Inhibiting proteasomes in human HepG2 and LNCaP cells increases endogenous androgen receptor levels

Biochemical and Biophysical Research Communications

Volumn 276, Issue 1, 2000, Pages 144-150

  Sheflin, Lowell ^a   Keegan, Brendan ^a   Zhang, Wan ^a   Spaulding, Stephen W ^a  

^a UNIVERSITY AT BUFFALO   (United States)

Author keywords

Androgen receptor; GSK3 ; HepG2; LNCaP; MG132; PEST; Proteasome; Ubiquitination; Catenin

Indexed keywords

ANDROGEN RECEPTOR; LACTACYSTIN; PROTEASOME; PROTEIN;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; HUMAN; HUMAN CELL; IMMUNOREACTIVITY; MOLECULAR WEIGHT; PRIORITY JOURNAL; PROTEIN CONTENT; RECEPTOR DENSITY; REGULATORY MECHANISM;

MAMMALIA;

EID: 0034675275     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2000.3424     Document Type: Article

References (39)

1. Specificity of ligand-dependent androgen receptor stabilization: Receptor domain interactions influence ligand dissociation and receptor stability
2. Identification of three proline-directed phosphorylation sites in the human androgen receptor
3. Androgen receptor phosphorylation, turnover, nuclear transport, and transcriptional activation. Specificity for steroids and antihormones
4. Differential posttranscriptional regulation of androgen receptor gene expression by androgen in prostate and breast cancer cells
5. Transcriptional and posttranscriptional regulation of human androgen receptor by androgen
6. Steroid receptor interactions with heat shock protein and immunophilin chaperones
7. Hsp90 regulates androgen receptor hormone binding affinity in vivo
8. Hormone-induced dissociation of the androgen receptor-heat-shock protein complex: Use of a new monoclonal antibody to distinguish transformed from nontransformed receptors
9. Mechanisms of androgen receptor activation and function
10. A and B forms of the androgen receptor are expressed in a variety of human tissues
11. Phosphorylation/dephosphorylation of androgen receptor as a determinant of androgen agonistic or antagonistic activity
12. Expression of androgen receptor and its implication in hepatoma cells
13. Regulation of androgen receptor protein and mRNA concentrations by androgens in rat ventral prostate and seminal vesicles and in human hepatoma cells
14. 3 receptors by blocking ubiquitin/proteasome-mediated degradation in human skin
15. Evidence for enhanced ubiquitin-mediated proteolysis of the chicken progesterone receptor by progesterone
16. Proteasome-dependent degradation of the human estrogen receptor
17. Ubiquitination of the rat uterine estrogen receptor: Dependence on estradiol
18. Proteasome-mediated proteolysis of estrogen receptor: A novel component in autologous down-regulation
19. PEST sequences and regulation by proteolysis
20. Childhood hepatoblastomas frequently carry a mutated degradation targeting box of the β-catenin gene
21. Serine phosphorylation regulated ubiquitination and degradation of β-catenin
22. β-Catenin is a target for the ubiquitin-proteasome pathway
23. Glycogen synthase kinase-3: Functions in oncogenesis and development
24. Domain requirements of DnaJ-like (Hsp40) molecular chaperones in the activation of a steroid hormone receptor
25. Polyglutamine-expanded androgen receptors form aggregates that sequester heat shock proteins, proteasome components and SRC-1, and are suppressed by the HDJ-2 chaperone
26. The CAG repeat within the androgen receptor gene and its relationship to prostate cancer
27. Sequential phosphorylation by mitogen-activated protein kinase and glycogen synthase kinase 3 represses transcriptional activation by heat shock factor-1
28. Mapping of the inducible IkB phosphorylation sites that signal its ubiquitination and degradation
29. Androgen receptor phosphorylation
30. Androgen receptor: An overview
31. Forskolin-induced dephosphorylation of the androgen receptor impairs ligand binding
32. Role of phosphorylation in determining the backbone dynamics of the serine/threonine-proline motif and Pin1 substrate recognition
33. Substrates containing phosphorylated residues adjacent to proline decrease the cleavage by proline-specific peptidases
34. Identification of a novel RING finger protein as a coregulator in steroid receptor-mediated gene transcription
35. The Angelman syndrome-associated protein, E6-AP, is a coactivator for the nuclear hormone receptor superfamily
36. Ubc9 interacts with the androgen receptor and activates receptor-dependent transcription
37. RING fingers mediate ubiquitin-conjugating enzyme (E2)-dependent ubiquitination
38. Characterization of the mUBC9-binding sites required for E2A protein degradation
39. SUMO-1 modification of the acute promyelocytic leukaemia protein PML: Implications for nuclear localisation