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Volumn 288, Issue 5475, 2000, Pages 2363-2366

Perception of environmental signals by a marine diatom

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CALCIUM HOMEOSTASIS; CARBON DIOXIDE FIXATION; DIATOM; ECOSYSTEM; NONHUMAN; OSMOTIC STRESS; PERCEPTION; PRIORITY JOURNAL; SHEAR STRESS; SIGNAL TRANSDUCTION;

EID: 0034733618     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.288.5475.2363     Document Type: Article
Times cited : (156)

References (33)
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    • note
    • Samples were incubated for 10 min with 0.05% Evans blue (Sigma) and then washed extensively to remove excess and unbound dye. Dye bound to dead cells was solubilized in 50% methanol with 1% sodium dodecyl sulfate for 20 min at 50°C and quantified by absorbance at 600 nm. Under the osmotic conditions tested in Fig. 2, no cell death could be detected.
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    • 0342497530 scopus 로고    scopus 로고
    • For further details, see Web fig. 2 at (4)
    • For further details, see Web fig. 2 at (4).
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    • 84972967695 scopus 로고
    • 3). Experiments were performed only after a late-log-phase culture had passed through at least five successive rounds of growth for 7 days each under different iron-starvation media conditions. All experiments were repeated five times. The amount of dissolved iron (0.2 μm filtered) was determined with graphite furnace atomic absorption spectrophotometry. Measurements of Fe′ were made by means of adsorptive cathodic stripping voltammetry with 2-(2-thiazolylazo)-p-cresol [P. A. M. Farias et al., Anal. Lett. 25, 1929 (1992)].
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    • note
    • We thank R. Riziuto, P. Pinton, J. Alvarez, and C. Neuhaus for advice on calcium calibration; L. Navazio and M. Brini for assistance with the mechanical stimulation experiments; B. van der Wagt (NIOZ) for help with the iron measurements; and T. Wyatt, I. Jenkinson, and C. Brownlee for critical reading of the manuscript. This paper is dedicated to the memory of Professor Gaetano Salvatore.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.