Obtusifoliol 14α-demethylase (CYP51) antisense Arabidopsis shows slow growth and long life

Biochemical and Biophysical Research Communications

Volumn 285, Issue 1, 2001, Pages 98-104

  Kushiro, Masayo ^a   Nakano, Takeshi ^b   Sato, Ken ^b   Yamagishi, Kazutoshi ^b   Asami, Tadao ^b   Nakano, Akihiko ^b   Takatsuto, Suguru ^c   Fujioka, Shozo ^b   Ebizuka, Yutaka ^a   Yoshida, Shigeo ^b  

^a UNIVERSITY OF TOKYO   (Japan)

^b RIKEN   (Japan)

^c JOETSU UNIVERSITY OF EDUCATION   (Japan)

Author keywords

Arabidopsis; Brassionosteroid; CYP51; Obtusifoliol 14 demethylase; Sterol; Sterol 14 demethylase; Transgenic

Indexed keywords

COMPLEMENTARY DNA; CYTOCHROME P450; OBTUSIFOLIOL; PHYTOSTEROL; STEROL 14ALPHA DEMETHYLASE; UNCLASSIFIED DRUG; OXIDOREDUCTASE; PRIMER DNA; RECOMBINANT PROTEIN; TRIAZOLE CYTOCHROME P 450 OBTUSIFOLIOL 14 DEMETHYLASE; TRIAZOLE CYTOCHROME P-450-OBTUSIFOLIOL-14-DEMETHYLASE;

ARABIDOPSIS; ARTICLE; CONTROLLED STUDY; DNA LIBRARY; EXPRESSED SEQUENCE TAG; GENE MUTATION; HORMONE SYNTHESIS; LIFESPAN; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PLANT GROWTH; PRIORITY JOURNAL; YEAST; AMINO ACID SEQUENCE; ENZYMOLOGY; GENETICS; GROWTH, DEVELOPMENT AND AGING; MOLECULAR CLONING; MOLECULAR GENETICS; TRANSGENIC PLANT;

ARABIDOPSIS;

AMINO ACID SEQUENCE; ARABIDOPSIS; BASE SEQUENCE; CLONING, MOLECULAR; CYTOCHROME P-450 ENZYME SYSTEM; DNA PRIMERS; EXPRESSED SEQUENCE TAGS; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS;

EID: 0034741032     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.5122     Document Type: Article

References (25)

1. A role for brassinosteroids in light-dependent development of Arabidopsis
2. Molecular genetic studies confirm the role of brassinosteroids in plant growth and development
3. The Arabidopsis dwf7/ste1 mutant is defective in the Δ7 sterol C-5 desaturation step leading to brassinosteroid biosynthesis
4. FACKEL is a sterol C-14 reductase required for organized cell division and expansion in Arabidopsis embryogenesis
5. A critical role of sterols in embryonic patterning and meristem programming revealed by the fackel mutants of Arabidopsis thaliana
6. Brassinosteroids
7. Structural and evolutionary studies on sterol 14α-demethylase P450 (CYP51), the most conserved P450 monooxygenase: II. Evolutionary analysis of protein and gene structures
8. 14DM) of Saccharomyces cerevisiae and rat liver for 24-methylene-24,25-dihydrolanosterol and 24,25-dihydrolanosterol
9. 14DM) of yeast: Difference between the substrate recognition by yeast and plant sterol 14α-demethylases
10. Properties and structural requirements for substrate specificity of cytochrome P-450-dependent obtusifoliol 14α-demethylase from maize (Zea mays) seedlings
11. Isolation and reconstitution of the heme-thiolate protein obtusifoliol 14α-demethylase from Sorghum bicolor (L.) Moench
12. Cloning and expression in Escherichia coli of the obtusifoliol 14α-demethylase of Sorghum bicolor (L.) Moench, a cytochrome P450 orthologous to the sterol 14α-demethylase (CYP51) from fungi and mammals
13. Cloning and functional expression in yeast of a cDNA coding for an obtusifoliol 14α-demethylase (CYP51) in wheat
14. Evidence for recycling of cytochrome P450 sterol 14-demethylase from the cis-golgi compartment to the endoplasmic reticulum (ER) upon saturation of the ER-retention mechanism
15. Overexpression of PRA2, a Rab/Ypt-family small GTPase from pea Pisum sativum, aggravates the growth defect of yeast ypt mutants
16. Mechanism of action of polymeric aurintricarboxylic acid, a potent inhibitor of protein-nucleoic acid interactions
17. L-DNA gene 5 controls the tissue-specific expression of chimeric genes carried by a novel type of Agrobacterium binary vector
18. Floral dip: A simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
19. Brassinosteroid-insensitive dwarf mutants of Arabidopsis accumulate brassinosteroids
20. Molecular diversity of sterol 14α-demethylase substrates in plants, fungi and humans
21. Optimized expression and catalytic properties of a wheat obtusifoliol 14α-demethylase (CYP51) expressed in yeast. Complementation of erg11Δ yeast mutants by plant CYP51
22. Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae. A review
23. In vitro selection of calli resistant to a triazole cytochrome-P-450-obtusifoliol-14-demethylase inhibitor from protoplasts of Nicotiana tabacum L. cv Xanthi