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Volumn 483, Issue 2-3, 2000, Pages 139-142
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Identification of the histidine and aspartic acid residues essential for enzymatic activity of a family I.3 lipase by site-directed mutagenesis
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Author keywords
Active site; Catalytic triad; Lipase; Pseudomonas; Site directed mutagenesis
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Indexed keywords
ALANINE;
AMINO ACID;
ASPARTIC ACID;
HISTIDINE;
SERINE;
TRIACYLGLYCEROL LIPASE;
AMINO ACID SEQUENCE;
ARTICLE;
CATALYSIS;
CIRCULAR DICHROISM;
ENZYME ACTIVE SITE;
NONHUMAN;
PRIORITY JOURNAL;
PROTEIN TERTIARY STRUCTURE;
PSEUDOMONAS;
SEQUENCE ANALYSIS;
SITE DIRECTED MUTAGENESIS;
AMINO ACID SUBSTITUTION;
ASPARTIC ACID;
BINDING SITES;
CATALYTIC DOMAIN;
CIRCULAR DICHROISM;
ESTERASES;
HISTIDINE;
LIPASE;
MUTAGENESIS, SITE-DIRECTED;
MUTATION;
PSEUDOMONAS;
SUPPORT, NON-U.S. GOV'T;
PSEUDOMONAS;
PSEUDOMONAS SP.;
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EID: 0034693168
PISSN: 00145793
EISSN: None
Source Type: Journal
DOI: 10.1016/S0014-5793(00)02103-7 Document Type: Article |
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Times cited : (26)
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References (25)
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