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Volumn 288, Issue 5469, 2000, Pages 1257-1259

Impaired prion replication in spleens of mice lacking functional follicular dendritic cells

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; B LYMPHOCYTE; CONTROLLED STUDY; DENDRITIC CELL; MOUSE; NONHUMAN; PRION; PRIORITY JOURNAL; SCRAPIE; SPLEEN; STROMA; T LYMPHOCYTE; VIRUS INFECTION; VIRUS REPLICATION;

EID: 0034686002     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.288.5469.1257     Document Type: Article
Times cited : (326)

References (46)
  • 5
    • 0031444295 scopus 로고    scopus 로고
    • M. A. Klein et al., Nature 390, 687 (1997).
    • (1997) Nature , vol.390 , pp. 687
    • Klein, M.A.1
  • 6
    • 0033577717 scopus 로고    scopus 로고
    • A. J. Raeber et al., EMBO J. 18, 2702 (1999).
    • (1999) EMBO J. , vol.18 , pp. 2702
    • Raeber, A.J.1
  • 16
    • 0030917003 scopus 로고    scopus 로고
    • P. A. Koni et al., Immunity 6, 491 (1997).
    • (1997) Immunity , vol.6 , pp. 491
    • Koni, P.A.1
  • 19
    • 0027415109 scopus 로고
    • J. L. Browning et al., Cell 72, 847 (1993).
    • (1993) Cell , vol.72 , pp. 847
    • Browning, J.L.1
  • 24
    • 0032842326 scopus 로고    scopus 로고
    • Q. Wu et al., J. Exp. Med. 190, 629 (1999).
    • (1999) J. Exp. Med. , vol.190 , pp. 629
    • Wu, Q.1
  • 25
    • 0342335729 scopus 로고    scopus 로고
    • note
    • 50 units of Rocky Mountain Laboratory (RML) scrapie prions. Mice were monitored every second day, and scrapie was diagnosed according to standard clinical criteria (6).
  • 26
    • 0343640929 scopus 로고    scopus 로고
    • note
    • 50 units of mouse-adapted scrapie prions (RML 4.1). Depletion was maintained by weekly administration of 100 μg of the fusion protein for 8 weeks. A second set of mice were inoculated with prions as above; 300 μg of LTβR-lg were injected 1 week later and 100 μg LTβR-lg were injected once a week for a further 6 weeks. Three mice from each group were culled 1, 3, and 8 weeks after inoculation, respectively. As control, C57BL/6 mice were subjected to the same regime with a human IgG polyclonal preparation (hulgG, Sandoglobulin) instead of LTβR-lg.
  • 27
    • 0343205314 scopus 로고    scopus 로고
    • data not shown
    • F. Montrasio et al., data not shown.
    • Montrasio, F.1
  • 28
    • 0343205312 scopus 로고    scopus 로고
    • personal communication
    • M. Kosco-Vilbois, personal communication.
    • Kosco-Vilbois, M.1
  • 34
    • 0342770669 scopus 로고    scopus 로고
    • note
    • Spleen homogenates (10% w/v) from frozen tissues were prepared in 2% sarkosyl-phosphate-buffered saline (PBS). Homogenates were adjusted to 8 mg/ml protein. Total protein (200 μg) of each spleen was treated with proteinase K (20 μg/ml, 30 min, 37°C). Samples were subjected to electrophoresis through an SDS-polyacrylamide gel (12.5%), transferred to nitrocellulose membranes, and probed with polyclonal antiserum 1B3 to mouse PrP (44), and bound antibody was visualized by enhanced chemiluminescence (SuperSignal West Dura Extended Duration Substrate, Pierce).
  • 36
    • 0029863648 scopus 로고    scopus 로고
    • M. Fischer et al., EMBO J. 15, 1255 (1996).
    • (1996) EMBO J. , vol.15 , pp. 1255
    • Fischer, M.1
  • 37
    • 0030054010 scopus 로고    scopus 로고
    • S. Brandner et al., Nature 379, 339 (1996).
    • (1996) Nature , vol.379 , pp. 339
    • Brandner, S.1
  • 41
    • 0024519176 scopus 로고
    • ............, Virus Res. 12, 201 (1989).
    • (1989) Virus Res. , vol.12 , pp. 201
  • 46
    • 0343205304 scopus 로고    scopus 로고
    • note
    • We thank M. Kosco for the gift of FDC M1 antibody and C. Musahl for the rabbit anti-PrP antiserum XN. Supported by the Kanton of Zürich and by Schweizerischer Nationalfonds and European Union grants to A.A. and C.W.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.