Inhibition of nucleolar protein nucleolin by electroporation with anti-nucleolin antibodies results in an increase of the nucleolar size

Biology of the Cell

Volumn 90, Issue 4, 1998, Pages 355-361

  Abadía Molina, Francisco ^b   Torreblanca, José ^a   García Herdugo, Gregorio ^a   Moreno, Francisco J ^a  

^a UNIVERSITY OF SEVILLE   (Spain)

^b NONE

Author keywords

C23; Electroporation; Image analysis; Nucleolin; Nucleolus

Indexed keywords

AUTOANTIBODY; NUCLEAR PROTEIN; NUCLEOLIN; RIBOSOME RNA;

ARTICLE; CANCER CELL CULTURE; CONTROLLED STUDY; ELECTRON MICROSCOPY; ELECTROPORATION; HUMAN; HUMAN CELL; IMAGE ANALYSIS; LARYNX CARCINOMA; MOLECULAR BIOLOGY; NUCLEOLUS; PRIORITY JOURNAL; RNA PROCESSING; SQUAMOUS CELL CARCINOMA; TIME;

EID: 0032456062     PISSN: 02484900     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0248-4900(98)80016-6     Document Type: Article

References (47)

1. cdc2 protein kinase. Mol Cell Biol 10, 3607-3618
2. Bendayan M, Roth J, Perrelet A and Orci L (1980) Quantitative immunocytochemical localization of pancreatic secretory proteins in subcellular compartments of the rat acinar cell. J Histochem Cytochem 28, 149-160
3. Borer RA, Lehner CF, Eppenberger HN and Nigg EA (1989) Major nucleolar proteins shuttle between nucleus and cytoplasm. Cell 56, 379-390
4. Bouche G, Caizergues-Ferrer M, Bugler B and Amalric F (1984) Interrelations between the maturation of the 100 kDa nucleolar protein and pre-rRNA synthesis in CHO cells. Nucleic Acids Res 12, 3025-3035
5. Bugler B, Caizergues-Ferrer M, Bouche G, Bourbon H and Amalric F (1982) Detection and localization of a class of proteins immunologically related to a 100-kDa nucleolar protein. Eur J Biochem 128, 475-480
6. Bugler B, Bourbon HM, Lapeyre B, Wallace M, Chang JH, Amalric F and Olson MOJ (1987) RNA binding fragments from nucleolin contain the ribonucleoprotein consensus sequence. J Biol Chem 262, 10922-10925
7. Caizergues-Ferrer M, Belenguer P, Lapeyre B, Amalric F, Wallace MO and Olson MOJ (1987) Phosphorylation of nucleolin by a nucleolar type NII protein kinase. Biochemistry 26, 7876-7883
8. Chen CM, Chiang SY and Yeh NH (1991) Increased stability of nucleolin in proliferating cells by inhibition of its self-cleaving activity. J Biol Chem 266, 7754-7758
9. Créancier L, Prats H, Zanibellato C, Amalric F and Bugler B (1993) Determination of the functional domains involved in nucleolar targeting of nucleolin. Mol Biol Cell 4, 1239-1250
10. Csermely P, Schnaider T, Cheatman B, Olson MOJ and Kahn CR (1993) Insulin induces the phosphorilation of nucleolin. J Biol Chem 268, 9747-9752
11. Erard M, Belenguer P, Caizergues-Ferrer M, Pantaloni A and Amalric F (1988) A major nucleolar protein, nucleolin, induces chromatin decondensation by binding to histone H1. Eur J Biochem 175, 525-530
12. Erard M, Lakhdar-Ghazal F and Amalric F (1990) Repeat peptide motifs which contain β-turns and modulate DNA condensation in chromatin. Eur J Biochem 191, 19-26
13. Escande ML, Gas N and Stevens BJ (1985) Immunolocalization of the 100 KDa nucleolar protein in CHO cells. Biol Cell 53, 99-109
14. Fang SH and Yeh NH (1993) The self-cleaving activity of nucleolin determines its molecular dynamics in relation to cell proliferation. Exp Cell Res 208, 48-53
15. Gas N, Escande ML and Stevens BJ (1985) Immunolocalization of the 100 kDa nucleolar protein during the mitotic cycle in CHO cells. Biol Cell 53, 209-218
16. Ginisty H, Amalric F And Bouvet P (1998) Nucleolin functions in the first step of ribosomal RNA processing. EMBO J 17, 1476-1486
17. Goessens G (1984) Nucleolar structure. Int Rev Cyt 87, 107-158
18. Guetzow K and Busch H (1981) Localization of phosphoprotein C23 in nucleoli by immunological methods. Exp Cell Res 135, 259-265
19. Gulli MP, Girard JP, Zabetakis D, Lapeyre B, Melese T and Caizergues-Ferrer M (1995) Gar2 is a nucleolar protein from Schizosaccaromyces pombe required for 18S rRNA and 40S ribosomal subunit accumulation. Nucleic Acids Res 23, 1912-1918
20. Hadjiolov AA (1985) The nucleolus and ribosome biogenesis. Cell Biol Monogr 12, 1-268
21. Hanakahi LA, Dempsey LA, Li MJ and Maizels N (1997) Nucleolin is one component of the B cell-specific transcription factor and a scwitch region binding protein, LR1. Proc Natl Acad Sci USA 94, 3605-3610
22. Heine MA, Rankin ML and DiMario PJ (1993) The Gly/Argrich (GAR) domain of Xenopus nucleolin facilitates in vitro nucleic acid binding and in vivo nucleolar localization. Mol Biol Cell 4, 1189-1204
23. Herrera AH and Olson MOJ (1986) Association of protein C23 with rapidly labeled nucleolar RNA. Biochemistry 25, 6558-6264
24. Jordan EG and Cullis CA (eds) (1982) The Nucleolus. Cambridge University Press, Cambridge
25. Kharrat A, Derancourt J, Doree M, Amalric F and Erard M (1991) Synergistic effect of histone H1 and nucleolin on chromatin condensation in mitosis: role of a phosphorylated heteromer. Biochemistry 30, 10329-10336
26. Kondo K and Inouye M (1992) Yeast NSR1 protein that has structural similarity to mammalian nucleolin is involved in pre-rRNA processing. J Biol Chem 267, 16252-16258
27. Kondo K, Zowalski LRZ and Inouye M (1992) Cold shock induction of yeast NSR1 protein and its role in pre-rRNA processing. J Biol Chem 267, 16259-16265
28. Lapeyre B, Bourbon H and Amalric F (1987) Nucleolin, the major nucleolar protein of growing eukaryotic cells: an unusual protein structure revealed by the nucleotide sequence. Proc Natl Acad Sci USA 84, 1472-1476
29. Lee WC, Zabetakis D and Mélèse T (1992) NSR1 is required for pre-rRNA processing and for the proper maintenance of steady-state levels of ribosomal subunits. Mol Cell Biol 12, 3865-3871
30. Lischwe MA, Richards RL, Busch RK and Busch H (1981) Localization of phosphoprotein C23 to nucleolar structures and to the nucleolus organizer region. Exp Cell Res 136, 101-109
31. Moreno FJ, Hernandez-Verdun D, Masson C and Bouteille M (1985) Silver staining of the nucleolar organizer regions (NORs) on Lowicryl and cryo-ultrathin sections. J Histochem Cytochem 33, 389-399
32. Olson MOJ, Orrick LR, Olson MOJ and Bush H (1973) Comparison of nucleolar proteins of normal rat liver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis. Proc Natl Acad Sci USA 70, 1316-1320
33. Olson MOJ, Rivers ZM, Thompson BA, Kao WI and Case ST (1983) Interaction of nucleolar phosphoprotein C23 with cloned segments of rat ribosomal deoxyribonucleic acid. Biochemistry 22, 3345-3351
34. Olson MOJ (1990) The role of proteins in nucleolar structure and function. In: The Eukaryotic Nucleus: Molecular Biochemistry and Macromolecular Assemblies (Straus PR and Wilson SH, eds) The Telford Press Inc, Caldwell MJ, Vol 2, 519-559
35. Peter M, Nakagawa J, Dorée M, Labbé JC and Nigg EA (1990) Identification of major nucleolar proteins as candidate mitotic substrates of cdc2 kinase. Cell 60, 791-801
36. Rendon MC, Rodrigo RM, Goenechea LG, Garcia-Herdugo G, Vadivia MM and Moreno FJ (1992) Characterization and immunolocalization of a nucleolar antigen with anti-NOR serum in HeLa cells. Exp Cell Res 200, 393-403
37. Rodrigo RM, Torreblanca J, García-Herdugo G and Moreno FJ (1994) An experimental approach to the study of Ag-NOR proteins. Acta Histochem Cytochem 27, 259-273
38. Roth J (1983) Application of lectin-gold complexes for electron microscopic localization of glycoconjugates of thin sections. J Histochem Cytochem 200, 897-999
39. Roth J, Taatjes DJ and Warhol MJ (1989) Prevention of nonspecific interactions of gold-labeled reagents on tissue sections. Histochemistry 92, 47-56
40. Schneider HR and Issinger OG (1988) Nucleolin (C23), a physiological substrate for casein kinase II. Biochem Biophys Res Commun 156, 1390-1397
41. Serin G, Joseph G, Faucher C, Ghisolfi L, Bouche G, Amalric F and Bouvet P (1996) Localization of nucleolin binding sites on human and mouse pre-ribosomal RNA. Biochemie 78, 530-538
42. Serin G, Joseph G, Ghisolfi L, Bauzan M, Erard M, Amalric F and Bouvet P (1997) Two RNA-binding domains determine the RNA-binding specificity of nucleolin. J Biol Chem 272, 13109-13116
43. Sommerville J (1986) Nucleolar structure and ribosome biogenesis. Trends Biochem Sci 11, 438-442
44. Spector DL, Ochs RL and Busch H (1984) Silver staining, immunofluorescence and immunoelectron microscopic localization of nucleolar phosphoproteins B23 and C23. Chromosoma 90, 139-148
45. Tawfic S, Goueli SA, Olson MO and Ahmed K (1994) Androgenic regulation of phosphorilation and stability of nucleolar protein nucleolin in rat ventral prostate. Prostate 24, 101-106
46. Trapman J, Retel J and Planta RJ (1975) Ribosomal precursor particles from yeast. Exp Cell Res 90, 95-104
47. Tujeta N, Huang NW, Skopac D, Tujeta R, Hrvatic S, Zhang J, Pongor S, Joseph G, Fauncher F, Amalric F and Falaschi A (1995) Human DNA helicase IV is nucleolin, an RNA helicase modulated by phosphorylation. Gene 160, 143-148