Retrograde and anterograde tracing combined with transmitter identification and electron microscopy

Journal of Neuroscience Methods

Volumn 103, Issue 1, 2000, Pages 117-126

  Deller, Thomas ^a   Naumann, Thomas ^a   Frotscher, Michael ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Fiber tract; Fluoro Gold; Hippocampus; Phaseolus vulgaris leucoagglutinin; Postembedding immunocytochemistry

Indexed keywords

NEUROTRANSMITTER;

ANALYTIC METHOD; ANIMAL TISSUE; ARTICLE; ELECTRON MICROSCOPY; IMMUNOCYTOCHEMISTRY; NERVE FIBER; NEUROANATOMY; NEUROCHEMISTRY; NONHUMAN; PRIORITY JOURNAL; ULTRASTRUCTURE;

ANIMALS; AXONAL TRANSPORT; AXONS; BRAIN; FLUORESCENT DYES; IMMUNOHISTOCHEMISTRY; MICROSCOPY, ELECTRON; NEURAL PATHWAYS; NEURONS; NEUROTRANSMITTER AGENTS; PHYTOHEMAGGLUTININS; STILBAMIDINES; SYNAPSES;

EID: 0034669864     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(00)00301-0     Document Type: Article

References (48)

1. Preparation of central nervous system tissue for light and electron microscopy
2. The rostral dorsal cap and ventrolateral outgrowth of the rabbit inferior olive receive a GABAergic input from dorsal group Y and the ventral dentate nucleus
3. Ultrastructural study of the GABAergic, cerebellar, and mesodiencephalic innervation of the cat medial accessory olive: Anterograde tracing combined with immunocytochemistry
4. Reciprocal connections of lateral septal neurons and neurons in the lateral hypothalamus in the rat: A combined Phaseolus vulgaris-leucoagglutinin and Fluoro-Gold immunocytochemical study
5. Morphological evidence for the sprouting of inhibitory commissural fibers in response to the lesion of the excitatory entorhinal input to the rat dentate gyrus
6. Phaseolus vulgaris leucoagglutinin (PHAL) tracing of commissural fibers to the rat dentate gyrus: Evidence for a previously unknown commissural projection to the outer molecular layer
7. A novel entorhinal projection to the rat dentate gyrus: Direct innervation of proximal dendrites and cell bodies of granule cells and GABAergic neurons
8. Heterogeneity of the commissural projection to the rat dentate gyrus: A Phaseolus vulgaris leucoagglutinin tracing study
9. Different primary target cells are important for fiber lamination in the fascia dentata: A lesson from reeler mutant mice
10. GABAergic septohippocampal neurons contain parvalbumin
11. GABAergic septal and serotonergic median raphe afferents preferentially innervate inhibitory interneurons in the hippocampus and dentate gyrus
12. Anterograde PHA-L tracing combined with pre- and postembedding immunocytochemistry
13. GABA-containing neurons in the septum control inhibitory interneurons in the hippocampus
14. Morphological characteristics of glutamatergic synapses in the hippocampus
15. An anterograde neuroanatomical tracing method that shows the detailed morphology of neurons, their axons and terminals: Immunohistochemical localization of an axonally transported plant lectin, Phaseolus vulgaris leucoagglutinin (PHAL)
16. GABAergic neurons in the entorhinal cortex project to the hippocampus
17. Light and electron microscopic tracing of neuronal connections with Phaseolus vulgaris leucoagglutinin (PHA-L), and combinations with other neuroanatomical techniques
18. Calretinin is present in non-pyramidal cells of the rat hippocampus I. A new type of neuron specifically associated with the mossy fibre system
19. Antisera to gamma-aminobutyric acid. I. Production and characterization using a new model system
20. Immunocytochemistry II. Post-embedding staining
21. Fate of GABAergic septohippocampal neurons after fimbria-fornix transection as revealed by in situ hybridization for glutamate decarboxylase mRNA and parvalbumin immunocytochemistry
22. High-pressure freezing and freeze-substitution of native rat brain: Suitability for preservation and immunoelectron microscopic localization of myelin glycolipids
23. Septal neurons containing glutamatic acid decarboxylase immunoreactivity project to the hippocampal region in the rat brain
24. Multiple neuroanatomical tracing in primates
25. A review of rapid microwave fixation technology: Its expanding niche in morphologic studies
26. Principal cells are the postsynaptic targets of supramammillary afferents in the hippocampus of the rat
27. Organization of AMPA receptor subunits at a glutamate synapse: A quantitative immunogold analysis of hair cell synapses in the rat organ of Corti
28. Improved preservation of ultrastructure in difficult-to-fix organisms by high pressure freezing and freeze substitution: I. Drosophila melanogaster and Strongylocentrotus purpuratus embryos
29. Immunoelectronmicroscopy of soluble and membrane proteins with a sensitive postembedding method
30. Fine structure of rat septohippocampal neurons: II. A time course analysis following axotomy
31. Fine structure of rat septohippocampal neurons: I. Identification of septohippocampal projection neurons by retrograde tracing combined with electron microscopic immunocytochemistry and intracellular staining
32. Fine structure of rat septohippocampal neurons: III. Recovery of choline acetyltransferase immunoreactivity after fimbria-fornix transection
33. Multiple projections are unlikely to account for the survival of rat medial septal neurons after axotomy
34. Retrograde tracing with Fluoro-Gold: Different methods of tracer detection at the ultrastructural level and neurodegenerative changes of back-filled neurons in long-term studies
35. Quantitative electron microscopic immunocytochemistry of neuroactive amino acids
36. An osmium-free method of epon embedment that preserves both ultrastructure and antigenicity for post-embedding immunocytochemistry
37. A GABAergic inhibitory component within the hippocampal commissural pathway
38. Fluoro-Gold: A new fluorescent retrograde axonal tracer with numerous unique properties
39. Neurons containing retrogradely transported Fluoro-Gold exhibit a variety of lysosomal profiles: A combined brightfield, fluorescence, and electron microscopic study
40. Projection from the cerebellar lateral nucleus to precerebellar nuclei in the mossy fiber pathway is glutamatergic: A study combining anterograde tracing with immunogold labeling in the rat
41. Anterograde tracing with PHA-L and biocytin at the electron microscopic level
42. Intra-amygdaloid projections of the lateral nucleus in the cat: PHA-L anterograde labeling combined with postembedding GABA and glutamate immunocytochemistry
43. Antisera to gamma-aminobutyric acid. III. Demonstration of GABA in golgi-impregnated neurons and in conventional electron microscopic sections of cat striate cortex
44. Immunocytochemistry I: Pre-embedding staining
45. Inhibitory synaptic inputs to the oculomotor nucleus from vestibulo-ocular-reflex-related nuclei in the rabbit