Identification of the subunits of the nicotinic cholinergic receptors in the rat cochlea using RT-PCR and in situ hybridization

Molecular Brain Research

Volumn 53, Issue 1-2, 1998, Pages 78-87

  Morley, Barbara J ^a   Li, Ha Sheng ^b   Hiel, Hakim ^a   Drescher, Dennis G ^c   Elgoyhen, Ana B ^d  

^a BOYS TOWN NATIONAL RESEARCH HOSPITAL   (United States)

^b NATIONAL INSTITUTE ON DEAFNESS AND OTHER COMMUNICATION DISORDERS   (United States)

^c Laboratory of Bio otology   (United States)

^d Instituto de Investigaciones Farmacologicas   (Argentina)

Author keywords

Cochlea; Gene expression; Hair cells; In situ hybridization; mRNA; Nicotinic acetylcholine receptor subunits; RT PCR; Spiral ganglion

Indexed keywords

MESSENGER RNA; NICOTINIC RECEPTOR; RECEPTOR SUBUNIT; SULFUR 35;

ANIMAL TISSUE; ARTICLE; COCHLEA; CORTI ORGAN; FEMALE; GENE EXPRESSION; HAIR CELL; IN SITU HYBRIDIZATION; ISOTOPE LABELING; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA PROBE; SPIRAL GANGLION;

ANIMALS; COCHLEA; DENDRITES; DNA PRIMERS; FEMALE; HAIR CELLS, OUTER; IN SITU HYBRIDIZATION; MACROMOLECULAR SUBSTANCES; MALE; MICE; NERVE FIBERS; NEURONS, EFFERENT; ORGAN OF CORTI; POLYMERASE CHAIN REACTION; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, NICOTINIC; SPIRAL GANGLION;

ANIMALIA; MAMMALIA;

EID: 0031883931     PISSN: 0169328X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0169-328X(97)00272-6     Document Type: Article

References (44)

1. R.A. Altschuler, J. Fex, Efferent neurotransmitters. In: R.A. Altschuler, R.P. Bobbin, D.W. Hoffman (Eds.), Neurobiology of Hearing: The Cochlea, Raven, New York, 1986, pp. 383-396.
2. Anand R., Fuchs P.A., Cooper J.F., Peng X., Lindstrom J. Evidence that α-bungarotoxin-sensitive acetylcholine receptors in chick's cochlea contain α7 subunits. Soc. Neurosci. Abstr. 19:1993;465.
3. Boulter J., O'Shea-Greenfield A., Duvoisin R.M., Connolly J.G., Wada E., Jensen A., Gardner P.D., Ballivet M., Deneris E.S., McKinnon D., Heinemann S., Patrick J. α3, α5, β4: three members of the rat neuronal nicotinic acetylcholine receptor-related gene family form a gene cluster. J. Biol. Chem. 265:1990;4472-4482.
4. Bobbin R.P., Konishi T. Acetylcholine mimics crossed olivocochlear bundle stimulation. Nat. New Biol. 231:1971;222-223.
5. Bobbin R.P., Konishi T. Action of the cholinergic and anticholinergic drugs at the crossed olivocochlear bundle-hair cell junction. Acta Otolarngol. (Stockh.). 77:1974;56-65.
6. Chen C., LeBlanc C., Bobbin R.P. Differences in cholinergic responses form outer hair cells of rat and guinea pig. Hearing Res. 98:1996;9-17.
7. Corriveau R.A., Romano S.J., Conroy W.G., Olivia L., Berg D.K. Expression of neuronal acetylcholine receptor genes in vertebrate skeletal muscle during development. J. Neurosci. 15:1995;1372-1383.
8. Deneris E.S., Boulter J., Swanson W., Patrick J., Heinemann S. β3: a new member of nicotinic acetylcholine receptor gene family is expressed in brain. J. Biol. Chem. 264:1989;6268-6272.
9. Drescher D.G., Khan K.M., Green M.E., Morley B.J., Beisel K.W., Paul J., Gordon D., Gupta A.K., Drescher M.J., Barretto R.L. Nicotinic acetylcholine receptor subunits in the cochlea of the mouse. J. Comp. Biochem. Physiol. 112C:1996;267-273.
10. Elgoyhen A.B., Johnson D.S., Boulter J., Vetter D.E., Heinemann S. α9: An acetylcholine receptor with novel pharmacological properties expressed in rat cochlear hair cells. Cell. 79:1994;705-715.
11. Erostegui C., Norris C.H., Bobbin R.P. In vitro pharmacologic characterization of a cholinergic receptor on outer hair cells. Hearing Res. 74:1994;135-147.
12. Felix D., Ehrenberger K. The efferent modulation of mammalian inner hair cell afferents. Hearing Res. 64:1992;1-5.
13. Forsayeth J.R., Kobrin E., Winegar B.D., Fitzgerald D.J. The nicotinic acetylcholine receptor in cerebellum is composed of four different subunits. Soc. Neurosci. Abstr. 21:1995;1583.
14. Fuchs P.A., Murrow B.W. Cholinergic inhibition of short (outer) hair cells of the chick's cochlea. J. Neurosci. 12:1992;800-809.
15. Gerzanich V., Kuryatov A., Anand R., Lindstrom J. 'Orphan' α6 nicotinic AChR subunit can form a functional heteromeric acetylcholine receptor. Mol. Pharmacol. 51:1997;320-327.
16. Gil-Loyzaga P.E. Neurotransmitters of the olivocochlear lateral efferent system: with an emphasis on dopamine. Acta Otolaryngol. (Stockh.). 115:1995;222-226.
17. Ginzberg R.D., Morest D.K. Fine structure of cochlear innervation in the cat. Hearing Res. 14:1984;109-127.
18. D.A. Godfrey, G.J. Wiet, C.D. Ross, Quantitative histochemistry of the cochlea. In: R.A. Altschuler, R.P. Bobbin, D.W. Hoffman (Eds.), Neurobiology of Hearing: The Cochlea, Raven, New York, 1986, pp. 149-160.
19. Gupta A.K., Chun W.J., Sheikhali S.A., Drescher M.J., Morley B.J., Drescher D.G. Coding sequence of an alpha-9-like nicotinic acetylcholine receptor subunit in a teleost hair cell sheet. Assoc. Res. Otolarngol. Abstr. 20:1997;215.
20. Hiel H., Elgoyhen A., Drescher D.G., Morley B.J. Expression of nicotinic acetylcholine receptor mRNA in the peripheral vestibular system. Brain Res. 738:1996;347-352.
21. Housley G.D., Ashmore J.F. Direct measurement of the action of acetylcholine on isolated outer hair cells of the guinea pig cochlea. Proc. R. Soc. Lond. (Biol.). 264:1991;161-167.
22. Kakehata S., Nakagawa T., Takasaka T., Akaike N. Cellular mechanism of acetylcholine-induced response in dissociated outer hair cells of guinea-pig cochlea. J. Physiol. (Lond.). 463:1993;227-244.
23. Karadaghy A.A., Lasak J.M., Chomchai J.S., Khan K.M., Drescher M.J., Drescher D.G. Quantitative analysis of dopamine receptor messages in the mouse cochlea. Mol. Brain Res. 44:1997;151-156.
24. Kujawa S.G., Glattke T.J., Fallon M., Bobbin R.P. A nicotinic-like receptor mediates suppression of distortion product otoacoustic emissions by contralateral sound. Hearing Res. 74:1994;122-134.
25. Liberman M.C., Dodds L.W., Pierce S. Afferent and efferent innervation of the cat cochlea: quantitative analysis with light and electron microscopy. J. Comp. Neurol. 301:1990;443-460.
26. Listerud M., Brussaard A.B., Devay P., Colman D.R., Role L.W. Functional contribution of neuronal AChR subunits revealed by antisense oligonucleotides. Science. 254:1991;1518-1521.
27. Luebke A.E. Molecular characterization of novel acetylcholine receptors in the guinea pig cochlea. Abstr. Res. Otol. 18:1995;193.
28. Mansour A., Meador-Woodruff J.H., Bunzow J.R., Civelli O., Akil H., Watson S.J. Localization of dopamine D2 receptor mRNA and D1 and D2 receptor binding in the rat brain and pituitary: an in situ hybridization-receptor autoradiographic analysis. J. Neurosci. 10:1995;2587-2600.
29. McGehee D.S., Heath M.J., Gelber S., Devay P., Role L.W. Nicotine enhancement of fast excitatory synaptic transmission in CNS by presynaptic receptors. Science. 269:1995;1692-1696.
30. Merchan-Perez A., Gil-Loyzaga P., Eybalin M., Mateos P.F., Bartolome M.V. Choline-acetyltransferase-like immunoreactivity in the organ of Corti of the rat during postnatal development. Dev. Brain Res. 82:1994;29-34.
31. Morley B.J., Happe H.K. An in situ hybridization study of mRNA expression for α5 and α6, putative nicotinic acetylcholine receptor subunits during postnatal development of the rat brain. Soc. Neurosci. Abstr. 21:1995;1335.
32. B.J. Morley, T. Holderread, A procedure for performing sensitive in situ hybridization on peripheral auditory and vestibular tissues without proteinase K or Triton X-100, Brain Res. Prot., in press.
33. Norris C.H., Guth P.S. The release of acetylcholine (ACh) by the crossed olivo-cochlear bundle (COCB). Acta Otolaryngol. (Stockh.). 77:1974;318-326.
34. Ortells M.O., Lunt G.G. Evolutionary history of the ligand-gated ion-channel superfamily of receptors. Trends Neurosci. 18:1995;121-127.
35. Pujol R. Lateral and medial efferents: a double neurochemical mechanism to protect and regulate inner and outer hair cell function in the cochlea. Br. J. Audiol. 28:1994;185-191.
36. Ramirez-Latorre J.A., Yu C.R., Qu X., Perin F., Karlin A., Role L. Functional contributions of α5 subunit to neuronal acetylcholine receptor channels. Nature. 380:1996;347-351.
37. Robertson D., Johnstone B.M. Efferent transmitter substance in the mammalian cochlea and single neuron support for acetylcholine. Hearing Res. 1:1978;31-34.
38. Seguela P., Wadiche J., Dineley-Miller K., Dani J.A., Patrick J.W. Molecular cloning, functional properties, and distribution of rat brain α7: a nicotinic cation channel highly permeable to calcium. J. Neurosci. 13:1993;596-604.
39. Sobkowicz H.M., Slapnick S.M., Nitecka L.M., August B.K. Compound synapses within the GABAergic innervation of the auditory inner hair cells in the adolescent mouse. J. Comp. Neurol. 377:1997;423-442.
40. Sridhar T.S., Liberman M.C., Brown M.C., Sewell W.F. A novel cholinergic 'slow effect' of efferent stimulation on cochlear potentials in the guinea pig. J. Neurosci. 377:1997;423-442.
41. Vernallis A.B., Conroy W.G., Berg D.K. Neurons assemble acetylcholine receptors with as many as three kinds of subunits while maintaining subunit segregation among receptor subtypes. Neuron. 10:1993;451-464.
42. W.B. Warr, Organization of olivocochlear efferent systems in mammals. In: D.B. Webster (Ed.), Mammalian Auditory Pathways: Neuroanatomy, Raven, New York, pp. 383-396.
43. 2+]i during cell division and differentiation in the mammalian retina. J. Neurosci. 15:1995;2696-2706.
44. Zoli M., Le Novere N., Hill J.A., Changeaux J.-P. Developmental regulation of nicotinic ACh receptor subunit mRNAs in the rat central and peripheral nervous systems. J. Neurosci. 15:1995;1912-1939.