High-resolution in vivo footprinting of a protein - DNA complex using γ-radiation [13]

Journal of the American Chemical Society

Volumn 122, Issue 24, 2000, Pages 5901-5902

  Ottinger, Lori M ^a   Tullius, Thomas D ^a  

^a Boston University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; SINGLE STRANDED DNA;

BINDING SITE; DNA FOOTPRINTING; DNA PROTEIN COMPLEX; GAMMA RADIATION; IONIZING RADIATION; LETTER; PROTEIN DNA BINDING;

EID: 0034697671     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja000285f     Document Type: Letter

References (22)

1. Tullius, T. D. Annu. Rev. Biophys. Biophys. Chem. 1989, 18, 213-237.
2. (a) Becker, P. B.; Weih, F.; Schutz, G. Methods Enzymol. 1993, 218, 568-587.
3. (b) Warshawsky, D.; Miller, L. Methods Mol. Biol. 1999, 127, 199-212.
4. Simpson, R. T. Curr. Opin. Genet. Devel. 1999, 9, 225-229.
5. Tullius, T. D.; Dombroski, B. A. Proc. Natl. Acad. Sci. U.S.A. 1986, 83, 5469-5473.
6. Dixon, W. J.; Hayes, J. J.; Levin, J. R.; Weidner, M. F.; Dombroski, B. A.; Tullius, T. D. Methods Enzymol. 1991, 208, 380-413.
7. Balasubramanian, B.; Pogozelski, W. K.; Tullius, T. D. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 9738-9743.
8. Carey, J. Methods Enzymol. 1995, 259, 452-46.
9. Hayes, J. J.; Kam, L.; Tullius, T. D. Methods Enzymol. 1990, 186, 545-549.
10. Kolkhof, P.; Müller-Hill, B. J. Mol. Biol. 1994, 242, 23-36.
11. R1 operator sequence into a different expression vector for lambda repressor.
12. Beamer, L. J.; Pabo, C. O. J. Mol. Biol. 1992, 227, 177-196.
13. Ptashne, M. A Genetic Switch: Gene Control and Phage Lambda; Cell Press and Blackwell Scientific Publications: Cambridge, 1987.
14. Hayes, J. J.; Tullius, T. D. Biochemistry 1989, 28, 9521-9527.
15. (a) Sasse-Dwight, S.; Gralla, J. D. Methods Enzymol. 1991, 208, 146-168.
16. (b) Gralla, J. D. Proc. Natl. Acad. Sci U.S.A. 1985, 82, 3078-3081.
17. R polymerase (New England Biolabs).
18. 137Cs γ-ray source (Oncology Department, Johns Hopkins School of Medicine).The exposure rate was approximately 50 Gy/min. After irradiation cells were immediately lysed, and the DNA was isolated by standard methods.
19. Shadle, S. E.; Allen, D. F.; Guo, H.; Pogozelski, W. K.; Bashkin, J. S.; Tullius, T. D. Nucleic Acids Res. 1997, 25, 850-861.
20. (a) Tullius, T. D. Comprehensive Supramolecular Chemistry; Lehn, J.-M., Ed.; Pergamon: London, 1996; Vol. 5 (Bioinorganic Systems), pp 317-343.
21. (b) Draganescu, A.; Tullius, T. D. Metal Ions in Biological Systems; Sigel, H., Sigel, A., Eds.; Marcel Dekker: New York, 1996; Vol. 33, pp 453-484.
22. Hayes, J. J.; Tullius, T. D.; Wolffe, A. P. Proc. Natl. Acad. Sci. U.S.A. 1990, 87, 7405-7409.