An immuno-electron microscopical analysis of transcribing multinucleosomal templates: What happens to the histones?

Journal of Molecular Biology

Volumn 299, Issue 4, 2000, Pages 853-858

  Ten Heggeler Bordier, Béatrice ^a   Muller, Sylviane ^b   Monestier, Marc ^c   Wahli, Walter ^a  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

^b CNRS   (France)

^c TEMPLE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Antihistone monoclonal autoantibodies; Chromatin; Colloidal gold; Immuno electron microscopy; Transcription

Indexed keywords

AUTOANTIBODY; COLLOIDAL GOLD; DNA; EPITOPE; HISTONE; MONOCLONAL ANTIBODY; RNA 5S; RNA POLYMERASE;

ANTIGEN ANTIBODY REACTION; ARTICLE; BACTERIOPHAGE T7; CHROMATIN; CONTROLLED STUDY; DNA HISTONE INTERACTION; DNA TEMPLATE; IMMUNOELECTRON MICROSCOPY; IN VITRO STUDY; NONHUMAN; NUCLEOSOME; PRIORITY JOURNAL; RNA TRANSCRIPTION; X RAY DIFFRACTION;

EID: 0034674153     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3790     Document Type: Article

References (23)

1. The organization of histones and DNA in chromatin: Evidence for an arginine-rich histone kernel
2. Reversible and irreversible changes in nucleosome structure along the c-fos and c-myc oncogenes following inhibition of transcription
3. A nucleosome core is transferred out of the path of a transcribing polymerase
4. Role of chromatin structure in the regulation of transcription by RNA polymerase II
5. A new preparation method for dark-field electron microscopy of bio-macromolecules
6. The interaction of transcription factors with nucleosomal DNA
7. In vitro transcription trough nucleosomes by T7 RNA polymerase
8. A bacteriophage RNA polymerase transcribes in vitro through a nucleosome core without displacing it
9. Crystal structure of the nucleosome core particle at 2.8 Angstrom resolution
10. Structure and binding properties of monoclonal antibodies to core histones from autoimmune mice
11. Nucleosome arrays inhibit both initiation and elongation of transcripts by bacteriophage T7 RNA polymerase
12. Measurement of the frequency of histone displacement during in vitro transcription of nucleosomes: RNA is a competitor for these histones
13. In vitro evidence that transcription-induced stress causes nucleosome dissolution and regeneration
14. An ELISA for detecting aptoptosis
15. A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite
16. Chromatin reconstituted from tandemly repeated cloned DNA fragments and the core histones: A model system for study of higher order structure
17. Mapping of linear histone regions exposed at the surface of the nucleosome in solution
18. Overcoming a nucleosomal barrier to transcription
19. Visualization of RNA polymerase II ternary transcription complexes formed in vitro on a Xenopus laevis vitellogenin gene
20. Immuno-electron microscopic identification of human estrogen receptor-DNA complexes at the estrogen-responsive element and in the first intron of a Xenopus vitellogenin gene
21. Fate of linear and supercoiled multinucleosomic templates during transcription
22. What happens to nucleosomes during transcription
23. Chromatin structure of a hyperactive secretory protein (in Balbiani ring 2) of Chironomus