Primary neural precursor cell expansion, differentiation and cytosolic Ca2+ response in three-dimensional collagen gel

Journal of Neuroscience Methods

Volumn 102, Issue 2, 2000, Pages 187-195

  O'Connor, Stephen M ^a   Stenger, David A ^a   Shaffer, Kara M ^b   Maric, Dragan ^c   Barker, Jeffery L ^c   Ma, Wu ^a  

^a NAVAL RESEARCH LABORATORY   (United States)

^b SCIENCE APPLICATIONS INTERNATIONAL CORPORATION   (United States)

^c NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE   (United States)

Author keywords

Ca2+ imaging; Collagen; Differentiation; Immunocytochemistry; Neural precursor cell; Proliferation; Rat cerebral cortex; Three dimensional matrix

Indexed keywords

COLLAGEN; COLLAGEN GEL;

ANIMAL CELL; ARTICLE; BRAIN CORTEX; CALCIUM TRANSPORT; CELL DIFFERENTIATION; CELL PROLIFERATION; CONTROLLED STUDY; IMMOBILIZED CELL; IMMUNOCYTOCHEMISTRY; NERVOUS SYSTEM DEVELOPMENT; NEUROEPITHELIUM; NONHUMAN; PRECURSOR CELL; PRIORITY JOURNAL; RAT; STEM CELL;

ANIMAL; ASTROCYTES; CALCIUM; CELL DIFFERENTIATION; CELL DIVISION; CELL SURVIVAL; CELLS, CULTURED; CEREBRAL CORTEX; COLLAGEN; CULTURE MEDIA, SERUM-FREE; CYTOSOL; FIBROBLAST GROWTH FACTOR 2; GELS; GLUTAMIC ACID; NEURONS; OSCILLOMETRY; RATS; RATS, SPRAGUE-DAWLEY; STEM CELLS; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S.;

EID: 0034735717     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(00)00303-4     Document Type: Article

References (45)

1. Fetal neuron grafts pave the way for stem cell therapies
2. Review: tissue engineering in the nervous system
3. Reconstruction of peritoneal-like structure in three-dimensional collagen gel matrix culture
4. Influence of fetal calf serum, fibroblast growth factors, and hepatocyte growth factor on three-dimensional cultures of human keratocytes in collagen gel matrix
5. Regeneration and proliferation of embryonic and adult rat hippocampal neurons in culture
6. Optimized survival of hippocampal neurons in B27 supplemented Neurobasal. A new serum-free medium combination
7. Perspectives on cell and collagen composites for tendon repair
8. Feasibility of electrical recordings from unconnected vertebrate CNS neurons cultured in a three-dimensional extracellular matrix
9. Collagen Matrix: an attractive alternative to agar and methylcellulose for the culture of hematopoietic progenitors in autologous transplantation products
10. Neural precursor cells: applications for the study and repair of the central nervous system
11. Collagen-biomaterial for drug delivery
12. Cell therapy
13. Stem cells of the central nervous system
14. Mammalian neural stem cells
15. Prospects for the clinical application of neural transplantation with the use of conditionally immortalized neuroepithelial stem cells
16. Cultured human nasal gland cells in a three-dimensional collagen gel
17. Three-dimensional cell aggregation enhance growth-promoting activity of NGF in adult DRG
18. Three-dimensional cultures of fetal mouse cerebral cortex in a collagen matrix
19. Primary culture of marrow core in collagen gels: Modulation and transformation of endosteal cells. I. Morphologic Observations
20. Cell aggregation and neurite outgrowth in gels of extracellular matrix molecules
21. Collagen gels as a matrix for haemopoiesis
22. The expression and posttranslational modification of a neuron-specific β-tubulin isotope during chick embryogenesis
23. Biologic biomaterials: tissue-derived biomaterials (collagen)
24. Basic FGF-responsive telencephalic precursor cells express functional GABA(A) receptor/Cl- channels in vitro
25. Acetylcholine stimulates cortical precursor cell proliferation via muscarinic receptor activation and MAP kinase phosphorylation
26. Cultures of cells from fetal rat brain: methods to control composition, morphology, and biochemical activity
27. The business of stem cells
28. Stem cells in the CNS
29. Expression of neuron-specific tubulin defines a novel population in the proliferative layers of the developing telencephalon
30. In vitro rapid organization of endothelial cells into capillary-like networks is promoted by collagen matrices
31. Immobilization of neural cells in three-dimensional matrices for biosensor applications
32. Development and application of cell-based biosensors
33. Neural stem and progenitor cells: a strategy for gene therapy and brain repair
34. Regulation of voltage- and ligand-gated currents in rat hippocampal progenitor cells in vitro
35. Role of extracellular matrix in neural development
36. Neural stem-like cells: developmental lessons with therapeutic potential
37. Unilocular fat cells in three-dimensional collagen gel matrix culture
38. A method for the isolation and culture of human colonic crypts in collagen gels
39. Synthetic polymer derivatives as substrata for neuronal adhesion and growth
40. Cultured rat neuronal and glial cells entrapped within hydrogel polymer matrices: a potential tool for neural tissue replacement
41. Porous hydrogels for neural tissue engineering
42. Tissue regeneration templates based on collagen-glycosaminoglycan copolymers
43. Use of mesenchymal stem cells in a collagen matrix for achilles tendon repair