Comparison of methods for thermolysin-catalyzed peptide synthesis including a novel more active catalyst

Biotechnology and Bioengineering

Volumn 69, Issue 6, 2000, Pages 633-638

  Ulijn, Rein V ^a   Erbeldinger, Markus ^a   Halling, Peter J ^a  

^a UNIVERSITY OF STRATHCLYDE   (United Kingdom)

Author keywords

Low water media; Organic media; Peptide synthesis; Solid to solid; Thermolysin

Indexed keywords

ACETONITRILE; ALCOHOLS; BIOSYNTHESIS; CATALYSIS; CATALYSTS; COMPOSITION EFFECTS; CONFORMATIONS; ENZYME IMMOBILIZATION; POLYPROPYLENES; PRECIPITATION (CHEMICAL); SILICA; WATER;

PEPTIDES; PROPANOL; THERMOLYSIN;

AMINO ACIDS;

ACETONITRILE; INFUSORIAL EARTH; PEPTIDE; POLACRILIN; POLYPROPYLENE; PROPANOL; SILICON DIOXIDE; THERMOLYSIN;

AQUEOUS SOLUTION; ARTICLE; BIOTECHNOLOGY; CATALYST; CHEMICAL REACTION KINETICS; CONFORMATIONAL TRANSITION; ENZYME ANALYSIS; ENZYME IMMOBILIZATION; HYDROLYSIS; INTERMETHOD COMPARISON; PEPTIDE SYNTHESIS; SUSPENSION;

EID: 0034692620     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0290(20000920)69:6<633::AID-BIT7>3.0.CO;2-F     Document Type: Article

References (14)

1. Effects of acetonitrile-water mixtures on α-chymotrypsin catalysed dipeptide synthesis
2. Effect of thermodynamic water activity on thermolysin-catalysed peptide synthesis in organic two-phase systems
3. Dramatic enhancement of enzymatic activity in organic solvents by lyoprotectants
4. Effect of water and enzyme concentration on thermolysin catalysed solid-to-solid peptide synthesis
5. Biologically active peptide and enzymatic approaches to their production
6. Nonconventional protease catalysis in frozen aqueous solutions
7. Non-conventional enzyme catalysis: Application of proteases and zymogens in biotransformations
8. On enzymatic activity in organic solvents as a function of enzyme history
9. Enzymatic reactions in aqueous-organic media. VI. Peptide synthesis by α-chymotrypsin in hydrophilic organic solvents
10. Why are enzymes in organic solvents less active than in water?
11. A practical route to high activity enzyme preparations for synthesis in organic media
12. Optimization of Pseudomonas cepacia lipase preparations for catalysis in organic solvents
13. Enzyme-catalyzed peptide synthesis in ice
14. The effect of water-content and nature of organic solvent on enzyme activity in low water media - A quantitative description