A novel 20-kilodalton protein conserved in Babesia bovis and B. bigemina stimulates memory CD4+ T lymphocyte responses in B. bovis-immune cattle

Molecular and Biochemical Parasitology

Volumn 118, Issue 1, 2001, Pages 97-109

  Brown, Wendy C ^a   Ruef, Barbara J ^b   Norimine, Junzo ^a   Kegerreis, Kimberly A ^a   Suarez, Carlos E ^a   Conley, Patrick G ^b   Stich, Roger W ^a   Carson, Kenneth H ^b   Rice Ficht, Allison C ^b  

^a WASHINGTON STATE UNIVERSITY   (United States)

^b TEXAS A M COLLEGE OF MEDICINE   (United States)

Author keywords

Crystallin; Babesia bigemina; Babesia bovis; Bbg20; Bbo20; Small hsp

Indexed keywords

ALPHA CRYSTALLIN; EPITOPE; GAMMA INTERFERON; HEAT SHOCK PROTEIN; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2; PROTEIN; PROTEIN BBG20; PROTEIN BBO20; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; B LYMPHOCYTE; BABESIA BOVIS; CATTLE; CELL LINE; CELL PROLIFERATION; CELL STIMULATION; CONTROLLED STUDY; DNA LIBRARY; ELECTROPHORESIS; HAPLOTYPE; MEMORY; MEROZOITE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RABBIT; SOUTHERN BLOTTING; T LYMPHOCYTE;

AMINO ACID SEQUENCE; ANIMALS; ANTIGENS, PROTOZOAN; BABESIA; BABESIA BOVIS; BABESIOSIS; BLOTTING, SOUTHERN; CATTLE; CATTLE DISEASES; CD4-POSITIVE T-LYMPHOCYTES; CLONING, MOLECULAR; CONSERVED SEQUENCE; CRYSTALLINS; HEAT-SHOCK PROTEINS; IMMUNE SERA; IMMUNOLOGIC MEMORY; MOLECULAR SEQUENCE DATA; PROTOZOAN PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

BABESIA; BABESIA BIGEMINA; BABESIA BOVIS; BOS TAURUS; ORYCTOLAGUS CUNICULUS;

EID: 0034761106     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(01)00375-9     Document Type: Article

References (45)

1. Babesiosis
2. Human babesiosis: An emerging tick-borne disease
3. Immunopathophysiology of Babesia bovis and Plasmodium falciparum infections
4. Designing blood stage-vaccines against Babesia bovis and B. bigemina
5. Studies on the protection of cattle against Babesia bovis infection
6. The development of a recombinant Babesia vaccine
7. Mapping T cell epitopes of Babesia bovis antigen 12D3: Implications for vaccine design
8. Identification of two Th1 cell epitopes on the 77 kDa Babesia bovis merozoite protein (Bb-1) by use of truncated recombinant fusion proteins
9. Characterisation of a family of multi-copy genes encoding rhoptry protein homologues in Babesia bovis, Babesia ovis and Babesia canis
10. Babesia bovis: Dextran sulphate as an adjuvant for and precipitant of protective immunogens
11. Genetic and antigenic characterization of Babesia bovis merozoite spherical body protein Bb-1
12. Induction of proliferative responses of T Cells from Babesia bovis-immune cattle with a recombinant 77-kilodalton merozoite protein (Bb-1)
13. Babesia bovis: Gene isolation and characterization using a mung bean nuclease-derived expression library
14. Babesia bovis rhoptry-associated protein-1 is immunodominant for T helper cells of immune cattle and contains T cell epitopes conserved among geographically distant B. bovis strains
15. Characterization of the gene encoding a 60-kilodalton Babesia bovis merozoite protein with conserved and surface exposed epitopes
16. Use of Th cells to identify potential vaccine antigens of Babesia bovis
17. Immunity to asexual blood stages of Plasmodium: Is resistance to acute malaria adaptive or innate?
18. Reactive oxygen and nitrogen intermediates and products from polyamine degradation are babesiacidal in vitro
19. Babesia bovis-stimulated macrophages express interleukin-1β, interleukin-12, tumor necrosis factor-alpha, and nitric oxide, and inhibit parasite replication in vitro
20. Stimulation of nitric oxide production by Babesia bovis
21. + T cell clones activated in vitro
22. + T-lymphocyte clones specific for rhoptry associated protein-1 (RAP-1) of Babesia bigemina stimulate enhanced immunoglobulin G1 (IgG1) and IgG2 synthesis
23. Identification of Babesia bovis merozoite antigens separated by continuous-flow electrophoresis that stimulate proliferation of helper T cell clones derived from B. bovis-immune cattle
24. + T cell lines from genetically diverse cattle
25. The heat-shock proteins
26. Cloning and characterization of a bradyzoite-specifically expressed gene (hsp30/bag1) of Toxoplasma gondii, related to genes encoding small heat-shock proteins of plants
27. Cell-mediated immune responses to Babesia bovis antigens in cattle following infection with tick-derived or cultured parasites
28. Extensive polymorphism of the BoLA-DRB3 gene distinguished by PCR-RFLP
29. Babesia bigemina: Identification of B cell epitopes associated with parasitized erythrocytes
30. Helper T cell epitopes encoded by the Babesia bigemina rap-1 gene family in the constant and variant domains are conserved among parasite strains
31. A unique Babesia bovis spherical body protein is conserved among geographic isolates and localizes to the infected erythrocyte membrane
32. A simple method for displaying the hydropathic character of a protein
33. Identification of fetal liver tyrosine kinase 3 (Flt3) ligand domain required for receptor binding and function using naturally occurring ligand isoforms
34. A recombinant surface protein of Babesia bovis elicits bovine antibodies that react with live merozoites
35. Strain variation of Babesia bovis merozoite surface-exposed epitopes
36. + T-lymphocyte clones from protectively immunized cattle is diverse and includes major surface protein 2 (MSP-2) and MSP-3
37. A C-terminal signal prevents secretion of luminal ER proteins
38. Molecular characterization of Babesia bovis merozoite surface proteins bearing epitopes immunodominant in protected cattle
39. Type I and type II responses in cattle and their regulation
40. IFN-γ stimulates IgG2 production from bovine B cells costimulated with anti-μ and mitogen
41. Functional properties of bovine IgG1 and IgG2: Interaction with complement, macrophages, neutrophils, and skin
42. Development of babesial vaccines
43. Small heat shock proteins are molecular chaperones
44. Role of Toxoplasma gondii hsp70 and Toxoplasma gondii hsp30/bag1 in antibody formation and prophylactic immunity in mice experimentally infected with Toxoplasma gondii
45. Phylogenetic analysis with newly characterized Babesia bovis hsp70 and hsp90 provides strong support for paraphyly with the piroplasms