Electroporation of antibodies, DNA, and other macromolecules into cells: A highly efficient method

Journal of Immunological Methods

Volumn 242, Issue 1-2, 2000, Pages 115-126

  Baron, Samuel ^a   Poast, Joyce ^a   Rizzo, Danielle ^b   McFarland, Ellen ^b   Kieff, Elliot ^b  

^a University of Texas Medical Branch School of Medicine   (United States)

^b BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

Antibody; Electroporation; Intracellular; Macromolecules; Virus

Indexed keywords

ANTIBODY; ANTISERUM; DNA; GREEN FLUORESCENT PROTEIN; IMMUNOGLOBULIN G;

ANIMAL CELL; ARTICLE; CANCER CELL; CELL ADHESION; CELL LINE; CELL SURVIVAL; CELL VIABILITY; ELECTROPORATION; HUMAN; HUMAN CELL; MACROMOLECULE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PURIFICATION; SIGNAL TRANSDUCTION; VIRUS CELL INTERACTION;

ANIMAL; ANTIBODIES, VIRAL; CELL LINE; CELL SURVIVAL; CULTURE MEDIA; DNA; ELECTRIC CONDUCTIVITY; ELECTROPORATION; HUMAN; INTRACELLULAR FLUID; LUMINESCENT PROTEINS; MACROMOLECULAR SYSTEMS; RABBITS; SUPPORT, NON-U.S. GOV'T; TIME FACTORS;

EID: 0034726420     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00242-8     Document Type: Article

References (49)

1. Abadia-Molina F., Torreblanca J., Garcia-Herdugo G., Moreno F.J. Inhibition of nucleolar protein nucleolin by electroporation with anti-nucleolin antibodies results in an increase of the nucleolar size. Biol. Cell. 90:1998;355-361.
2. Anderson M.L., Spandidos D.A., Coggins J.R. Electroporation of lymphoid cells: factors affecting the efficiency of transfection. J. Biochem. Biophys. Methods. 22:1991;207-222.
3. Bahnson A.B., Boggs S.S. Addition of serum to electroporated cells enhances survival and transfection efficiency. Biochem. Biophys. Res. Commun. 171:1990;752-757.
4. Baron S., Chopra A.K., Copenhaver D.H., Gelman B.B., Poast J., Singh I.P. A host defense role for a natural antiviral substance in the nervous system. J. Neuroimmunol. 85:1998;168-173.
5. Baron S., Poast J. Intracellular IFN is not antiviral and intracellular antibody to IFN does not inhibit IFN's action. Eur. Cytokine Netw. 7:1996;518.
6. Baron S., Poast J., Cloyd M.W. Why is HIV rarely transmitted by oral secretions? Saliva can disrupt orally shed, infected leukocytes. Arch. Intern. Med. 159:1999;303-310.
7. Barry E.L.R., Gesek F.A., Friedman P.A. Introduction of antisense oligonucleotides into cells by permeabilization with streptolysin O. Biotechniques. 15:1993;1016-1020.
8. Baum C., Forster P., Hegewisch-Becker S., Harbers K. An optimized electroporation protocol applicable to a wide range of cell lines. Biotechniques. 17:1994;1058-1062.
9. Berglund D.L., Starkey J.R. Isolation of viable tumor cells following introduction of labelled antibody to an intracellular oncogene product using electroporation. J. Immunol. Methods. 125:1989;79-87.
10. Biocca S., Pierandrei-Amaldi P., Campioni N., Cattaneo A. Intracellular immunization with cytosolic recombinant antibodies. Biotechnology. 12:1994;396-399.
11. Boes R., Obe G. Scrape-loading: A simple method to induce chromosomal aberrations with restriction enzymes in CHO cells. Mutat. Res. 292:1993;225-230.
12. Coonrod A., Li F.Q., Horwitz M. On the mechanism of DNA transfection: efficient gene transfer without viruses. Gene Ther. 4:1997;1313-1321.
13. Fakhrai H., Dorigo O., Shawler D.L., Lin H., Mercola D., Black K.L., Royston I., Sobol R.E. Eradication of established intracranial rat gliomas by transforming growth factor β antisense gene therapy. Proc. Natl. Acad. Sci. USA. 93:1996;2909-2914.
14. Fechheimer M., Boylan J.F., Parker S., Sisken J.E., Patel G.L., Zimmer S.G. Transfection of mammalian cells with plasmid DNA by scrape loading and sonication loading. Proc. Natl. Acad. Sci. USA. 84:1987;8463-8467.
15. Harding C.V. III. Electroporation of exogenous antigen into the cytosol for antigen processing and class I major histocompatibility complex (MHC) presentation: weak base amines and hypothermia (18°C) inhibit the class I MHC processing pathway. Eur. J. Immunol. 22:1992;1865-1869.
16. Hejna J.A., Johnstone P.L., Kohler S.L., Bruun D.A., Reifsteck C.A., Olson S.B., Moses R.E. Functional complementation by electroporation of human BACs into mammalian fibroblast cells. Nucleic Acids Res. 26:1998;1124-1125.
17. Heller R., Jaroszeski M., Atkin A., Moradpour D., Gilbert R., Wands J., Nicolau C. In vivo gene electroinjection and expression in rat liver. FEBS Lett. 389:1996;225-228.
18. Hughes T.K., Blalock J.E., McKerlie M.L., Baron S. Cell-produced viral inhibitor: possible mechanism of action and chemical composition. Infect. Immun. 32:1981;454-457.
19. Kumar S., McKerlie M.L., Albrecht T.B., Goldman A.S., Baron S. A broadly active viral inhibitor in human and animal organ extracts and body fluids (41918). Proc. Soc. Exp. Biol. Med. 177:1984;104-111.
20. Lafon M., Lafage M. Antiviral activity of monoclonal antibodies specific for the internal proteins N and NS of rabies virus. J. Gen. Virol. 68:1987;3113-3123.
21. Lee G., Delohery T.M., Ronai Z., Brandt-Rauf P.W., Pincus M.R., Murphy R.B., Weinstein I.B. A comparison of techniques for introducing macromolecules into living cells. Cytometry. 14:1993;265-270.
22. Lewis R. Electroporation edges toward clinic for both gene therapy and drug delivery. Genet. Eng. News. 19:1997;37.
23. Liu S.H., Chu J.C., Ng S.Y. Streptolysin O permeabilized cell system for studying transacting activities of exogenous nuclear proteins. Nucleic Acids Res. 21:1993;4005-4010.
24. Lyerly H.K., DiMaio J.M. Gene delivery systems in surgery. Arch. Surg. 128:1993;1197-1206.
25. Matthews K.E., Dev S.B., Toneguzzo F., Keating A. Electroporation for gene therapy. Nickoloff J.A. Methods in Molecular Biology: Animal Cell Electroporation and Electrofusion Protocols. Vol. 48:1995;273-280 NJ, Totowa.
26. McNeil P.L., Murphy R.F., Lanni F., Taylor D.L. A method for incorporating macromolecules into adherent cells. J. Cell Biol. 98:1984;1556-1564.
27. Mir L.M., Banoun H., Paoletti C. Introduction of definite amounts of nonpermeant molecules into living cells after electropermeabilization: Direct access to the cytosol. Exp. Cell Res. 175:1988;15-25.
28. Mitalipov S.M., White K.L., Farrar V.R., Morrey J., Reed W.A. Development of nuclear transfer and parthenogenetic rabbit embryos activated with inositol 1,4,5-trisphosphate. Biol. Reprod. 60:1999;821-827.
29. Montano X., Jimenez A. Intracellular expression of the monoclonal anti-ras antibody Y13-259 blocks the transforming activity of ras oncogenes. Cell Growth Differ. 6:1995;597-605.
30. Neuman E., Schaefer-Ridder M., Wang Y., Hofschneider P.H. Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J. 1:1982;841-845.
31. Nishi T., Yoshizato K., Yamashiro S., Takeshima H., Sato K., Hamada K., Kitamura I., Yoshimura T., Saya H., Kuratsu J.-I., Ushio Y. High-efficiency in vivo gene transfer using intraarterial plasmid DNA injection following in vivo electroporation. Cancer Res. 56:1996;1050-1055.
32. Nomura M., Nakata Y., Inoue T., Uzawa A., Itamura S., Nerome K., Akashi M., Suzuki G. In vivo induction of cytotoxic T lymphocytes specific for a single epitope introduced into an unrelated molecule. J. Immunol. Methods. 193:1996;41-49.
33. Orrantia E., Chang P.L. Intracellular distribution of DNA internalized through calcium phosphate precipitation. Exp. Cell Res. 190:1990;170-174.
34. Ortiz D., Baldwin M.M., Lucas J.J. Transient correction of genetic defects in cultured animal cells by introduction of functional proteins. Mol. Cell Biol. 7:1987;3012-3017.
35. Pardridge W.M., Kang Y.S., Yang J., Buciak J.L. Enhanced cellular uptake and in vivo biodistribution of a monoclonal antibody following cationization. J. Pharm. Sci. 84:1995;943-948.
36. Potter H., Weir L., Leder P. Enhancer-dependent expression of human κ immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation. Proc. Natl. Acad. Sci. USA. 81:1984;7161-7165.
37. Richardson J.H., Marasco W.A. Intracellular antibodies: Development and therapeutic potential. Trends Biotechnol. 13:1995;306-310.
38. Schulz I. Permeabilizing cells: Some methods and applications for the study of intracellular processes. Methods Enzymol. 192:1990;280-300.
39. Shen Y.M., Hirschhorn R.R., Mercer W.E., Surmacz E., Tsutsui Y., Soprano K.J., Baserga R. Gene transfer: DNA microinjection compared with DNA transfection with a very high efficiency. Mol. Cell. Biol. 2:1982;1145-1154.
40. Smith C. Retroviral vector mediated gene transfer into hematopoietic cells: prospects and issues. J. Hematother. 1:1992;155-166.
41. Smith M.R., Muegge K., Keller J.R., Kung H.F., Young H.A., Durum S.K. Direct evidence for an intracellular role for IFN-gamma. Microinjection of human IFN-gamma induces Ia expression on murine macrophages. J. Immunol. 144:1990;1777-1782.
42. Smith M.R., Munger W.E., Kung H.F., Takacs L., Durum S.K. Direct evidence for an intracellular role for tumor necrosis factor-alpha 1. Microinjection of tumor necrosis factor kills target cells. J. Immunol. 144:1990;162-169.
43. Termignon J.L., Pradier F., Petit A., Journois D., Weiss M., Mazmanian M. Effects of calcium concentration, lactobionate content, and sodium/potassium ratio of preservation solutions on resting left ventricular pressure and postreperfusion function of rabbit heterotopic heart transplants. J. Heart Lung Transplant. 14:1995;1126-1135.
44. Tewari D., Goldstein S.L., Notkins A.L., Zhou P. cDNA encoding a single-chain antibody to HIV p17 with cytoplasmic or nuclear retention signals inhibits HIV-1 replication. J. Immunol. 161:1998;2642-2647.
45. Toneguzzo F., Hayday A.C., Keating A. Electric field-mediated DNA transfer: transient and stable gene expression in human and mouse lymphoid cells. Mol. Cell Biol. 6:1986;703-706.
46. Wigler M., Pellicer A., Silverstein S., Axel R., Urlaub G., Chasin L. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells. Proc. Natl. Acad. Sci. USA. 76:1979;1373-1376.
47. Zerbib D., Amalric F., Teissie J. Electric field mediated transformation: isolation and characterization of a TK+ subclone. Biochem. Biophys. Res. Commun. 129:1985;611-618.
48. + cells transfected with IL-16 cDNA are resistant to HIV-1 infection: Inhibition of mRNA expression. Nat. Med. 3:1997;659-664.
49. Zhou P., Goldstein S., Devadas K., Tewari D., Notkins A.L. Cells transfected with a non-neutralizing antibody gene are resistant to HIV infection: Targeting the endoplasmic reticulum and transgolgi network. J. Immunol. 160:1998;1489-1496.