Mechanisms for auto-inhibition and forced product release in glycine N-methyltransferase: Crystal structures of wild-type, mutant R175K and S-adenosylhomocysteine-bound R175K enzymes

Journal of Molecular Biology

Volumn 298, Issue 1, 2000, Pages 149-162

  Huang, Yafei ^a   Komoto, Junichi ^a   Konishi, Kiyoshi ^b   Takata, Yoshimi ^b   Ogawa, Hirofumi ^b   Gomi, Tomoharu ^b   Fujioka, Motoji ^b   Takusagawa, Fusao ^a  

^a UNIVERSITY OF KANSAS   (United States)

^b UNIVERSITY OF TOYAMA   (Japan)

Author keywords

Auto inhibition mechanism; Enzyme structure; Forced product release mechanism; Glycine N methyltransferase; X ray diffraction

Indexed keywords

GLYCINE; GLYCINE METHYLTRANSFERASE; METHYLTRANSFERASE; S ADENOSYLHOMOCYSTEINE; S ADENOSYLMETHIONINE; SARCOSINE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING SITE; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME ACTIVE SITE; ENZYME RELEASE; ENZYME STRUCTURE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN QUATERNARY STRUCTURE; SITE DIRECTED MUTAGENESIS;

EID: 0034696755     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3637     Document Type: Article

References (48)

1. Refined crystal structure of dogfish M4 apo-lactate dehydrogenase
2. Hepatic one-carbon metabolism in early folate deficiency in rats
3. Functional size of C-terminal protein carboxyl methyltransferase from kidney basolateral plasma membranes
4. Crystal structure of ErmC', an rRNA methyltransferase which mediates antibiotic resistance in bacteria
5. + ternary complex. Substrate binding and a model for the transition state
6. Crystal structure of the Hhal DNA methyltransferase complexed with S-adenosyl-L-methionine
7. Glycine N-methyltransferase is a folate binding protein of rat liver cytosol
8. Effect of dietary methyl group deficiency on one-carbon metabolism in rats
9. Crystal structure of the unactivated form of ribulose-1,5-bisphosphate carboxylase/oxygenase from tobacco refined at 2.0 Å resolution
10. Crystal structure of the chemotaxis receptor methyltransferase CheR suggests a conserved structural motif for binding S-adenosylmethionine
11. Analysis of hydride transfer and cofactor fluorescence decay in mutants of dihydrofolate reductase: Possible evidence for participation of enzyme molecular motions in catalysis
12. Three-dimensional structure of a pyridoxal-phosphate-dependent enzyme, mitochondrial aspartate amino-transferase
13. Crystal structure of glycine N-methyltransferase from rat liver
14. Structure of PvuII DNA-(cytosine N4) methyltransferase, an example of domain permutation and protein fold assignment
15. Purification and characterization of glycine N-methyltransferase
16. The 1.85 Å structure of vaccinia protein VP39: A bifunctional enzyme that participates in the modification of both mRNA ends
17. Three-dimensional structure of the tryptophan synthase alpha 2 beta 2 multienzyme complex from Salmonella typhimurium
18. Purification and properties of S-adenosyl-L-methionine: L-methionine S-methyltransferase from Wollastonia biflora leaves
19. Modulation of tRNA methyltransferase activity by competing enzyme systems
20. Chemical modification of a functional arginine residue of rat liver glycine methyltransferase
21. 2-terminal segment
22. Rapid and efficient site-specific mutagenesis with-out phenotypic selection
23. Three-dimensional structure of the adenine specific DNA methyltransferase M Taq I in complex with the cofactor S-adenosylmethionine
24. Molecular analysis of the dmpM gene encoding an O-demethyl puromycin O-methyltransferase from Streptomyces alboniger
25. PROCHECK: A program to check the stereochemical quality of protein structures
26. Crystallographic analysis of the complex between triosephosphate isomerase and 2-phosphoglycolate at 2.5 Å resolution: Implications for catalysis
27. Structures of the 'open' and 'closed' state of trypanosomal triosephosphate isomerase, as observed in a new crystal form: Implications for the reaction mechanism
28. Induction of rat liver glycine methyltransferase by high methionine diet
29. Purification and properties of glycine N-methyltransferase from rat liver
30. Mammalian glycine N-methyltransferases. Comparative kinetic and structural properties of the enzymes from human, rat, rabbit and pig livers
31. Recombinant expression of rat glycine N-methyltransferase and evidence for contribution of N-terminal acetylation to co-operative binding of S-adenosylmethionine
32. Processing of X-ray diffraction data collected in oscillation mode
33. Crystal structure of apo-glycine N-methyltransferase (GNMT)
34. Rat liver cytosolic 4 S polycyclic aromatic hydrocarbon binding protein is glycine N-methyltransferase
35. Glycine N-methyltransferase is a mediator of cytochrome P450 1A1 gene expression
36. The crystal structure of HaeIII methyltransferase covalently complexed to DNA. An extra-helical cytosine and rearranged base paring
37. Segmental movement: Definition of the structural requirements for loop closure in catalysis by triosephosphate isomerase
38. Universal catalytic domain structure of AdoMet-dependent methyltransferase
39. Rat guanidinoacetate methyltransferase. Effect of site-directed alteration of an aspartic acid residue that is conserved across most mammalian S-adenosylmethionine-dependent methyltransferases
40. A combined isotropic and multiple S-shell anisotropic scaling method for multiple data sets
41. Glycine N-methyltransferase, a terameric enzyme
42. An automatic diffraction data collection system with an imaging plate
43. Crystal structures and molecular conformations of L-methionine and L-norleusine
44. Crystal structure of the DpnM DNA adenine methyltransferase from the DpnII restriction system of Streptococcus pneumoniae bound to S-adenosylmethionine
45. Crystal structure of catechol O-methyltransferase
46. Inhibition of glycine N-methyltransferase activity by folate derivatives. Implication for regulation of methyl group metabolism
47. Solution structure of an rRNA methyltransferase (ErmAM) that confers macrolide-lincosamide-streptogramin antibiotic resistance