Measuring the quantity and activity of mitochondrial electron transport chain complexes in tissues of central nervous system using blue native polyacrylamide gel electrophoresis

Analytical Biochemistry

Volumn 286, Issue 2, 2000, Pages 214-223

  Jung, Cheolwha ^a   Higgins, Cynthia M J ^a   Xu, Zuoshang ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

ALS; ATP; Energy; Neurodegenerative disease; Neuronal degeneration; Respiratory enzyme

Indexed keywords

ADENOSINETRIPHOSPHATE; ALUMINUM; ELECTRON TRANSPORT PROPERTIES; ELECTROPHORESIS; ENZYMES; FREE RADICALS; HISTOLOGY; MITOCHONDRIA; NEUROPHYSIOLOGY; TISSUE;

ENERGY; MITOCHONDRIAL DYSFUNCTION; MITOCHONDRIAL ELECTRON TRANSPORT CHAIN; NEURODEGENERATIVE DISORDERS; NEURONAL DEGENERATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; RESPIRATORY ENZYMES; SPECTROPHOTOMETRIC ASSAYS;

NEURODEGENERATIVE DISEASES;

CYTOCHROME C OXIDASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE (UBIQUINONE); SUCCINATE DEHYDROGENASE (UBIQUINONE); UBIQUINOL CYTOCHROME C REDUCTASE;

ANIMAL TISSUE; ARTICLE; CENTRAL NERVOUS SYSTEM; CEREBELLUM; FOREBRAIN; HISTOCHEMISTRY; MITOCHONDRIAL ENERGY TRANSFER; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SPINAL CORD;

ANIMALIA;

EID: 0034669552     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4813     Document Type: Article

References (24)

1. Mitochondria, free radicals, and neurodegeneration
2. An evaluation of the role of mitochondria in neurodegenerative diseases: Mitochondrial mutations and oxidative pathology, protective nuclear responses, and cell death in neurodegeneration
3. Massive mitochondrial degeneration in motor neurons triggers the onset of amyotrophic lateral sclerosis in mice expressing a mutant SOD1
4. Electron transport chain defects in Alzheimer's disease brain
5. Mitochondrial oxidative phosphorylation defects in Parkinson's disease
6. Evidence in a lethal infantile mitochondrial disease for a nuclear mutation affecting respiratory complexes I and IV
7. Metabolic dysfunction in familial, but not sporadic, amyotrophic lateral sclerosis
8. Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form
9. Electrophoretic techniques for isolation and quantification of oxidative phosphorylation complexes from human tissues
10. Separation by blue native and colorless native polyacrylamide gel electrophoresis of the oxidative phosphorylation complexes of yeast mitochondria solubilized by different detergents: Specific staining of the different complexes
11. Analysis of oxidative phosphorylation complexes in cultured human fibroblasts and aminocytes by blue-native-electrophoresis using mitoplasts isolated with the help of digitonin
12. Flavinylation of succinate: Ubiquinone oxidoreductase from Saccharomyces cerevisiae
13. Quantification of muscle mitochondrial oxidative phosphorylation enzymes via histochemical staining of blue native polyacrylamide gels
14. Nondroplet ultrastructural demonstration of cytochrome oxidase activity with a polymerizing osmiophilic reagent, diaminobenzidine (DAB)
15. Mitochondrial electron-transport inhibitors
16. Proteins, polypeptides, prosthetic groups, and enzymic properties of complexes I, II, III, IV, and V of the mitochondrial oxidative phosphorylation system
17. Assessment of mitochondrial oxidative phosphorylation in patient muscle biopsies, lymphoblasts, and transmitochondrial cell lines
18. Assay conditions for the mitochondrial NADH: Coenzyme Q oxidoreductase
19. Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis