Solution structure of a mutant of transcription factor 1: Implications for enhanced DNA binding

Journal of Molecular Biology

Volumn 302, Issue 4, 2000, Pages 821-830

  Liu, Weidong ^a   Vu, Hai M ^a   Geiduschek, E Peter ^a   Kearns, David R ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Molecular dynamics; NMR; Protein DNA interaction; Transcription factor 1

Indexed keywords

MUTANT PROTEIN; TRANSCRIPTION FACTOR AP 1;

ALPHA HELIX; ARTICLE; BINDING AFFINITY; BINDING SITE; CONFORMATIONAL TRANSITION; DNA BINDING; MOLECULAR DYNAMICS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS; THERMOSTABILITY;

EID: 0034730364     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4084     Document Type: Article

References (43)

1. Determinants of affinity and mode of DNA binding at the carboxy terminus of the bacteriophage SPO1-encoded type II DNA-binding protein, TF1
2. Interrelations of secondary structure stability and DNA-binding affinity in the bacteriophage SPO1-encoded type II DNA-binding protein TF1
3. Structure and dynamics of the DNA binding protein HU from Bacillus stearothermophilus by NMR spectroscopy
4. NMR of Proteins
5. Decreased imino proton exchange and base-pair opening in the IHF-DNA complex measured by NMR
6. Sequence of the bacteriophage SPO1 gene coding for transcription factor 1, a viral homologue of the bacterial type II DNA-binding proteins
7. DNA binding by the bacteriophage SPO1-encoded type II DNA-binding protein, transcription factor 1: Formation of nested complexes at a selective binding site
8. DNA binding by the bacteriophage SPO1-encoded type II DNA-binding protein, transcription factor 1: Site-specific binding requires 5-hydroxymethyluracil-containing DNA
9. Localized DNA flexibility contributes to target site selection by DNA-bending protein
10. On the connection between inherent DNA flexure and preferred binding of hydroxymethyluracil-containing DNA by the type II DNA-binding protein TF1
11. Twin hydroxymethyluracil-A base-pair steps define the binding site for the DNA-bending protein TF1
12. A type II DNA-binding protein genetically engineered for fluorescence spectroscopy: The 'arm' of TF1 binds in the DNA grooves
13. Fluorescence studies of a single tyrosine in a type II DNA binding protein
14. Gradient-enhanced spectroscopy
15. Investigation of exchange processes by two-dimensional NMR spectroscopy
16. Structure of the Bacillus subtilis SPO1-encoded type II DNA-binding protein TF1 in solution
17. Purification of the bacteriophage SPO1 transcription factor 1
18. Practical aspects of 3D heteronuclear NMR of proteins
19. PROCHECK: A program to check the stereochemical quality of protein structures
20. Separation and suppression of coherent transfer effects in two-dimensional NOE and chemical exchange spectroscopy
21. Stereochemical quality of protein structure coordinates
22. 2O
23. Determination of three-dimensional structures of proteins from interproton distance data by dynamical simulated annealing from a random array of atoms
24. The reaction of aromatic peptides with double helical DNA. Quantitative characterization of a two step reaction scheme
25. Making DNA do a U-turn: IHF and related proteins
26. Crystal structure of an IHF-DNA complex: A protein-induced DNA U-turn
27. TF1, the bacteriophage SPO1-encoded type II DNA-binding protein, is essential for viral multiplication
28. Construction and properties of a temperature-sensitive mutation in the gene for the bacteriophage SPO1 DNA-binding protein TF1
29. The effects of mutations at amino acid 61 in the arm of TF1 on its DNA-binding properties
30. DNA-bending properties of TF1
31. Determination of backbone conformation of isotopically enriched proteins based on coupling constants
32. Nuclear magnetic resonance-based model of a TF1/ HmU-DNA complex
33. Structure refined at 2 Å of a nicked DNA octanucleotide complex with DNase I
34. 3 Å Resolution structure of a protein with histone-like properties in prokaryotes
35. DNA recognition by β-sheets
36. DNA-protein interactions: IHF-the master bender
37. Solution structure of the HU protein from Bacillus stearothermophilus
38. NMR study of the interaction of the HU protein from Bacillus stearothermophilus with DNA
39. NMR-derived solution structure of a 17mer hydroxymethyluracil-containing DNA
40. Specificity of hydroxymethyluracil-containing DNA for transcription factor 1: Structural insights
41. 13C NMR chemical shifts and secondary structure analysis
42. A template-selective inhibitor of in vitro transcription