A role for brassinosteroids in light-dependent development of Arabidopsis

Science

Volumn 272, Issue 5260, 1996, Pages 398-401

  Li, Jianming ^a   Nagpal, Punita ^a,c   Vitart, Veronique ^a,d   McMorris, Trevor C ^b   Chory, Joanne ^a  

^a SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^d SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHYTOSTEROL; STEROID REDUCTASE;

ARABIDOPSIS; ARTICLE; DEVELOPMENTAL GENETICS; GENE MUTATION; LIGHT; NONHUMAN; PLANT GROWTH; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; STEROIDOGENESIS;

ARABIDOPSIS THALIANA;

EID: 0029873330     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5260.398     Document Type: Article

References (30)

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10. 2 det2 plants with recombination break points either in the m323-DET2 region (68 recombinants, two mapping populations) or in the DET2-nga168 interval (31 recombinants), and new CAPS markers were converted directly from YAC insert ends or derived from phage clones of an Arabidopsis genomic library isolated with YAC end probes (9).
11. 2 det2 plants with recombination break points either in the m323-DET2 region (68 recombinants, two mapping populations) or in the DET2-nga168 interval (31 recombinants), and new CAPS markers were converted directly from YAC insert ends or derived from phage clones of an Arabidopsis genomic library isolated with YAC end probes (9).
12. 2 det2 plants with recombination break points either in the m323-DET2 region (68 recombinants, two mapping populations) or in the DET2-nga168 interval (31 recombinants), and new CAPS markers were converted directly from YAC insert ends or derived from phage clones of an Arabidopsis genomic library isolated with YAC end probes (9).
13. 2 det2 plants with recombination break points either in the m323-DET2 region (68 recombinants, two mapping populations) or in the DET2-nga168 interval (31 recombinants), and new CAPS markers were converted directly from YAC insert ends or derived from phage clones of an Arabidopsis genomic library isolated with YAC end probes (9).
14. 2 det2 plants with recombination break points either in the m323-DET2 region (68 recombinants, two mapping populations) or in the DET2-nga168 interval (31 recombinants), and new CAPS markers were converted directly from YAC insert ends or derived from phage clones of an Arabidopsis genomic library isolated with YAC end probes (9).
15. 2 det2 plants with recombination break points either in the m323-DET2 region (68 recombinants, two mapping populations) or in the DET2-nga168 interval (31 recombinants), and new CAPS markers were converted directly from YAC insert ends or derived from phage clones of an Arabidopsis genomic library isolated with YAC end probes (9).
16. Cosmid and phage clones were isolated from two Arabidopsis genomic libraries [N Olszewski, F. Martin, F Ausubel, Nucleic Acids Res. 16, 10765 (1988); the lambda genomic library was provided by R. W. Davis (Stanford University)] by hybridization with yUP2C12, yUP6B10, YAC end probes, or cosmidderived probes Cosmid DNAs were transformed into det2-1 plants by a modified vacuum infiltration method [N. Bechtold, J Ellis, G. Pelletier, C. R. Acad Sci. Paris 316, 1194 (1993), A. F. Bent et al , Science 265, 1856 (1994)] to identify cosmids containing the DET2 gene
17. Cosmid and phage clones were isolated from two Arabidopsis genomic libraries [N Olszewski, F. Martin, F Ausubel, Nucleic Acids Res. 16, 10765 (1988); the lambda genomic library was provided by R. W. Davis (Stanford University)] by hybridization with yUP2C12, yUP6B10, YAC end probes, or cosmidderived probes Cosmid DNAs were transformed into det2-1 plants by a modified vacuum infiltration method [N. Bechtold, J Ellis, G. Pelletier, C. R. Acad Sci. Paris 316, 1194 (1993), A. F. Bent et al , Science 265, 1856 (1994)] to identify cosmids containing the DET2 gene
18. Cosmid and phage clones were isolated from two Arabidopsis genomic libraries [N Olszewski, F. Martin, F Ausubel, Nucleic Acids Res. 16, 10765 (1988); the lambda genomic library was provided by R. W. Davis (Stanford University)] by hybridization with yUP2C12, yUP6B10, YAC end probes, or cosmidderived probes Cosmid DNAs were transformed into det2-1 plants by a modified vacuum infiltration method [N. Bechtold, J Ellis, G. Pelletier, C. R. Acad Sci. Paris 316, 1194 (1993), A. F. Bent et al , Science 265, 1856 (1994)] to identify cosmids containing the DET2 gene
19. 6 clones of an Arabidopsis complementary DNA (cDNA) library constructed in lambda ZAPII [J. J. Kieber, M. Rothenburg, G Roman, K A Feldmann, J R. Ecker, Cell 72, 427 (1993)]. Positive clones were converted to plasmids by in vivo excision according to the manufacturer's protocol (Stratagene) and sequenced with gene-specific primers
20. The transcribed region of the DET2 gene was amplified by polymerase chain reaction (PCR) from genomic DNAs of wild-type Col-0 and eight det2 alleles, subcloned into pGEM-T vector (Promega), and sequenced. To minimize PCR errors, we pooled at least four different clones from two independently amplified fragments for sequencing
21. Database searches were performed at the U S National Center for Biotechnology Information with the BLAST program [S. F. Altschul, W Gish, W. Willer, E. W. Myers, D. J. Lipman, J Mol. Biol. 215, 403 (1990)]. The sequence alignment and phylogenetic analysis were performed with the Megalign program (DNAStar) by the method of J. Hein [D G Higgins and P. M Sharp, Comput Appl. Biosci 5, 151 (1989)].
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28. -5 M). Hormones were sterile-filtered into the cooling MS medium For dark-grown seedlings, seeds were exposed to 2-hour light treatment before their plates were wrapped with three layers of aluminum foil, and the seedlings were transferred under a green safe-light. The hypocotyl lengths of 10-day-old etiolated seedlings and 12-day-old lightgrown wild-type plants were measured.
29. Single-letter abbreviation for the amino acid residues are as follows: A, Ala; C, Cys, D, Asp; E, Glu, F, Phe; G, Gly; H, His: 1, Ile; K, Lys; L, Leu; M, Met; N, Asn, P, Pro; Q, Gln; R, Arg; S, Ser, T, The; V, Val; W, Trp; and Y, Tyr.
30. We thank K Hanson for technical assistance; S Clouse (North Carolina State University) for helpful discussions; and D. Weigel, P. Doemer, S. Worland, and members of our lab for critical reading of the manuscript. Supported by grants to J.C. from USDA (93-373019125) and the National Science Foundation (DIR-9116923).