Positional isotope exchange catalyzed by UDP-galactopyranose mutase [17]

Journal of the American Chemical Society

Volumn 121, Issue 29, 1999, Pages 6968-6969

  Barlow, John N ^a   Girvin, Mark E ^a   Blanchard, John S ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON NUCLEAR MAGNETIC RESONANCE; CATALYSIS; ENZYME MECHANISM; ISOTOPE LABELING; LETTER; REACTION ANALYSIS;

EID: 0033612742     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja991582r     Document Type: Letter

References (18)

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2. (b) Brennan, P. J.; Nikaido, H. Annu. Rev. Biochem. 1995, 64, 29.
3. (c) de Lederkremer, R. M.; Colli, W. Glycobiology 1995, 5, 547.
4. (a) Köplin, R.; Brisson, J.-R.; Whitfield, C. J. Biol. Chem. 1997, 272, 4121.
5. (b) Nassau P. M.; Martin, S. L.; Brown, R. E.; Weston, A.; Monsey, D.; McNeil, M. R.; Duncan, K. J. Bacteriol. 1996, 178, 1047.
6. (c) Lee, R.; Monsey, D.; Weston, A.; Duncan, K.; Rithner, C.; McNeil, M. Anal. Biochem. 1996, 242, 1.
7. (d) McMahon, S. A.; Leonard, G. A.; Buchanan, L. V.; Giraud, M.-F.; Naismith, J. H. Acta Crystallogr. 1999, D55, 399
8. Barlow, J. N.; Marcinkeviciene, J.; Blanchard, J. S. In Enzymatic Mechanisms; Frey, P. A., Northrop, D. B., Eds.; IOS Press, 1999; p 98.
9. Raushel, F. M.; Villafranca, J. J. CRC Crit. Rev. Biochem. 1988, 23, 1.
10. (a) Villafranca, J. J. Methods Enzymol. 1989, 177, 390
11. (b) Sala, R. F.; Morgan, P. M.; Tanner, M. E. J. Am. Chem. Soc. 1996, 118, 3033.
12. 18O)-1-phosphate and uridylation by galactose-1-phosphate-uridyl- transeferase and UDP-glucose to give 1 was performed in a one-pot reaction. 1 was purified by Mono-Q anion-exchange and P-2 biogel fast performance liquid chromatography (FPLC). Mass analysis by electrospray ionization mass spectrometry (negative mode) gave a mass of 568.0 Da (predicted mass for monoanion = 568.1 Da).
13. 18O)-1-phosphate and uridylation by galactose-1-phosphate-uridyl- transeferase and UDP-glucose to give 1 was performed in a one-pot reaction. 1 was purified by Mono-Q anion-exchange and P-2 biogel fast performance liquid chromatography (FPLC). Mass analysis by electrospray ionization mass spectrometry (negative mode) gave a mass of 568.0 Da (predicted mass for monoanion = 568.1 Da).
14. 18O)-1-phosphate and uridylation by galactose-1-phosphate-uridyl-transeferase and UDP-glucose to give 1 was performed in a one-pot reaction. 1 was purified by Mono-Q anion-exchange and P-2 biogel fast performance liquid chromatography (FPLC). Mass analysis by electrospray ionization mass spectrometry (negative mode) gave a mass of 568.0 Da (predicted mass for monoanion = 568.1 Da).
15. (a) Risky, J. M.; Van Etten, R. L. Methods Enzymol. 1989, 177, 376.
16. (b) Risley, J. M.; Kuo, F.; Van Etten, R. L. J. Am. Chem. Soc. 1983, 105, 1647.
17. Recombinant UDP-galp mutase from Klebsiella pneumoniae was purified from E. coli. Details of the cloning, expression, and purification of the enzyme will be published separately.
18. The method from ref 2c was used.