Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 in the biosynthesis of the cyanogenic glucoside dhurrin

Plant Molecular Biology

Volumn 36, Issue 3, 1998, Pages 393-405

  Bak, Søren ^a   Kahn, Rachel Alice ^a   Nielsen, Hanne Linde ^a   Møller, Birger Lindberg ^a   Halkier, Barbara Ann ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Cyanogenic glucosides; Cytochrome P450; E. coli expression; Oxime reconstitution; PCR

Indexed keywords

CYTOCHROME P 450 CYP71E1 (SORGHUM); CYTOCHROME P 450 CYP98A1 (SORGHUM BICOLOR); CYTOCHROME P 450 CYP99A1(SORGHUM BICOLOR); CYTOCHROME P-450 CYP71E1 (SORGHUM); CYTOCHROME P-450 CYP98A1 (SORGHUM BICOLOR); CYTOCHROME P-450 CYP99A1(SORGHUM BICOLOR); CYTOCHROME P450; DHURRIN; NITRILE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CEREAL; CHEMISTRY; CONSENSUS SEQUENCE; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; CEREALS; CLONING, MOLECULAR; CONSENSUS SEQUENCE; CYTOCHROME P-450 ENZYME SYSTEM; KINETICS; MOLECULAR SEQUENCE DATA; NITRILES; PHYLOGENY; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID;

EMBRYOPHYTA; ESCHERICHIA COLI; SORGHUM BICOLOR;

EID: 0032005917     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005915507497     Document Type: Article

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