Studies of UDP-galactopyranose mutase from Escherichia coli: An unusual role of reduced FAD in its catalysis

Journal of the American Chemical Society

Volumn 122, Issue 38, 2000, Pages 9065-9070

  Zhang, Qibo ^a   Liu, Hung Wen ^a,b  

^a University of Minnesota   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLAVINE ADENINE NUCLEOTIDE; MUTASE; SODIUM DITHIONITE; URIDINE DIPHOSPHATE;

ARTICLE; CATALYSIS; COENZYME; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME MECHANISM; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME SYNTHESIS; EQUILIBRIUM CONSTANT; ESCHERICHIA COLI; NONHUMAN; OXIDATION REDUCTION REACTION;

ESCHERICHIA COLI;

EID: 0034721448     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja001333z     Document Type: Article

References (34)

1. Trejo, A. G.; Chittenden, G. J. F.; Buchanan, J. G.; Baddiley, J. Biochem. J. 1970, 117, 637-639.
2. (a) de Lederkremer, R. M.; Colli, W. Glycobiology 1995, 5, 547-552.
3. (b) Brennan, P. J.; Nikaido, H. Annu. Rev. Biochem. 1995, 64, 29-63.
4. (c) Whitfield, C. Trends Microbiol. 1995, 3, 178-185.
5. (d). Previato, J. O.; Mendonca-Previato, L.; Jones, C.; Wait, R. Glycoconjugate J. 1993, 10, 340.
6. Nassau, P. M.; Martin, S. L.; Brown, R. E.; Weston, A.; Monsey, D.; McNeil, M. R.; Duncan, K. J. Bacteriol. 1996, 178, 1047-1052.
7. (a) Koplin, R.; Brisson, J.-R.; Whitfield, C. J. Biol. Chem. 1997, 272, 4121-4128.
8. (b) Weston, A.; Stern, R. J.; Lee, R. E.; Nassau, P. M.; Monsey, D.; Martin, S. L.; Scherman, M. S.; Besra, G. S.; Duncan, K.; McNeil, M. R. Tuberc. Lung Dis. 1998, 78, 123-131.
9. (c) Chen, P.; Bishai, W. Infect. Immun. 1998, 66, 5099-5106.
10. (d) McMahon, S. A.; Leonard, G. A.; Buchanan, L. V.; Giraud, M.-F.; Naismith, J. H. Acta Crystallogr. 1999, D55, 399-402.
11. Barlow, J. N.; Girvin, M. E.; Blanchard, J. S. J. Am. Chem. Soc. 1999, 121, 6968-6969.
12. The wild-type enzyme was reported to be unstable even in the presence of glycerol. 3
13. Lee, R.; Monsey, D.; Weston, A.; Duncan, K.; Rithner, C.; McNeil, M. Anal. Biochem. 1996, 242, 1-7.
14. Ovensky, J.; McNeil, M.; Sinay, P. J. Org. Chem. 1999, 64, 6202-6205.
15. de Lederkremer, R. M.; Nahmad, V. B.; Varela, O. J. Org. Chem. 1994, 59, 690-692.
16. de Lederkremer, R. M.; Litter, M. I. Carbohydr. Res. 1971, 20, 442-444.
17. Kohn, P.; Samaritano, R. H.; Lerner, L. M. J. Am. Chem. Soc. 1965, 87, 5475-5480.
18. D'Accorso, N. B.; Thiel, I. M. E. Carbohydr. Res. 1983, 124, 177-184.
19. Chen, H.; Guo, Z.; Liu, H.-w. J. Am. Chem. Soc. 1998, 120, 11796-11797.
20. NAD(P)H was reported to be required for the activity of mutase isolated from Klebsiella pneumoniae. 4a
21. Riddles, P. W.; Blakeley, R. L.; Zerner, B. Methods Enzymol. 1983, 91, 49-60.
22. The mutase from Mycobacterium tuberculosis contains no cysteine residues at all, 4b and that from Klebsiella pneumoniae serotype O1 contains six cysteines. 4a Since these cysteine residues are not conserved, they are unlikely to play active roles in the proposed mechanism.
23. Massey, V.; Hemmerich, P. Biochemistry 1978, 17, 9-17.
24. Kerwin, J. F. Jr.; Lancaster, J. R., Jr.; Feldman, P. L. J. Med. Chem. 1995, 38, 4343-4362.
25. Chipman, D.; Barak, Z.; Schloss, J. V. Biochim. Biophys. Acta 1998, 1385, 401-419.
26. Xu, L.-L.; Singh, B. K.; Conn, E. E. Arch. Biochem. Biophys. 1986, 250, 322-328.
27. Cromartie, T. H.; Walsh, C. T. J. Biol. Chem. 1976, 251, 329-333.
28. Macheroux, P.; Schmid, J.; Amrhein, N.; Schaller, A. Planta 1999, 207, 325-334 and references therein.
29. Zhang, Q.; Cone, M. C.; Gould, S. J.; Zabriskie, T. M. Tetrahedron 2000, 56, 693-701.
30. Sambrook, J.; Fritsch, E. F.; Maniatis, T. Molecular Cloning: A Laboratory Manual, 2nd ed.; Cold Spring Harbor Laboratory Press: Spring Harbor, NY, 1989.
31. Bradford, M. M. Anal. Biochem. 1976, 72, 248-254.
32. Laemmli, U. K. Nature 1970, 227, 680-685.
33. Andrews, P. Biochem. J. 1964, 91, 222-223.
34. Ashton, W. T.; Brown, R. D.; Tolman, R. L. J. Heterocycl. Chem. 1978, 15, 489-491.