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2ATP (ATP, adenosine 5′-triphosphate); pH 7.2. Resistances of the pipettes were 5 to 10 megohms. The indicated holding potentials were corrected for liquid junction potentials (-4 mV) for the I-V relations only (Fig. 3C). Voltages were not corrected for the voltage drop across the uncompensated series resistance. Experiments were done at room temperature (20° to 25°C). Data were filtered at 2 kHz and digitized at 5 or 20 kHz with PClamp6 software.
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10
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note
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Extracellular solutions with elevated potassium or diluted drugs were applied with a glass pipette (150-μm tip diameter) positioned at ∼300 μm from the recorded IHC. Application of elevated potassium changed membrane potentials with time constants of ∼1 s.
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note
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We thank A. Rusch for helpful comments on the manuscript. This work was supported by grant DC 00276 from the National Institute on Deafness and Other Communication Disorders.
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