Measurement of absolute concentration and viability of CD34+ cells in cord blood and cord blood products using fluorescent beads and cyanine nucleic acid dyes

Communications in Clinical Cytometry

Volumn 34, Issue 3, 1998, Pages 121-127

  Hübl, Wolfgang ^a   Iturraspe, Jose ^b   Martinez, Guillermo A ^b   Hutcheson, Charles E ^a   Roberts, Cheryl G ^a   Fisk, Diann D ^a   Sugrue, Michelle W ^a   Wingard, John R ^a   Braylan, Raul C ^a  

^a UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

^b LifeSouth Community Blood Centers   (United States)

Author keywords

CD34; Cord blood; Flow cytometry; Hematopoietic stem cells

Indexed keywords

CYANINE DYE;

ARTICLE; CELL COUNT; CELL VIABILITY; FLUORESCENCE; HUMAN; HUMAN CELL; LYMPHOCYTE SUBPOPULATION; PRIORITY JOURNAL; UMBILICAL CORD BLOOD;

EID: 0031826706     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19980615)34:3<121::AID-CYTO2>3.3.CO;2-U     Document Type: Article

References (27)

1. Almici C, Carlo-Stella C, Wagner JE, Mangoni L, Garau D, Rizzoli V: Biologic and phenotypic analysis of early hematopoietic progenitor cells in umbilical cord blood. Leukemia 11:2143-2149, 1997.
2. + marrow cells, devoid of T and B lymphocytes, reconstitute stable lymphopoiesis and myelopoiesis in lethally irradiated allogeneic baboons. Blood 80:1693-1701, 1992.
3. Bender JG, To LB, Williams S, Schwartzberg LS: Defining a therapeutic dose of peripheral blood stem cells. J Hematother 1:329-341, 1992.
4. Cairo MS, Wagner JE: Placental and/or umbilical cord blood: An alternative source of hematopoietic stem cells for transplantation. Blood 90:4665-4678, 1997.
5. + cell analysis by flow cytometry: The ISHAGE guidelines. Clin Immun Newsletter 17:21-42, 1997.
6. Fritsch G, Printz D, Stimpfl M, Dworzak MN, Witt V, Potschger U, Buchinger P: Quantification of CD34+ cells: Comparison of methods. Transfusion 37:775-784, 1997.
7. Fritsch G, Stimpfl M, Kurz M, Printz D, Buchinger P, Fischmeister G, Hoecker P, Gadner H: The composition of CD34 subpopulations differs between bone marrow, blood and cord blood. Bone Marrow Transplant 17:169-178, 1996.
8. Hao QL, Shah AJ, Thiemann FT, Smogorzewska EM, Crooks GM: A functional comparison of CD34 + CD38- cells in cord blood and bone marrow. Blood 86:3745-3753, 1995.
9. Idziorek T, Estaquier J, De BF, Ameisen JC: YOPRO-I permits cytofluorometric analysis of programmed cell death (apoptosis) without interfering with cell viability. J Immunol Methods 185:249-258, 1995.
10. + cell counts based on the ISHAGE guidelines. Cytometry 34:61-70, 1998.
11. Krause DS, Fackler MJ, Civin CI, May WS: CD34: Structure, biology, and clinical utility. Blood 87:1-13, 1996.
12. Lansdorp PM, Dragowska W, Mayani H: Ontogeny-related changes in proliferative potential of human hematopoietic cells. J Exp Med 178:787-791, 1993.
13. Linnet K Evaluation of regression procedures for methods comparison studies. Clin Chem 39:424-432, 1993.
14. Passing H, Bablok W: A new biometrical procedure for testing the equality of measurements from two different analytical methods. Application of linear regression procedures for method comparison studies in clinical chemistry, Part I. J Clin Chem Clin Biochem 21:709-720, 1983.
15. Passing H, Bablok W: Comparison of several regression procedures for method comparison studies and determination of sample sizes. Application of linear regression procedures for method comparison studies in Clinical Chemistry, Part II. J Clin Chem Clin Biochem 22:431-445, 1984.
16. Rubinstein P, Dobrila L, Rosenfield RE, Adamson JW, Migliaccio G, Migliaccio AR, Taylor PE, Stevens CE: Processing and cryopreservation of placental/umbilical cord blood for unrelated bone marrow reconstitution. Proc Natl Acad Sci USA 92:10119-10122, 1995.
17. Rubinstein P, Rosenfield RE, Adamson JW, Stevens CE: Stored placental blood for unrelated bone marrow reconstitution. Blood 81:1679-1690, 1993.
18. Savage RA: Pseudoleukocytosis due to EDTA-induced platelet clumping. Am J Clin Pathol 81:317-322, 1984.
19. Shpall EJ, Jones RB, Bearman SI, Franklin WA, Archer PG, Curiel T, Bitter M, Claman HN, Stemmer SM, Purdy M: Transplantation of enriched CD34-positive autologous marrow into breast cancer patients following high-dose chemotherapy: Influence of CD34-positive peripheral-blood progenitors and growth factors on engraftment. J Clin Oncol 12:28-36, 1994.
20. Sims LC, Brecher ME, Gertis K, Jenkins A, Nickischer D, Schmitz JL, Sparks S, Wright P, Bentley SA: Enumeration of CD34-positive stem cells: Evaluation and comparison of three methods. J Hematother 6:213-226, 1997.
21. Statland BE, Winkel P, Harris SC, Burdsall MJ, Saunders AM: Evaluation of biologic sources of variation of leukocyte counts and other hematologic quantities using very precise automated analyzers. Am J Clin Pathol 69:48-54, 1978.
22. + cells from umbilical cord blood, granulocyte colony-stimulating factor-mobilized peripheral blood, and adult human bone marrow. J Hematother 3:253-262, 1994.
23. + cell determination by flow cytometry. International Society of Hematotherapy and Graft Engineering. J Hematother 5:213-226, 1996
24. Sutherland DR, Keating A: The CD34 antigen: Structure, biology, and potential clinical applications. J Hematother 1:115-129, 1992.
25. Tricot G, Jagannath S, Vesole D, Nelson J, Tindle S, Miller L, Cheson B, Crowley J, Barlogie B: Peripheral blood stem cell transplants for multiple myeloma: Identification of favorable variables for rapid engraftment in 225 patients. Blood 85:588-596, 1995.
26. Van Hooijdonk CAEM, Glade CP, Van Erp PEJ: TO-PRO-3 iodide: A novel HeNe laser-excitable DNA stain as an alternative for propidium iodide in multiparameter flow cytometry. Cytometry 17:185-189, 1994.
27. + cord blood cells: Comparative analysis by flow cytometry and limiting dilution (LD) RT-PCR of chymopapain-treated or untreated cells. Cytometry 25:46-57, 1996.