Allen, F. H., Kennard, O.; Watson, D G., Brammer, L.; Orpen, A. G ; Taylor, R. J Chem. Soc., Perkin Trans. 2 1987, S1-S19.
30. Bode, W.; Papamokos, E., Musil, D. Eur. J. Biochem 1987, 166, 673-692.
31. Bott, R., Ultsch, M.; Kossiakoff, A., Graycar, T., Katz, B.; Power, S. J. Biol. Chem 1988, 263, 7895-7906.
32. Erwin, C. R., Barnett, B. L., Oliver, J D , Sullivan, J. F Protein Eng. 1990, 4, 87-97.
33. Gallagher, T ; Bryan, P.; Gilliland, G. L. Proteins. Str. Funct. Gen. 1993, 16, 205-213.
34. Ramaknshnan, C.; Ramachandran, G N Biochem. J. 1965, 5, 909-933.
35. Laskowski, R. A , MacArthur, M. W.; Moss, D. S.; Thornton, J M. J. Appl. Crystallogr. 1993, 26, 283-291.
36. Pantoliano, M. W.; Ladner, R. C.; Bryan, P. N.; Rollence, M. L.; Wood, J. F., Poulos. T. L. Biochemistry 1987, 26, 2077-2082.
37. Claiborne, A.; Miller, H ; Parsonage, D.; Ross, R. P. FASEB J. 1993, 7, 1483-1490.
38. Claiborne, A.; Ross, R. P.; Parsonage, D. Trends Biochem. Sci 1992, 17, 183-186.
39. Stehle, T.; Ahmed, S. A., Claiborne, A.; Schulz, G. E. J. Mol Biol. 1991, 221, 1325-1344.
40. Matsubara, H.; Hagihara, B.; Nakai, M ; Komaki, T ; Yonetani, T., Okunuki, K. J. Biochemistry 1958, 45, 251-258.
41. Braxton, S., Wells, J. A. Biochemistry 1992, 31, 7796-7801
42. Gros, P.; Kalk, K H.; Hol, W G. J. J Biol. Chem. 1991, 266, 2953-2961.
43. Toma, S.; Campagnoli, S., Margant. I , Gianna, R.; Grandi, G., Bolognesi, M.; DeFilippis, V., Fontana, A Biochemistry 1991, 30, 97-106
44. Strausberg, S. L., Alexander, P. A., Gallagher, D. T., Gilliland, G. L , Barnett B. L., Bryan, P N Bio/Technology 1995, 13, 669-673.
45. Heringa, J.; Argos, P ; Egmond, M. R.; de Vlieg, J. Prot. Eng. 1995, 8, 21-30
46. Hennga, J.; Argos, P. J. Mol. Biol. 1991, 220, 151-171.
47. Martinez, P.; Arnoldm F. J. Am. Chem. Soc. 1991, 113, 6336-6337.